Proteome Reprogramming and Acquired Stress Tolerance in Potato Cells Exposed to Acute or Stepwise Water Deficit DOI Creative Commons

Elisa Cappetta,

Carmine Del Regno, Sara Ceccacci

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

ABSTRACT Water deficit negatively impacts crop productivity and quality. Plants face these challenges by adjusting biological processes molecular functions according to the intensity duration of stress. The cultivated potato ( Solanum tuberosum ) is considered sensitive water deficit, thus breeding efforts are needed enhance its resilience. To capture novel functional information gene regulatory networks, we carried out mass spectrometry‐based proteomics in cell suspensions exposed abrupt or stepwise osmotic stresses. Both forms stress triggered significant alterations protein expression, though with divergent response mechanisms. Stress pathways orchestrated key proteins enrolled primary secondary metabolism, antioxidant processes, transcriptional translational machinery chromatin organization were found adapted cells. Target metabolites reactive oxygen species levels quantified associate outcomes proteome study. Remarkably, also showed that cells tolerate an array diverse conditions, including anoxia, salt heat Finally, expression patterns genes encoding selected differentially expressed investigated plants subjected either drought Collectively, our findings reveal complex cellular strategies adaptation, identifying new fundamental could

Язык: Английский

Dynamic interplay of reactive oxygen and nitrogen species (ROS and RNS) in plant resilience: unveiling the signaling pathways and metabolic responses to biotic and abiotic stresses DOI

Rekha Thiruvengadam,

Baskar Venkidasamy, Maheswaran Easwaran

и другие.

Plant Cell Reports, Год журнала: 2024, Номер 43(8)

Опубликована: Июль 18, 2024

Язык: Английский

Процитировано

24

The Role of Dietary Phenolic Compounds in the Prevention and Treatment of Rheumatoid Arthritis: Current Reports DOI Open Access
Ana C. Gonçalves, S. Sofia M. Rodrigues,

Rafael Rodrigues

и другие.

Опубликована: Апрель 8, 2024

Rheumatoid arthritis (RA) is a complex illness with both hereditary and environmental compo-nents. Globally, in 2019, 18 million people had RA. RA characterised by persistent inflammation of the synovial membrane that lines joints, cartilage loss, bone erosion. Phenolic molecules are most prevalent secondary metabolites plants, diverse spectrum biological ac-tions benefit functional meals nutraceuticals. These compounds have received lot at-tention recently because they antioxidant, anti-inflammatory, immunomodulatory, anti-rheumatoid activity modulating tumour necrosis factor, mitogen-activated protein kinase, nu-clear factor kappa-light-chain-enhancer activated B cells, c-Jun N-terminal kinases, as well other preventative properties. This article discusses dietary polyphenols, their pharmacological properties, innovative delivery technologies for treatment RA, focus on possible activities. Nonetheless, commercialization polyphenols may be achievable only after confirming safety profile completing successful clinical trials.

Язык: Английский

Процитировано

5

Metabolites in Cellular Leachate as Biomarkers for the Nature and Severity of Injury Following a Short vs. Prolonged Freezing Stress DOI
Rajeev Arora, Keting Chen

Physiologia Plantarum, Год журнала: 2025, Номер 177(3)

Опубликована: Май 1, 2025

ABSTRACT Freeze injury is typically quantified by measuring electrolyte leakage from plant tissues exposed to a freeze–thaw cycle, whereby higher indicates greater injury. The severity of stress also assessed the ability (or lack thereof) injured tissue recover, wherein reduced during post‐thaw period relative right after thaw recoverable In this study, spinach leaves were various degrees freezing them for different durations at fixed sub‐zero temperature. We explored cellular nature comparing profiles metabolites that leaked reversibly and/or irreversibly leaves. A total 47 were, thus, detected in leachate, including 8 sugars, 20 amino acids, GABA, uracil, adenine, palmitic acid, ferulic, coumaric and other organic/inorganic acids involved primary secondary metabolism (e.g., citric phosphoric etc.). Combined multivariate metabolome analysis, principal component hierarchical clustering, partial least square discriminant together identified 14 key mainly responsible distinguishing reversible vs. irreversible These are indicators lesions, structural/functional perturbations components (cell wall, chloroplast, vacuole, plasma membrane, nucleic acids), disruption metabolic homeostasis (antioxidant system, cytoplasmic pH, Kreb's cycle), protein degradation, hypoxia.

