Deciphering the Role of Cancer Stem Cells: Drivers of Tumor Evolution, Therapeutic Resistance, and Precision Medicine Strategies
Cancers,
Год журнала:
2025,
Номер
17(3), С. 382 - 382
Опубликована: Янв. 24, 2025
Cancer
stem
cells
(CSCs)
play
a
central
role
in
tumor
progression,
recurrence,
and
resistance
to
conventional
therapies,
making
them
critical
focus
oncology
research.
This
review
provides
comprehensive
analysis
of
CSC
biology,
emphasizing
their
self-renewal,
differentiation,
dynamic
interactions
with
the
microenvironment
(TME).
Key
signaling
pathways,
including
Wnt,
Notch,
Hedgehog,
are
discussed
detail
highlight
potential
as
therapeutic
targets.
Current
methodologies
for
isolating
CSCs
critically
examined,
addressing
advantages
limitations
advancing
precision
medicine.
Emerging
technologies,
such
CRISPR/Cas9
single-cell
sequencing,
explored
transformative
unraveling
heterogeneity
informing
strategies.
The
also
underscores
pivotal
TME
supporting
survival,
promoting
metastasis,
contributing
resistance.
Challenges
arising
from
CSC-driven
dormancy
analyzed,
along
strategies
mitigate
these
barriers,
novel
therapeutics
targeted
approaches.
Ethical
considerations
integration
artificial
intelligence
designing
CSC-specific
therapies
essential
elements
future
manuscript
advocates
multi-disciplinary
approach
that
combines
innovative
advanced
therapeutics,
collaborative
research
address
complexities
CSCs.
By
bridging
existing
gaps
knowledge
fostering
advancements
personalized
medicine,
this
aims
guide
development
more
effective
cancer
treatment
strategies,
ultimately
improving
patient
outcomes.
Язык: Английский
Nanoparticle troopers: Infiltrating cancer cells for targeted therapies
Nano-Structures & Nano-Objects,
Год журнала:
2025,
Номер
41, С. 101453 - 101453
Опубликована: Фев. 1, 2025
Язык: Английский
Application of network pharmacology, bioinformatics, computational molecular docking, and experimental validation to study the anticancer effects of oleanolic acid in oral squamous carcinoma cells
Acta Pharmaceutica,
Год журнала:
2025,
Номер
75(1), С. 41 - 68
Опубликована: Март 1, 2025
Abstract
Oleanolic
acid
(OA)
has
demonstrated
anticancer
effects
across
various
cancers,
with
some
derivatives
advancing
to
clinical
trials.
Howe
ver,
its
precise
mechanisms
of
action
remain
unclear,
especially
in
oral
squamous
cell
carcinoma
(OSCC).
This
study
employed
network
pharmacology,
bioinformatics,
molecular
docking,
dynamics
simulations,
and
experimental
validation
explore
OA’s
OSCC
elucidate
mechanism
action.
pharmacokinetic
physicochemical
properties
were
assessed
using
SwissADME
Molsoft,
revealing
high
bioavailability
GI
absorption.
SwissTargetPrediction
SuperPred
identified
protein
targets,
whereas
GeneCards
provided
OSCC-related
targets.
A
Venn
diagram
showed
34
overlapping
targets
between
OA
OSCC.
STRING
Cytoscape
used
construct
a
protein-protein
interaction
(PPI)
32
nodes
164
edges,
identifying
HSP90AA1,
STAT3,
HSP90AB1,
PI3KR1,
NFKB1
as
key
hub
genes.
Gene
ontology
KEGG
enrichment
analyses
highlighted
relevant
biological
processes,
functions,
pathways.
Molecular
docking
simulations
confirmed
the
strong
binding
Experimental
that
inhibited
viability
colony
formation
dose-dependent
manner,
induced
apoptosis,
downregulated
PI3KR1
proteins.
In
conclusion,
this
comprehensive
combining
assays
provides
valuable
insights
into
potential
detailed
Язык: Английский
Studies on the thermal sensitivity of lung cancer cells exposed to an alternating magnetic field and magnesium-doped maghemite nanoparticles
Cancer Nanotechnology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 22, 2024
Abstract
Background
Magnetic
fluid
hyperthermia
(MFH)
represents
a
promising
therapeutic
strategy
in
cancer
utilizing
the
heating
capabilities
of
magnetic
nanoparticles
when
exposed
to
an
alternating
field
(AMF).
Because
efficacy
and
safety
MFH
treatments
depends
on
numerous
intrinsic
extrinsic
factors,
therefore,
proper
setups
should
focus
thermal
energy
dosed
into
cells.
Methods
In
this
study,
we
performed
experiments
using
human
lung
A549
cells
(in
vitro)
NUDE
Balb/c
mice
bearing
(A549)
vivo).
these
two
experimental
models,
heat
was
induced
by
magnesium-doped
iron(III)
oxide
coated
with
mPEG-silane
(Mg
0.1
-γ-Fe
2
O
3
(mPEG-silane)
0.5
)
AMF.
Results
We
observed
that
treated
Mg
(0.25
mg·mL
−1
magnetized
for
30
min
at
14.4
kA·m
yielded
satisfactory
outcome
reducing
cell
viability
up
ca.
21%
vitro).
The
activation
calculated
strength
estimated
349
kJ·mol
.
Both
volumetric
measurements
tumor
mass
assessments
confirmed
resonance
imaging
(MRI)
showed
superior
effect
injected
intratumorally
(3
subjected
AMF
(18.3
four
times
weekly
intervals.
Research
demonstrated
undergoing
exhibited
marked
suppression
growth
(V
=
169
±
94
mm
;
p
<
0.05)
comparison
control
group
untreated
mice.
CEM43
(cumulative
number
equivalent
minutes
43
°C)
value
were
9.6
specific
absorption
rate
(SAR)
level
ranging
from
100
150
W·g
Conclusions
as-obtained
results,
both
cytotoxic
those
related
calculations
SAR,
may
contribute
advancement
therapies,
concurrently
indicating
proposed
holds
great
potential
further
testing
context
medical
applications.
Graphical
Язык: Английский