Enhancing the Therapeutic Potential of Human Umbilical Cord Mesenchymal Stem Cells for Osteoarthritis: The Role of Platelet-Rich Plasma and Extracellular Vesicles DOI Open Access

Yu‐Hsun Chang,

Kun‐Chi Wu, Dah‐Ching Ding

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3785 - 3785

Опубликована: Апрель 17, 2025

Osteoarthritis (OA) is a chronic degenerative joint disease. Our previous study demonstrated that extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stem cells (HUCMSCs), which play crucial role in regenerative medicine, have therapeutic effects on OA. Additionally, platelet-rich plasma (PRP) has been widely used musculoskeletal diseases as it promotes wound healing, angiogenesis, and tissue remodeling; however, its efficacy stand-alone therapy remains controversial. Therefore, we investigated the of combining cell-derived EVs with PRP an OA model. HUCMSC-derived treated were experimental group, whereas cultured serum-free (SF) or exosome-depleted fetal bovine serum (exo(-)FBS) served controls. PRP-treated HUCMSCs maintained their surface antigen characteristics potential to differentiate into adipocytes, osteoblasts, chondrocytes. In model, mice + 5% PRP-derived showed significantly improved motor function compared controls comparable those +SF +exo(-)FBS-derived EVs. increased type II collagen aggrecan decreased IL-1β expression observed cartilage transplanted various conclusion, enhances HUCMSC differentiation, treatment improves outcomes, providing promising strategy for future clinical applications.

Язык: Английский

Enhancing the Therapeutic Potential of Human Umbilical Cord Mesenchymal Stem Cells for Osteoarthritis: The Role of Platelet-Rich Plasma and Extracellular Vesicles DOI Open Access

Yu‐Hsun Chang,

Kun‐Chi Wu, Dah‐Ching Ding

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3785 - 3785

Опубликована: Апрель 17, 2025

Osteoarthritis (OA) is a chronic degenerative joint disease. Our previous study demonstrated that extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stem cells (HUCMSCs), which play crucial role in regenerative medicine, have therapeutic effects on OA. Additionally, platelet-rich plasma (PRP) has been widely used musculoskeletal diseases as it promotes wound healing, angiogenesis, and tissue remodeling; however, its efficacy stand-alone therapy remains controversial. Therefore, we investigated the of combining cell-derived EVs with PRP an OA model. HUCMSC-derived treated were experimental group, whereas cultured serum-free (SF) or exosome-depleted fetal bovine serum (exo(-)FBS) served controls. PRP-treated HUCMSCs maintained their surface antigen characteristics potential to differentiate into adipocytes, osteoblasts, chondrocytes. In model, mice + 5% PRP-derived showed significantly improved motor function compared controls comparable those +SF +exo(-)FBS-derived EVs. increased type II collagen aggrecan decreased IL-1β expression observed cartilage transplanted various conclusion, enhances HUCMSC differentiation, treatment improves outcomes, providing promising strategy for future clinical applications.

Язык: Английский

Процитировано

0