The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 2, 2025

Background: Abnormal expression of Zinc finger (ZNF) genes is commonly observed in osteosarcoma (OS), the most prevalent malignant bone tumor children and teenagers. This project focused on role ZNF560 progress OS. Methods: The published datasets including TCGA-SARC GSE99671 was utilized to screen out abnormal associated gene patterns sarcoma OS tissues. Prognosis value identified cohorts. In order manipulate HOS MG63 (OS) cells, genetic strategies such as shRNA constructs were utilized. analyzed through techniques immunohistochemistry, Western blotting, qRT-PCR. Results: By analyzing data from both GEO Cancer Genome Atlas (TCGA) databases, increased tissues verified, which significantly with poorer outcomes patients our own Additionally, downregulation resulted decreased cell viability, fewer colonies, induced apoptosis cells. Moreover, found be essential for migration human Conclusion: Collectively, these findings suggest that has potential serve a predictive biomarker osteosarcoma.

Язык: Английский

---

Zinc Finger Proteins in Colorectal Cancer: Insights into Molecular Mechanisms and Therapeutic Implications

Technology in Cancer Research & Treatment, Год журнала: 2025, Номер 24

Опубликована: Апрель 1, 2025

Colorectal cancer ranks as one of the most common malignancies globally, with mortality rates second only to lung cancer. Despite improvements in diagnostic and therapeutic approaches, long-term survival for colorectal patients remain unsatisfactory. The onset progression this disease involve intricate molecular mechanisms, influenced by a range biological factors. Zinc finger proteins play critical role these processes, impacting tumor development patient prognosis. This review summarizes current research on zinc cancer, highlighting key findings advancements understanding their biology.

Язык: Английский

---

Selective targeting of genes regulated by zinc finger proteins in endometriosis and endometrioid adenocarcinoma by zinc niflumato complex with neocuproine

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 24, 2025

Inadequate angiogenesis of endometriotic implants stimulated by the inflammatory microenvironment in uterine region leads to development gynecological diseases, which significantly reduce fertility and vitality young women. Angiogenic processes are controlled factors whose activities regulated at gene level reactive oxygen species (ROS), hypoxia-induced (HIFs), zinc-finger proteins (ZnFs) or posttranscriptionally via non-coding RNAs. The cooperation these is responsible for manifestation pathological stimuli form endometriosis body uterus, ovaries, peritoneum, from endometrioid carcinoma can develop. Molecules that control expression their intercalation target DNA sequence, such as [Zn(neo)(nif)2], could prevent hyperactivation pro-angiogenic pathways (decrease HIF-1α, VEGF-A, TGF-β1, COX2, ANG2/ANG1), formation ROS, risk neoplasticity. NSAID-metal complex [Zn(neo)(nif)2] shows an ability intercalate into ZNF3-7 sequence a higher rate, explain its effect on genes this transcription factor. In addition, affects ROS production Ca2+ level, possibly pointing mitochondrial dysfunction potential cause described apoptosis.

Язык: Английский

---

Zinc Biofortification in Cereal Crops: Overview and Prospects

Journal of soil science and plant nutrition, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Язык: Английский

---

Molecular Functions and Potential Utilization of Zinc Finger Proteins in Protozoan Parasites

Опубликована: Апрель 10, 2025

Zinc finger proteins (ZFP) are metalloproteins whose zinc atom interacts with side chains of specific histidines and cysteines the motif, generating functional three dimensional structures. A good number these from different species similar while others divergent in structure function. Existing literature on structural features, functions their potential to be targeted for drug development or used as molecular tools parasitic protozoa was explored. Evidence reviewed articles revealed that, just like other eukaryotes, function various parasite cellular processes such as: transcription, RNA editing, mRNA processing & export and, turn-over among others. Some them had same features homologues eukaryotes including hosts. However, there were which studies aiming at replacing reported positive results some parasites Giardia lamblia, Trichomonas vaginalis Leishmania donovani though this is promising, off- target challenge that can cause effects, must solved before wide application achieved. Successful engineering use genome editing also Plasmodium falciparum. Since nucleases a powerful research medical tool, repurposing ZFPs useful therapy discovery. For succeed, more knowledge how regulated needed.

