Metabolic Profiling of Breast Cancer Cell Lines: Unique and Shared Metabolites DOI Open Access
Mariana Gallo, E Ferrari, Federica Brugnoli

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 969 - 969

Опубликована: Янв. 24, 2025

Breast Cancer (BrCa) exhibits a high phenotypic heterogeneity, leading to the emergence of aggressive clones and development drug resistance. Considering BrCa heterogeneity that metabolic reprogramming is cancer hallmark, we selected seven cell lines with diverse subtypes provide their comprehensive metabolome characterization: five commonly used (SK-Br-3, T-47D, MCF-7, MDA-MB-436, MDA-MB-231), two patient-derived xenografts (Hbcx39 Hbcx9). We characterized endometabolomes using 1H-NMR spectroscopy. found distinct metabolite profiles, certain metabolites being common but differentially accumulated across lines. High levels glycine, lactate, glutamate, formate, known promote invasion metastasis, were detected in all cells. In our experiment setting identified unique specific lines: xanthine 2-oxoglutarate SK-Br-3, 2-oxobutyrate cystathionine glucose-1-phosphate NAD+ isocitrate MDA-MB-231, NADP+ Hbcx9. The enriched enabled us identify pathways modulated cell-line-specific manner, which may represent potential candidate targets for therapeutic intervention. believe this study contribute functional characterization cells assist selecting appropriate drug-response studies.

Язык: Английский

Metabolic Profiling of Breast Cancer Cell Lines: Unique and Shared Metabolites DOI Open Access
Mariana Gallo, E Ferrari, Federica Brugnoli

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 969 - 969

Опубликована: Янв. 24, 2025

Breast Cancer (BrCa) exhibits a high phenotypic heterogeneity, leading to the emergence of aggressive clones and development drug resistance. Considering BrCa heterogeneity that metabolic reprogramming is cancer hallmark, we selected seven cell lines with diverse subtypes provide their comprehensive metabolome characterization: five commonly used (SK-Br-3, T-47D, MCF-7, MDA-MB-436, MDA-MB-231), two patient-derived xenografts (Hbcx39 Hbcx9). We characterized endometabolomes using 1H-NMR spectroscopy. found distinct metabolite profiles, certain metabolites being common but differentially accumulated across lines. High levels glycine, lactate, glutamate, formate, known promote invasion metastasis, were detected in all cells. In our experiment setting identified unique specific lines: xanthine 2-oxoglutarate SK-Br-3, 2-oxobutyrate cystathionine glucose-1-phosphate NAD+ isocitrate MDA-MB-231, NADP+ Hbcx9. The enriched enabled us identify pathways modulated cell-line-specific manner, which may represent potential candidate targets for therapeutic intervention. believe this study contribute functional characterization cells assist selecting appropriate drug-response studies.

Язык: Английский

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