Advances in Plant-Derived Extracellular Vesicle Extraction Methods and Pharmacological Effects

Biology, Год журнала: 2025, Номер 14(4), С. 377 - 377

Опубликована: Апрель 6, 2025

Extracellular vesicles (EVs) are those with a double-membrane structure that contains proteins, lipids, nucleic acids, and other biologically active substances play an important role in cell–cell cell–environment communication. They have also become mechanism for exchanging cellular molecules. As many studies on EVs been conducted, plant-derived extracellular (PDEVs) started attracting attention. The biological activity stability of PDEVs closely related to the extraction separation methods, choosing method meets requirements is important. methods include ultracentrifugation, ultrafiltration, size-exclusion chromatography, etc. In recent years, it has found through research possess properties, such as anti-inflammatory, anti-cancer, anti-infective they show unique advantages therapeutic agents drug carriers. Therefore, we collected scientific literature derived from more than dozen fruits vegetables, summarized analyzed their extraction, separation, roles disease treatment, aiming provide reference inspiration in-depth study efficacy new drugs.

Язык: Английский

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Stability Dynamics of Plant-Based Extracellular Vesicles Drug Delivery

Journal of Xenobiotics, Год журнала: 2025, Номер 15(2), С. 55 - 55

Опубликована: Апрель 13, 2025

Plant-based extracellular vesicles (PBEVs) have been recognized for their wide range of applications in drug delivery however, the extent medicinal applicability depends on how well they are preserved and stored. Assessing physicochemical properties, such as size, particle concentration, shape, activity cargo, forms foundation determining stability during storage. Moreover, evaluation PBEVs is essential to ensure both safety efficacy, which critical advancing clinical development. Maintaining biological EVs storage a challenging task, similar preservation cells other cell-derived products like proteins. However, despite limited studies, it expected that storing drug-loaded may present fewer challenges compared cell-based therapies, although some limitations inevitable. This article provides comprehensive overview current knowledge methods, particularly focusing role carriers. hold promise potential candidates oral administration due effective intestinal absorption ability withstand basic acidic environments. maintaining critical. this review centers isolation, characterization, PBEVs, exploring advantages offer. Furthermore, highlights key areas require further research overcome existing enhance development methods therapeutic EVs.

Язык: Английский

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Nano-bio-encapsulation of phyto-vaccines: a breakthrough in targeted cancer immunotherapy

Molecular Biology Reports, Год журнала: 2024, Номер 52(1)

Опубликована: Дек. 18, 2024

Язык: Английский

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mRNA Technology and Mucosal Immunization

Vaccines, Год журнала: 2024, Номер 12(6), С. 670 - 670

Опубликована: Июнь 17, 2024

Current mRNA vaccines are mainly administered via intramuscular injection, which induces good systemic immunity but limited mucosal immunity. Achieving through vaccination could diminish pathogen replication at the entry site and reduce interhuman transmission. However, delivering to mucosae faces challenges like degradation, poor into cells, reactogenicity. Encapsulating in extracellular vesicles may protect reactogenicity, making possible. Plant-derived from edible fruits have been investigated as carriers. Studies animals show that vehiculated orange-derived can elicit both immune responses when by oral, nasal, or routes. Once lyophilized, these products remarkable stability. The optimization of improve translation efficiency, immunogenicity, stability be obtained adjustments 5′cap region, poly-A tail, codons selection, use nucleoside analogues. Recent studies also proposed self-amplifying RNA containing an polymerase well circular constructs. Data parenterally primed demonstrate efficacy nasal immunization with non-adjuvanted protein, humans indicate combination a parenteral vaccine natural exposure same antigen provides protection reduces Hence, would beneficial least organisms pre-treated vaccines. This practice wide applications for treatment infectious diseases.

Язык: Английский

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Mucosal mRNA Vaccines. Merits and Perspectives

Опубликована: Фев. 12, 2024

This review highlights the potential and limitations of mRNA vaccines with a particular focus on mucosal delivery as an alternative to parenteral administration. The strengths are: a) Versatility: they can be adapted target wide range pathogens; b) Faster development: designed produced more quickly than traditional vaccines; c) High efficacy: effective in preventing severe disease, demonstrated during COVID-19 pandemic. Current challenges: Delivery: current methods (mainly intramuscular injection) may not optimal for inducing immunity; Stability: is fragile needs protected from degradation; Safety: side effects, including myocarditis, blood clotting, allergic reactions need further investigation. Mucosal could transmission pathogens generally better accepted by population. Pros: offers induce local immunity at site pathogen entry, potentially providing broader protection; Cons: developing systems adjuvants difficult. Extracellular vesicles system are promising offer safe, scalable, needle-free approach. Future directions: Optimizing constructs: strategies like codon optimization self-amplifying RNA improve stability translation efficiency; Rigorous pre-clinical studies essential before human trials.

Язык: Английский

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Physical, biochemical, and biological characterization of olive-derived lipid nanovesicles for drug delivery applications

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 18, 2024

Язык: Английский

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Left out in the cold - inequity in infectious disease control due to cold chain disparity

Vaccine, Год журнала: 2024, Номер 45, С. 126648 - 126648

Опубликована: Дек. 20, 2024

Язык: Английский

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