Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor

Biosensors, Год журнала: 2025, Номер 15(1), С. 58 - 58

Опубликована: Янв. 17, 2025

MicroRNA122 (miR-122) is a microRNA that highly expressed in hepatocytes and has been identified as prospective therapeutic target biomarker for liver injury. An expanding body of research demonstrated miR-122 critical regulator both the initiation progression wide range diseases. Traditional methods detecting mainly include Northern blotting qRT-PCR, but they are technically complex cumbersome, requiring expensive instruments high technical requirements. In this paper, we present novel rapid testing method utilizing lateral flow nucleic acid biosensor (LFNAB) sensitive time-efficient detection miR-122. This approach offers several advantages, including specificity miR-122, ability to detect low concentrations molecule, significantly reduced time compared conventional methods. study, thiol-modified single-stranded DNA probe (Det-DNA), biotinylated capture (Cap-DNA), control (Con-DNA) used construct LFNAB. A gold nanoparticle (AuNP) colored tag, which label Det-DNA probe. The principle based on dual DNA-miRNA hybridization reactions LFNAB form sandwich-type AuNP-Det-DNA-miR-122-Cap-DNA complexes, captured test area visualization quantification. After systematic optimization conditions experiment, response was linear within scope 0 pM-100 pM limit 15 min 3.90 pM. use serum fingertip blood yielded satisfactory results. successful application indicates effectiveness samples, showcasing its potential utility clinical settings assessing levels different biological samples.

Язык: Английский

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miR-30d Levels Predict Re-Hospitalization in Patients with Acute Cardiogenic Pulmonary Edema: A Preliminary Study

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1278 - 1278

Опубликована: Фев. 1, 2025

Acute cardiogenic pulmonary edema (ACPE) is a common and serious manifestation of heart failure (HF), representing 10-20% all acute HF admissions. It associated with elevated in-hospital mortality high rates re-hospitalization. MicroRs, like miR-30d, are particular interest in due to their regulatory role gene expression potential as biomarkers for diagnosing predicting patient outcomes, especially high-risk cases such ACPE. We conducted cohort study on patients diagnosed ACPE the Emergency Department (ED). The circulating levels miR-30d were analyzed at time hospital admission one-month follow-up along other biomarkers. enrolled 24 10 control subjects. Median age was 80.8 years (interquartile range, IQR, 8.2) cases, 78.5 (IQR 9.8) controls male/female ratio 2 0.66, respectively. In patients, median cardiac ejection fraction 42.5%, creatinine 1.63 mg/dL 1.24), troponin 63.5 ng/dL (58), NT-proBNP 4243.5 pg/mL 5846) ED evaluation. concentration miR30d 0.81 controls, 3.67 7.28 enrollment one month later, Re-hospitalization occurred 7 following 3 months, 9 during year. had significant predictive value assessing risk re-hospitalization both months 1 year initial diagnosis ACPE, while it did not death When compared biomarkers, none them showed better accuracy than miR-30d. Our findings suggest that an increased rate readmission after discharge. Larger, multicenter studies will be needed confirm validity biomarker useful prediction its utility improving individualized care.

Язык: Английский

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A novel magnetic fluorescent nanoprobe for simultaneous separation and identification of microRNA199a in breast cancer

Biochemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 109676 - 109676

Опубликована: Фев. 1, 2025

Язык: Английский

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Epigenetic insights of microRNAs: Pioneering cancer detection via advanced biosensors

International review of cell and molecular biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Single-Step Detection of microRNA via the Programmable DNAzyme Probe-Mediated Multiple Signal Recycling

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

The significant role of microRNAs (miRNAs) in the regulation pathological ocular neovascularization makes them both biomarkers and therapeutic targets vascular eye diseases. However, their acute sensitive detection continue to be a challenge. An integrative DNA probe (detection probe) was developed enable single-step miRNAs with high sensitivity specificity. This combines functions specific target recognition, DNAzyme unit-based signal reaction, polymerase/endonuclease-assisted cycle. In particular, single-stranded sequence that engages unit s2 can identify miRNA liberate facilitate reaction help metal ions. addition, recycling process cycle were initiated by chain extension displacement process, resulting low limit 4.56 fM. method demonstrated level septicity for capable distinguishing interfering one-base mismatch. Our research has shown nanomachine is promising biosensor single step. It anticipated this technology will used early diagnosis therapy

Язык: Английский

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Identifying gene expression predictive of response to neoadjuvant endocrine therapy in early breast cancer

Breast Cancer Research and Treatment, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Abstract Purpose Estrogen receptor (ER)-positive breast cancer is the most common subtype, accounting for approximately 80% of cases, with endocrine therapy as standard postoperative treatment. However, despite risk-reducing therapies, risk recurrence remains substantial. Studies, including POETIC trial, have demonstrated that low Ki67 levels following short-term neoadjuvant (sNAET) are associated a favorable prognosis. The objective this study to identify genes suppression cell cycle progression by sNAET in postmenopausal patients ER-positive/human epidermal growth factor 2-negative cancer. Methods Ninety-seven tissue samples were collected and classified into groups based on expression before after RNA sequencing real-time quantitative reverse transcription PCR performed analyze gene tumor from stratified High–High (H–H) or High–Low (H–L) sNAET. Results Among differentially expressed identified, CXCL9 ABCA12 significantly upregulated H–H group poor response therapy. Conversely, NPY1R was H–L group, suggesting greater responsiveness. In multivariate logistic regression analysis, (OR: 0.65, p = 0.024) 1.61, 0.048) significant predictors reduction. Conclusion These findings suggest could serve predictive biomarkers response. Identifying these may facilitate personalized treatment strategies, addition therapies such chemotherapy resistant cases.

Язык: Английский

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Recent advances in nanotechnology-based diagnosis and treatment of bladder cancer: an updated review

International Journal of Smart and Nano Materials, Год журнала: 2025, Номер unknown, С. 1 - 34

Опубликована: Апрель 19, 2025

Язык: Английский

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MicroRNA Nobel Prize: Timely Recognition and High Anticipation of Future Products—A Prospective Analysis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12883 - 12883

Опубликована: Ноя. 29, 2024

MicroRNAs (miRNAs) maintain cellular homeostasis by blocking mRNAs binding with them to fine-tune the expression of genes across numerous biological pathways. The 2024 Nobel Prize in Medicine and Physiology for discovering miRNAs was long overdue. We anticipate a deluge research work involving repeat history prizes awarded on other RNAs. Although miRNA therapies are included several complex diseases, realization that regulate their roles addressing hundreds diseases expected; but advancement drug discovery tools, we even faster entry new drugs. To promote this, provide details current science, logic, intellectual property, formulations, regulatory process anticipation many more researchers will introduce novel based discussion advice provided this paper.

Язык: Английский

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MicroRNA signatures in neuroplasticity, neuroinflammation and neurotransmission in association with depression

Journal of Physiology and Biochemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

Язык: Английский

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A novel and sensitive trefoil-structured biosensor based on nanoporous gold for simultaneous determination of microRNA-21 and microRNA-16

Biosensors and Bioelectronics, Год журнала: 2024, Номер 272, С. 117093 - 117093

Опубликована: Дек. 27, 2024

Язык: Английский

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