Oxidative Stress and the NLRP3 Inflammasome: Focus on Female Fertility and Reproductive Health

Cells, Год журнала: 2025, Номер 14(1), С. 36 - 36

Опубликована: Янв. 2, 2025

Chronic inflammation is increasingly recognized as a critical factor in female reproductive health; influencing natural conception and the outcomes of assisted technologies such vitro fertilization (IVF). An essential component innate immunity, NLR family pyrin domain-containing 3 (NLRP3) inflammasome one major mediators inflammatory responses, its activation closely linked to oxidative stress. This interaction contributes decline oocyte quality, reduced potential, impaired embryo development. In ovarian milieu, stress NLRP3 interact intricately, their combined effects on competence are significant. The aims this review examine these molecular mechanisms explore therapeutic strategies targeting activity, with goal enhancing fertility improving clinical health.

Язык: Английский

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Antioxidant enzyme dynamics suggest the absence of oxidative stress in the canine endometrium across the estrous cycle

Animal Reproduction Science, Год журнала: 2025, Номер 275, С. 107819 - 107819

Опубликована: Март 5, 2025

Oxidative stress plays a vital role in female fertility, yet the mechanisms regulating oxidative balance canine endometrium remain poorly understood. This study investigates dynamics of antioxidant enzyme system endometrium, focusing on superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), reductase (GSR), and glutathione-S-transferase (GST), along with thiobarbituric acid reactive substances (TBARS) total cellular thiols. Enzyme activities were revealed five different phases oestrus cycle 25 dogs, using spectrophotometric method: anestrus, proestrus, estrus, early diestrus (n = 5 per group). Notably, distinctive pattern SOD CAT activity was observed, former being characterised by decrease from anestrus to later showed an opposite increase diestrus. In contrast, glutathione-dependent enzymes GPX, GSR, GST remained remarkably stable, although showing some fluctuations stages. TBARS analysis indicated evident stress-related lipid peroxidation only between proestrus. Conversely, thiol cell content consistent within Our examination ratios underscores delicate normal uterus, effectively controlling without causing damage lipids or proteins due excessive oxygen species. These findings contribute our understanding unique physiological offering valuable insights into intricate regulation this context its potential implications for fertility.

Язык: Английский

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Gut microbiota participates in polystyrene microplastics-induced defective implantation through impairing uterine receptivity

Journal of Environmental Management, Год журнала: 2025, Номер 380, С. 124997 - 124997

Опубликована: Март 17, 2025

Язык: Английский

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Identification of Candidate Genes and eQTLs Related to Porcine Reproductive Function

Animals, Год журнала: 2025, Номер 15(7), С. 1038 - 1038

Опубликована: Апрель 3, 2025

Expression quantitative trait locus (eQTL) mapping is an effective tool for identifying genetic variations that regulate gene expression. An increasing number of studies suggested SNPs associated with complex traits in farm animals are considered as expression loci. Identifying eQTLs levels the endometrium helps to unravel regulatory mechanisms genes related reproductive functions this tissue and provides molecular markers improvement high-fertility sow breeding. In study, 218 RNA-seq data from pig endometrial were used eQTL analysis identify variants regulating Additionally, weighted co-expression network (WGCNA) was performed hub involved functions. The identified 34,876 significant cis-eQTLs 5632 (FDR ≤ 0.05), 90 by WGCNA analysis. By integrating results, 14 candidate 16 fine-mapped identified, including FRK, ARMC3, SLC35F3, TMEM72, FFAR4, SOWAHA, PSPH, FMO5, HPN, FUT2, RAP1GAP, C6orf52, SEL1L3, CLGN, which physiological processes reproduction sows through hormone regulation, cell adhesion, amino acid lipid metabolism. These high endometrium, thereby affecting reproductive-related findings enhance our understanding basis provide valuable marker-assisted selection (MAS), can be applied improve fecundity optimize breeding strategies performance.

Язык: Английский

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Regulation and function of inosine monophosphate dehydrogenase 2 cytoophidia during mouse and human decidualization

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111795 - 111795

Опубликована: Апрель 1, 2025

Язык: Английский

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Activated THP-1 Macrophage-Derived Factors Increase the Cytokine, Fractalkine, and EGF Secretions, the Invasion-Related MMP Production, and Antioxidant Activity of HEC-1A Endometrium Cells

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9624 - 9624

Опубликована: Сен. 5, 2024

Endometrium receptivity is a multifactor-regulated process involving progesterone receptor-regulated signaling, cytokines and chemokines, additional growth regulatory factors. In the female reproductive system, macrophages have distinct roles in regulation of receptivity, embryo implantation, immune tolerance, angiogenesis or oxidative stress. present study, we investigated effects PMA-activated THP-1 on receptivity-related genes, regulators, stress-related molecules HEC-1A endometrium cells. We established non-contact co-culture which culture medium exhibiting pro-inflammatory phenotype was used for treatment endometrial cells, expression growth-related factors activin bone morphogenetic protein 2, hormone EGF, activation downstream signaling pERK1/2 pAkt were analyzed by ELISA Western blot. The secretions are involved establishment matrix metalloproteinases implicated invasion also determined. Based results, may play role They alter secretion as well level MMPs Moreover, activated elevate stress protection All these suggest that special implantation-related

Язык: Английский

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Impact of Hysteroscopic Polypectomy on IVF Outcomes in Women with Unexplained Infertility

Journal of Clinical Medicine, Год журнала: 2024, Номер 13(16), С. 4755 - 4755

Опубликована: Авг. 13, 2024

Objective: To assess the effect of hysteroscopic polypectomy on in vitro fertilization (IVF) results infertile women with at least one prior negative IVF outcome. Methods: This retrospective cohort study included who had attended “2nd Department Obstetrics and Gynecology National Kapodistrian University Athens” “Iaso” Maternity Hospital from October 2019 to January 2023 for infertility treatment. The medical records 345 aged 18–45 years old without abnormal findings hysterosalpingography (HSG) previous failed procedure were analyzed. male factor was excluded, as well a removal polyps. In 67 women, polyps suspected during initial two-dimensional ultrasound (2D-US) examination. final sample comprised 40 patients, which endometrial removed by hysteroscopy use resectoscope. All patients underwent ovarian stimulation consecutive cycle using short GnRh antagonist protocol. Main Results: After polypectomy, 29 (72.5%) out positive pregnancy result: 26 (65%) clinical 3 (7.5%) biochemical pregnancies documented. There statistically significant difference between number before after (p < 0.001), total 0.001). Secondary Women outcome significantly younger lower FSH levels 0.007). They also higher AMH 0.009) peak estradiol 0.013) yielded more M II oocytes embryos 0.002). Conclusions: Hysteroscopic polyp 2D history attempt improves outcomes terms pregnancies.

Язык: Английский

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Exploring the Role of Endoplasmic Reticulum Stress in Recurrent Spontaneous Abortion: Identification of Diagnostic Biomarkers and Immune Cell Interactions

Heliyon, Год журнала: 2024, Номер 10(19), С. e38964 - e38964

Опубликована: Окт. 1, 2024

Язык: Английский

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