Evaluation of the neuroprotective potential of benzylidene digoxin 15 against oxidative stress in a neuroinflammation models induced by lipopolysaccharide and on neuronal differentiation of hippocampal neural precursor cells DOI Creative Commons
Gláucia Cordeiro,

Jéssica Alves Faria,

L.H.S. Pavan

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 14, 2025

Neuroinflammation, often driven by the overproduction of reactive oxygen species (ROS), plays a crucial role in pathogenesis neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. The susceptibility brain to oxidative stress is attributed its high metabolic activity limited antioxidant defense. This study aimed evaluate neuroprotective potential Benzylidene Digoxin 15 (BD-15) following treatment pretreatment lipopolysaccharide (LPS)-induced neuroinflammation model. Additionally, we examined whether BD-15 enhances generation neurons from neural progenitor cells (NPCs).Male Wistar rats were used for acute studies divided into four groups: control (saline), (100 μg/kg), LPS (250 + μg/kg 100 μg/kg). Swiss albino mice chronic (0.56 mg/kg), (1 mg/kg 0.56 mg/kg). Behavioral changes assessed using open field test, tissues analyzed markers, including malondialdehyde (MDA), reduced glutathione (GSH), protein carbonylation, catalase (CAT), superoxide dismutase (SOD), S-transferase (GST). To assess neurogenesis, primary NPC cultures derived hippocampus newborn used, which led locomotor increased stress, particularly cortex, indicated elevated MDA levels GSH levels. reversed these effects, notably restoring reducing carbonylation cerebellum. Chronic improved markers MDA, SOD, CAT, GST. Furthermore, exhibits properties alleviating motor dysfunction, suggesting therapeutic agent neuroinflammatory disorders. However, did not affect cell proliferation, indicating that this cardiotonic steroid alter cycle cells.

Язык: Английский

Evaluation of the Anti-Mycobacterial and Anti-Inflammatory Activities of the New Cardiotonic Steroid γ-Benzylidene Digoxin-15 in Macrophage Models of Infection DOI Creative Commons
Daniel Magalhães,

Maria Gabriella S. Sidrônio,

N.F.S. Nogueira

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 269 - 269

Опубликована: Янв. 25, 2025

Cardiotonic steroids modulate various aspects of the inflammatory response. The synthetic cardiotonic steroid γ-benzylidene digoxin 15 (BD-15), a derivative, has emerged as promising candidate with potential immunomodulatory effects. However, its biological activity remains largely unexplored. This study investigated anti-mycobacterial and anti-inflammatory effects BD-15 in an vitro macrophage infection model Mycobacterium spp. Unlike digoxin, which showed significant toxicity at higher concentrations, exhibited no cytotoxicity RAW 264.7 cells (a murine cell line). Both compounds were evaluated smegmatis-infected cells, reducing bacterial burden without direct bactericidal activity. Additionally, both modulated pro-inflammatory cytokine levels, notably by decreasing tumor necrosis factor alpha (TNF-α) interleukin-1 beta (IL-1β) levels. specifically reduced NOD-, LRR-, pyrin-domain-containing protein 3 (NLRP3) inflammasome expression increased interleukin-10 (IL-10) production. Notably, colony-forming unit (CFU) counts tuberculosis-infected cells. Toxicity assays HepG2 human liver cancer line) that had minimal hepatotoxicity compared to demonstrated negligible acute Artemia salina bioassay. These findings revealed highlighted safer alternative for therapeutic applications.

Язык: Английский

Процитировано

2
Evaluation of the neuroprotective potential of benzylidene digoxin 15 against oxidative stress in a neuroinflammation models induced by lipopolysaccharide and on neuronal differentiation of hippocampal neural precursor cells DOI Creative Commons
Gláucia Cordeiro,

Jéssica Alves Faria,

L.H.S. Pavan

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 14, 2025

Neuroinflammation, often driven by the overproduction of reactive oxygen species (ROS), plays a crucial role in pathogenesis neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. The susceptibility brain to oxidative stress is attributed its high metabolic activity limited antioxidant defense. This study aimed evaluate neuroprotective potential Benzylidene Digoxin 15 (BD-15) following treatment pretreatment lipopolysaccharide (LPS)-induced neuroinflammation model. Additionally, we examined whether BD-15 enhances generation neurons from neural progenitor cells (NPCs).Male Wistar rats were used for acute studies divided into four groups: control (saline), (100 μg/kg), LPS (250 + μg/kg 100 μg/kg). Swiss albino mice chronic (0.56 mg/kg), (1 mg/kg 0.56 mg/kg). Behavioral changes assessed using open field test, tissues analyzed markers, including malondialdehyde (MDA), reduced glutathione (GSH), protein carbonylation, catalase (CAT), superoxide dismutase (SOD), S-transferase (GST). To assess neurogenesis, primary NPC cultures derived hippocampus newborn used, which led locomotor increased stress, particularly cortex, indicated elevated MDA levels GSH levels. reversed these effects, notably restoring reducing carbonylation cerebellum. Chronic improved markers MDA, SOD, CAT, GST. Furthermore, exhibits properties alleviating motor dysfunction, suggesting therapeutic agent neuroinflammatory disorders. However, did not affect cell proliferation, indicating that this cardiotonic steroid alter cycle cells.

Язык: Английский

Процитировано

0