Язык: Английский

---

Transmembrane protein TMEM230, regulator of metalloproteins and motor proteins in gliomas and gliosis

Advances in protein chemistry and structural biology, Год журнала: 2024, Номер unknown, С. 255 - 297

Опубликована: Янв. 1, 2024

Язык: Английский

---

ZFP1 is a biomarker related to poor prognosis and immunity in gastric cancer

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Сен. 11, 2024

We aimed to determine the prognostic significance of ZFP1 in gastric cancer (GC), its role immune microenvironment, and potential as a therapeutic target using data from The Cancer Genome Atlas (TCGA) database. overexpression was closely associated with tumour T stage histological grade. Patients GC high expression had poor outcomes. Lower longer symptom-free intervals disease-specific survival. Subgroup analyses T3 T4, N0, N1, M0 patients showed that overall survival (OS), survival, progression-free interval (PFI) were worse those expression. moderately strongly positively correlated infiltration levels effector central memory cells helper negatively Th17 NK CD56bright cells. lncRNA-miRNA-ZFP1 axis predicted public CCK8, colony formation, wound healing assays conducted investigate whether promoted proliferation migration Our study suggests plays key prognosis, response, progression is significant factor diagnosis treatment this disease.

Язык: Английский

---

Investigating the Molecular Mechanisms Underlying Early Response to Inflammation and Helicobacter pylori Infection in Human Gastric Epithelial Cells

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15147 - 15147

Опубликована: Окт. 13, 2023

Helicobacter pylori is a leading cause of chronic gastric inflammation, generally associated with gastritis and adenocarcinoma. Activation the NF-κB pathway mainly contributes to inflammatory phenotype observed in H. infection humans experimental models. Since epithelium undergoes rapid turnover, inflammation pathogenicity result from early phase chronically activated pathways. In present study we investigated host response non-tumoral human epithelial cells (GES-1). To dissect pathogen-specific mechanisms also examined tumor necrosis factor (TNF), prototypical cytokine. By analyzing activation state signaling, cytokine expression secretion, transcriptome, found that GES-1 TNF results multiple pathways transcription factors, e.g., CCAAT/enhancer-binding proteins (CEBPs). comparing transcriptomic profiles, induces less potent than but affects gene greater extent by specifically inducing factors such as CEBPβ numerous zinc finger proteins. Our provides insights on cellular modulated unveiling new potential targets.

Язык: Английский

---

Biomarkers of Tumor Metastasis and Invasiveness

Cancers, Год журнала: 2023, Номер 15(20), С. 5000 - 5000

Опубликована: Окт. 16, 2023

The identification of proteins as new cancer diagnostic and prognostic biomarkers continues to attract considerable attention in the oncology literature, especially context invasion metastasis activation process [...].

Язык: Английский

---

Acetylcytidine modification of DDX41 and ZNF746 by N-acetyltransferase 10 contributes to chemoresistance of melanoma

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Авг. 23, 2024

Background Rapidly developed chemoresistance to dacarbazine (DTIC) is a major obstacle in the clinical management of melanoma; however, roles and mechanisms epi-transcriptomic RNA modification this process have not been investigated. Method DTIC-resistant (DR) melanoma cells were established for bulk sequencing. The expressions mRNAs detected using qRT-PCR, protein levels determined Western blotting immunohistochemistry. Acetylated RNAs by dot immunoprecipitation sequencing (acRIP-seq). A lung metastasis mouse model was evaluate anti-melanoma effects vivo . Results We identified that expression N -acetyltransferase 10 (NAT10), catalytic enzyme 4 -acetylcytidine (ac4C) RNA, significantly upregulated DR cells. Clinically, NAT10 elevated disease progression samples predicted poor outcome. Using ac4C (ac4C-RIP), we found two C2H2 zinc finger transcriptional factors, DDX41 ZNF746 , targets NAT10-mediated modification. Gain- loss-of-function experiments NAT10, or ZNF746, altered chemosensitivity accordingly, target genes also negatively correlated with outcomes. Finally, pharmacological inhibition Remodelin sensitized DTIC treatment vitro xenograft model. Conclusion Our study elucidates previously unrecognized role provides rationale developing new strategies overcome patients.

Язык: Английский

---