Inflammopharmacology, Год журнала: 2025, Номер unknown
Опубликована: Май 31, 2025
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4472 - 4472
Опубликована: Май 8, 2025
Endometrial cancer (EC) is classified into two main subtypes with distinct molecular profiles. Sphingolipids, particularly ceramide and sphingosine-1-phosphate (S1P), are crucial regulators of cell survival, apoptosis, oxidative stress. This study examined the impact sphingolipid metabolism in Ishikawa (type 1) HEC-1A 2) EC cells following silencing Sptlc1 Sptlc2, which encode subunits serine palmitoyltransferase (SPT), a key enzyme de novo synthesis. Gene was confirmed by RT-PCR Western blot, while levels were quantified using UHPLC/MS/MS rheostat (S1P/ceramide ratio) calculated. Cell viability (MTT assay), death, ROS (ELISA), total antioxidant capacity (TAC), catalase caspase-3 activity, mitochondrial membrane potential also assessed. The obtained data showed higher Ishikawa(CON) S1P HEC-1A(CON) cells, resulting cells. SPT knockdown reduced levels, increased viability, elevated decreased Furthermore, after gene silencing, these exhibited activity diminished TAC, indicating an impaired redox balance. These findings reveal subtype-specific responses to disrupted synthesis highlight importance homeostasis behavior
Язык: Английский
Процитировано
0
Antioxidants, Год журнала: 2025, Номер 14(5), С. 578 - 578
Опубликована: Май 11, 2025
Most DNA damage caused by oxidative metabolism consists of single lesions that can accumulate in tissues. This review focuses on two classes lesions: the 8-oxopurine (8-oxo-Pu) are repaired base excision repair (BER) enzyme and four 5',8-cyclopurine (cPu) exclusively nucleotide (NER) enzyme. The aim is to correlate simultaneous quantification these context neurological disorders. first half a summary reactive oxygen species (ROS) with particular attention pathways hydroxyl radical (HO•) formation, followed protocols for six biomimetic chemistry HO• double-stranded oligonucleotides (ds-ODN) calf thymus (ct-DNA). second addresses neurodegenerative diseases: xeroderma pigmentosum (XP) Cockayne syndrome (CS). quantitative data obtained from genomic and/or mitochondrial extracts across several XP CS cell lines discussed. Oxidative stress contributes resulting accumulation cPu 8-oxo-Pu DNA. formation postulated culprit inducing symptoms associated CS.
Язык: Английский
Процитировано
0
Nutrients, Год журнала: 2025, Номер 17(11), С. 1852 - 1852
Опубликована: Май 29, 2025
“Sola dosis facit venenum” (Paracelsus). Essential trace elements, crucial for maintaining neuronal function, have their dysregulation increasingly correlated with neurodegenerative disorders, particularly Parkinson’s disease (PD). This systematic review aims to synthesize recent high-quality evidence regarding the involvement of essential such as iron, zinc, copper, manganese, and selenium, in pathogenesis and, consequently, potential therapeutic targets PD. A comprehensive literature search was conducted articles published between 1 January 2023 31 December 2024. Out an initial pool 1231 identified studies, 63 met methodological eligibility criteria according PRISMA (Preferred Reporting Items Systematic Reviews Meta-Analyses) guidelines. All potentially eligible interventional observational studies were initially assessed using Physiotherapy Evidence Database (PEDro) scale, which is commonly employed evaluating internal validity statistical interpretability clinical trials rehabilitation-focused studies. Following qualitative assessment PEDro 18 ultimately selected based on scientific relevance rigor. To supplement scoring, designed primarily individual trials, we applied AMSTAR-2 (A MeaSurement Tool Assess Reviews) checklist evaluation included reviews or meta-analyses. The a variety clinical, postmortem, experimental models investigate trace-element concentrations mechanistic roles findings revealed consistent patterns iron accumulation substantia nigra, zinc’s bidirectional effects oxidative stress autophagy, copper-induced α-synuclein aggregation, neuroprotective role selenium via antioxidant pathways. Manganese associated mitochondrial dysfunction neuroinflammation. disturbances contribute PD pathology through interconnected mechanisms involving redox imbalance, protein misfolding, impaired cellular homeostasis. These elements may serve both biomarkers tools, warranting further investigation into personalized metal-based interventions
Язык: Английский
Процитировано
0
Antioxidants, Год журнала: 2025, Номер 14(6), С. 656 - 656
Опубликована: Май 29, 2025
Redox imbalance plays a pivotal role in the regulation of inflammation, influencing both onset and progression various inflammatory conditions. While pro-inflammatory oxidative stress (OS) is well established, impact reductive (RS)—a condition marked by excessive reducing equivalents such as NADH, NADPH, reduced glutathione (GSH)—remains underappreciated. This review offers novel integrative perspective analyzing how OS RS act not merely opposition, but interconnected modulators immune function. We explore mechanisms through which activates pathways, RS, when sustained, can paradoxically impair defense, alter redox-sensitive signaling, contribute to disease progression. Emphasis placed on dynamic interplay between these redox extremes their combined contribution pathogenesis chronic diseases, including autoimmune, cardiovascular, neuroinflammatory disorders. Additionally, we evaluate therapeutic strategies that target homeostasis, arguing for shift from antioxidant-centric treatments approaches consider bidirectional nature dysregulation. framework may inform development more precise interventions inflammation-related diseases.
Язык: Английский
Процитировано
0
Inflammopharmacology, Год журнала: 2025, Номер unknown
Опубликована: Май 31, 2025
Язык: Английский
Процитировано
0