Mass Spectrometry–Based Proteomics in Clinical Diagnosis of Amyloidosis and Multiple Myeloma: A Review (2012–2024) DOI Creative Commons

Katerina Kratka,

Pavel Šištík,

Ivana Olivkova

и другие.

Journal of Mass Spectrometry, Год журнала: 2025, Номер 60(3)

Опубликована: Фев. 19, 2025

ABSTRACT Proteomics is nowadays increasingly becoming part of the routine clinical practice diagnostic laboratories, especially due to advent advanced mass spectrometry techniques. This review focuses on application proteomic analysis in identification pathological conditions a hospital setting, with particular focus protein biomarkers. In particular, main purpose highlight challenges associated specific disease‐causing proteins, given their complex nature and variety posttranslational modifications (PTMs) they can undergo. PTMs, such as phosphorylation glycosylation, play critical roles function but also lead diseases if dysregulated. plays an important role various medical fields ranging from cardiology, internal medicine hemato‐oncology emphasizing interdisciplinary this field. Traditional methods electrophoretic or immunochemical have been mainstay detection; however, these techniques are limited terms specificity sensitivity. Examples include diagnosis multiple myeloma detection its amyloidosis, which relies heavily conventional methods, sometimes false positives inadequate disease monitoring. Mass respect emerges superior alternative, providing high sensitivity quantification sequences. technique particularly beneficial for monitoring minimal residual (MRD) where traditional fall short. Furthermore provide precise typing amyloid crucial appropriate treatment amyloidosis. summarizes opportunities determination using between 2012 2024, highlighting transformative potential proteomics encouraging wider use laboratories.

Язык: Английский

Mass Spectrometry–Based Proteomics in Clinical Diagnosis of Amyloidosis and Multiple Myeloma: A Review (2012–2024) DOI Creative Commons

Katerina Kratka,

Pavel Šištík,

Ivana Olivkova

и другие.

Journal of Mass Spectrometry, Год журнала: 2025, Номер 60(3)

Опубликована: Фев. 19, 2025

ABSTRACT Proteomics is nowadays increasingly becoming part of the routine clinical practice diagnostic laboratories, especially due to advent advanced mass spectrometry techniques. This review focuses on application proteomic analysis in identification pathological conditions a hospital setting, with particular focus protein biomarkers. In particular, main purpose highlight challenges associated specific disease‐causing proteins, given their complex nature and variety posttranslational modifications (PTMs) they can undergo. PTMs, such as phosphorylation glycosylation, play critical roles function but also lead diseases if dysregulated. plays an important role various medical fields ranging from cardiology, internal medicine hemato‐oncology emphasizing interdisciplinary this field. Traditional methods electrophoretic or immunochemical have been mainstay detection; however, these techniques are limited terms specificity sensitivity. Examples include diagnosis multiple myeloma detection its amyloidosis, which relies heavily conventional methods, sometimes false positives inadequate disease monitoring. Mass respect emerges superior alternative, providing high sensitivity quantification sequences. technique particularly beneficial for monitoring minimal residual (MRD) where traditional fall short. Furthermore provide precise typing amyloid crucial appropriate treatment amyloidosis. summarizes opportunities determination using between 2012 2024, highlighting transformative potential proteomics encouraging wider use laboratories.

Язык: Английский

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