Effect of Chemical Injury on Autophagy in Canine Corneal Stromal Fibroblasts

Journal of Ocular Pharmacology and Therapeutics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Purpose: Ocular trauma leads to loss of corneal clarity resulting in vision deficits. Autophagy plays a critical role the extracellular matrix, tissue repair, and homeostasis but its precise mechanistic regulating function remains unknown. The present study investigated modulation autophagy-related genes (LC3, Beclin1, Sqstm1/p62, Lamp1) healthy injured canine stromal fibroblasts (CSFs).

Язык: Английский

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Differential gene expression and protein-protein interaction network profiling of sulfur mustard-exposed rabbit corneas employing RNA-seq data and bioinformatics tools

Experimental Eye Research, Год журнала: 2023, Номер 235, С. 109644 - 109644

Опубликована: Сен. 7, 2023

Язык: Английский

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Transcriptomic Landscape of Quiescent and Proliferating Human Corneal Stromal Fibroblasts

Experimental Eye Research, Год журнала: 2024, Номер 248, С. 110073 - 110073

Опубликована: Сен. 6, 2024

Язык: Английский

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Senescence and Stress Signaling Pathways in Corneal Cells After Nitrogen Mustard Injury

Cells, Год журнала: 2024, Номер 13(23), С. 2021 - 2021

Опубликована: Дек. 6, 2024

Mustard gas keratopathy (MGK), a complication of exposure to sulfur mustard, is blinding ocular surface disease involving key cellular pathways, including apoptosis, oxidative stress, and inflammation. Recent studies indicate that senescence contributes the pathophysiology mustard toxicity. This study aimed assess stress-related pathways-particularly mitogen-activated protein kinase (MAPK) signaling-in nitrogen (NM)-induced corneal injury. In vitro, primary human epithelial (P-HCECs), mesenchymal stromal cells (hcMSCs), corneal-limbal cell (HCLE) lines were exposed varying concentrations NM. The results demonstrated dose-dependent increase in senescence, characterized by reduced Ki67 expression, elevated p16, p21 mRNA levels, as well activation MAPK pathway activation. Treatment with selective p38-MAPK inhibitor significantly markers improved proliferation following Overall, these NM triggers signaling, while inhibition mitigates effects, suggesting potential therapeutic strategy.

Язык: Английский

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Chloropicrin induced ocular injury: Biomarkers, potential mechanisms, and treatments

Toxicology Letters, Год журнала: 2024, Номер 396, С. 70 - 80

Опубликована: Апрель 25, 2024

Язык: Английский

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Amelioration of Fibrosis via S1P Inhibition Is Regulated by Inactivation of TGF-β and SPL Pathways in the Human Cornea

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(12), С. 6560 - 6560

Опубликована: Июнь 14, 2024

Human corneal fibrosis can lead to opacity and ultimately partial or complete vision loss. Currently, transplantation is the only treatment for severe comes with risk of rejection donor shortages. Sphingolipids (SPLs) are known modulate in various tissues organs, including cornea. We previously reported that SPLs tightly related both, transforming growth factor beta (TGF-β) signaling fibrogenesis. The aim this study was investigate effects sphingosine-1-phosphate (S1P) S1P inhibition on specific TGF-β SPL family members fibrosis. Healthy human fibroblasts (HCFs) were isolated cultured EMEM + FBS VitC (construct medium) 3D transwells 4 weeks. following treatments prepared a construct medium: 0.1 ng/mL TGF-β1 (β1), 1 μM (S1P), 5 Sphingosine kinase inhibitor 2 (I2). Five groups tested: (1) control (no treatment); rescue groups; (2) β1/S1P; (3) β1/I2; prevention (4) S1P/β1; (5) I2/β1. Each administered weeks one switched another Using Western blot analysis, constructs examined expression fibrotic markers, SPL, pathway members. Scratch assays from 2D cultures also utilized evaluate cell migration observed reduced inactivation latent binding proteins (LTBPs), receptors, Suppressor Mothers Against Decapentaplegic homologs (SMADs), I2 compared rescue, respectively. Furthermore, we increased stimulation groups, decreased after 12 h 18 post-scratch. have demonstrated modulated LTBPs, SPLs, canonical downstream SMAD pathway. Further investigations warranted order fully uncover potential utilizing SphK as novel therapy

Язык: Английский

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Curcumin Alleviates Arecoline-induced Oral Submucous Fibrosis via the FOSL1/MAPK8 Axis

Cell Biochemistry and Biophysics, Год журнала: 2024, Номер unknown

Опубликована: Дек. 15, 2024

Язык: Английский

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PI3K Signaling and Lysyl Oxidase is Critical to Corneal Stroma Fibrosis Following Mustard Gas Injury

Experimental Eye Research, Год журнала: 2024, Номер 251, С. 110213 - 110213

Опубликована: Дек. 18, 2024

Язык: Английский

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