BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)
Опубликована: Дек. 30, 2024
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Язык: Английский
Molecular Genetics Microbiology and Virology (Russian), Год журнала: 2025, Номер 43(1), С. 24 - 24
Опубликована: Апрель 10, 2025
The aim. aim of this work is to evaluate the expression circRNA circLDLR, circSPARC, circSMARCA5 and circTMEM181, mediated targets which are mRNAs genes associated with atherogenesis. This study may provide updated information on involvement atherogenesis in pathogenesis coronary heart disease, as well suggest possible prospects for their use biomarkers therapeutic treatment cardiovascular diseases. Material methods. included 68 patients artery stenosis 41 control without atherosclerosis confirmed by angiography. Using real-time PCR, we measured levels circSMARCA5, circTMEM181 RNA preparations isolated from peripheral blood mononuclear cells. Results discussion. An increase circSPARC disease compared confirms pro-atherogenic effect. Conclusion. Has been suggested potential significance diagnosis atherosclerosis.
Язык: Английский
Процитировано
0
Current Atherosclerosis Reports, Год журнала: 2025, Номер 27(1)
Опубликована: Июнь 3, 2025
Circular RNAs (circRNAs), a distinct class of long noncoding characterized by covalently closed-loop structures, have emerged as pivotal regulators gene expression. Their stability, abundance, and cell-type specificity make them increasingly relevant in cardiovascular disease pathogenesis clinical management. Atherosclerosis, chronic inflammatory disorder the arterial wall, underlies many cerebrovascular events, including myocardial infarction stroke. This review provides comprehensive analysis circRNAs' influence on development progression atherosclerotic plaques. The role circRNAs atherogenesis, where they may function atheroprotective or atherogenic factors modulating endothelial smooth muscle cell functions, macrophage activity, lipid metabolism, signaling, has recently emerged. explores both experimental vivo findings functions specific their involvement cellular autophagy, apoptosis, oxidative stress, vascular remodeling. Additionally, diagnostic potential circulating biomarkers for plaque instability rupture been investigated. Understanding circRNA-mediated regulatory networks open new avenues precision diagnostics targeted therapies disease.
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2024, Номер 12(5), С. 1071 - 1071
Опубликована: Май 12, 2024
Hypertension (HT) is a disease that poses serious threat to human health, mediating organ damage such as the cardiovascular (CV) system, kidneys, central nervous system (CNS), and retinae, ultimately increasing risk of death due entire vascular system. Thus, widespread prevalence hypertension brings enormous health problems socioeconomic burdens worldwide. The goal management prevent hypertension-mediated excess mortality diseases. To achieve this goal, guidelines recommend accurate monitoring blood pressure assessment associated target damage. Early identification mediated by therefore crucial. Plasma biomarkers non-invasive test can help identify patients with who will benefit from antihypertensive treatment optimization improved prognosis. In review, we provide an overview some currently available, under-researched, potential plasma hypertension, looking for be detected simple testing hypertensive damage, which great significance in clinical work. Natriuretic peptides (NPs) utilized traditional biomarker detect especially heart failure. Nevertheless, additionally may need combine two or more monitor early stages hypertension.
Язык: Английский
Процитировано
2
BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)
Опубликована: Июнь 17, 2024
Abstract Background The role of circular RNAs (circRNAs) as biomarkers coronary artery disease (CAD) remains poorly explored. This study aimed to identify and validate potential circulating circRNAs for the diagnosis CAD. Methods expression profile associated with CAD was obtained from Gene Expression Omnibus (GEO) database. Differential analysis, weighted gene co-expression network analysis (WGCNA) least absolute shrinkage selection operation (LASSO) were employed CAD-related hub circRNAs. levels these validated using qRT-PCR in blood samples 100 patients controls. diagnostic performance evaluated through logistic regression receiver operator characteristic (ROC) integrated discrimination improvement (IDI), net reclassification (NRI). Functional enrichment analyses performed predict possible mechanisms Results A total ten identified WGCNA LASSO analysis. Among them, hsa_circ_0069972 hsa_circ_0021509 highly expressed patients, they independent predictors after adjustment relevant confounders. area under ROC curve 0.760 0.717, respectively. classification improved incorporation into clinical model composed conventional cardiovascular risk factors, showing an IDI 0.131 NRI 0.170 hsa_circ_0069972, 0.111 0.150 hsa_circ_0021509. revealed that hsa_circ_0069972-miRNA-mRNA enriched TGF-β 、 FoxO Hippo signaling pathways, while hsa_circ_0021509-miRNA-mRNA PI3K/Akt MAPK pathways. Conclusion Hsa_circ_0069972 by are patients. They may serve novel
Язык: Английский
Процитировано
2
JACC Basic to Translational Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Circular RNAs are associated with cardiovascular disease, including coronary artery but the mechanisms have not been completely elucidated. We found a new protein, circBTBD7-420aa, encoded by hsa_circ_0000563. Our results suggest that circBTBD7-420aa may inhibit abnormal proliferation and migration of human smooth muscle cells promoting SLC3A2 degradation through ubiquitin-proteasome pathway. In addition, we constructed engineered exosomes loaded can target vascular modifying peptide fragments targeting osteopontin. This study suggests progression atherosclerosis serve as for diagnosis treatment disease.
Язык: Английский
Процитировано
1
Journal of Thoracic Disease, Год журнала: 2023, Номер 15(11), С. 5971 - 5991
Опубликована: Ноя. 1, 2023
Calcific aortic valve disease (CAVD) is a common cardiovascular with high morbidity and mortality, no effective prevention or treatment available. In recent years, increasing evidence has shown that noncoding RNAs (ncRNAs) play an important role in the pathogenesis prognosis of CAVD. Several associated circular (circRNAs) have been reported to be involved CAVD, such as circRIC3 TGFBR2. However, limited number circRNAs identified CAVD warrants further in-depth investigation, comprehensive elucidation their key mechanisms this needed.The expression microRNAs (miRNAs) were analyzed by RNA sequencing. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted analyze circRNA ARHGAP10 (circARHGAP10), miR-335-3p, RUNX2. Luciferase reporter assay, pull-down binding protein immunoprecipitation (RIP) assay performed evaluate miR-335-3p circARHGAP10 Alizarin red S staining showed formation calcified nodules interstitial cells (VICs). The altered through lentivirus infection. Alkaline phosphatase (ALP) activity used verify correlation between miR-335-3p. proteins assessed via Western blot. fluorescence situ hybridization (FISH) confirm localization cytoplasm VICs. Immunofluorescence detect level ApoE-/- mice construct model, short hairpin (shRNA) inhibitor intraperitoneally injected, scramble normal control (NC) injected controls, followed hematoxylin eosin (HE) staining.Through sequencing, we found (hsa_circ_0008975) highly expressed calcific valves. CircARHGAP10 knockdown effectively inhibited extent osteogenic differentiation We then competing endogenous (ceRNA) miR-355-3p targeted vitro experiments confirmed regulated VICs miR-355-3p/RUNX2 pathway, validated vivo using mouse model.These findings provide foundation for circRNA-directed diagnostics therapeutics
Язык: Английский
Процитировано
1
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Март 21, 2024
Язык: Английский
Процитировано
0
Toxicology and Applied Pharmacology, Год журнала: 2024, Номер 492, С. 117121 - 117121
Опубликована: Окт. 9, 2024
Язык: Английский
Процитировано
0
Nanobiotechnology Reports, Год журнала: 2024, Номер 19(3), С. 381 - 392
Опубликована: Июнь 1, 2024
Язык: Английский
Процитировано
0
International Journal of Molecular Medicine, Год журнала: 2024, Номер 55(1)
Опубликована: Окт. 31, 2024
Coronary heart disease (CHD) remains a leading cause of morbidity and mortality worldwide, posing substantial public health burden. Despite advancements in treatment, the complex etiology CHD necessitates ongoing exploration novel diagnostic markers therapeutic targets. Circular RNAs (circRNAs), distinct class non‑coding with covalently closed loop structure, have emerged as significant regulators various diseases, including CHD. Their high stability, tissue‑specific expression evolutionary conservation underscore their potential biomarkers agents This review discusses current knowledge on circRNAs context explores molecular mechanisms by which influence pathophysiology CHD, cardiomyocyte death, endothelial injury, vascular dysfunction inflammation. It also summarizes emerging evidence highlighting differential patients utilities non‑invasive prognostic targets for this disease.
Язык: Английский
Процитировано
0