Язык: Английский

Процитировано

0

Data-Driven Characterization of Metabolome Reprogramming during Early Development of Sorghum Seedlings DOI Creative Commons
Ian A. Dubery, Lerato Nephali, Fidele Tugizimana

и другие.

Metabolites, Год журнала: 2024, Номер 14(2), С. 112 - 112

Опубликована: Фев. 7, 2024

Specialized metabolites are produced via discrete metabolic pathways. These small molecules play significant roles in plant growth and development, as well defense against environmental stresses. include damping off or seedling blight at a post-emergence stage. Targeted metabolomics was followed to gain insights into metabolome changes characteristic of different developmental stages sorghum seedlings. Metabolites were extracted from leaves seven time points post-germination analyzed using ultra-high performance liquid chromatography coupled mass spectrometry. Multivariate statistical analysis combined with chemometric tools, such principal component analysis, hierarchical clustering orthogonal partial least squares–discriminant applied for data exploration reduce dimensionality the selection potential discriminant biomarkers. Changes patterns seedlings early, middle, late (7, 14, 29 days post-germination). The metabolite classes amino acids, organic lipids, cyanogenic glycosides, hormones, hydroxycinnamic acid derivatives, flavonoids, latter representing largest class metabolites. In general, content showed an increase progression stages. Most differential derived tryptophan phenylalanine, which contribute innate immune defenses growth. Quantitative identified correlation apigenin flavone derivatives Data-driven investigations these metabolomes provided new dynamics that occur limit mortality.

Язык: Английский

Процитировано

3

Physiological and biochemical response of strawberry cv. diamond to nano zeolite soil application and cinnamic acid foliar application DOI Creative Commons
Najmeh Zeinalipour, Safoora Saadati

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 21, 2024

Abstract The increasing global population and climate change are pressing challenges that necessitate innovative agricultural solutions to ensure food security. One promising approach is the use of nano fertilizers foliar spraying techniques, which enhance nutrient delivery plants. purpose this research was examine how three different concentrations zeolite (0, 30, 60 mg L − 1 ) applied soil through irrigation cinnamic acid 100, 200 µM) topically affected physiological biochemical characteristics strawberry ( Fragaria × ananassa Duch.) cv. diamond, as well crop’s yield quality its fruits. A factorial system with replications used in experiment, designed a completely randomized design. According data, all features except stomatal conductivity were considerably impacted by application acid. Moreover, (200 spray combined (60 decreased electrolyte leakage, transpiration rate, malondialdehyde, while enhanced chlorophyll content, net photosynthesis leaf dry matter, relative water fruit length diameter, yield, parameters.

Язык: Английский

Процитировано

3

Time-course transcriptomic analyses integrated with metabolic data of Malus ‘Kelsey’ leaves in response to Gymnosporangium yamadae infection DOI
Yonghua Zhang, Qing Xu,

L.-L. Li

и другие.

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102649 - 102649

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Hydroxytyrosol as a Mitochondrial Homeostasis Regulator: Implications in Metabolic Syndrome and Related Diseases DOI Creative Commons
Jie Xu, Wei He, Zhenyu Sun

и другие.

Antioxidants, Год журнала: 2025, Номер 14(4), С. 398 - 398

Опубликована: Март 27, 2025

Hydroxytyrosol (HT), a principal bioactive phytochemical abundant in Mediterranean dietary sources, has emerged as molecule of significant scientific interest owing to its multifaceted health-promoting properties. Accumulating evidence suggests that HT’s therapeutic potential metabolic disorders extends beyond conventional antioxidant capacity encompass mitochondrial regulatory networks. This review synthesizes contemporary from our systematic investigations and the existing literature delineate comprehensive modulatory effects on homeostasis. We systematically summarized impact HT dynamics (fusion/fission equilibrium), biogenesis energy metabolism, mitophagy, inter-organellar communication with endoplasmic reticulum, microbiota–mitochondria crosstalk. Through this multidimensional analysis, we established homeostasis modulator applications syndrome (MetS) related pathologies including type 2 diabetes mellitus, obesity-related dysfunction, dyslipidemia, non-alcoholic steatohepatitis, hypertension-related complications. Moreover, further discussed translational challenges research, emphasizing imperative for direct target identification, mitochondrial-targeted delivery system development, combinatorial strategies. Collectively, provides mechanistic framework advancing research accelerating clinical implementation MetS diseases.

Язык: Английский

Процитировано

0

Structural Variations of Broccoli Polyphenolics and Their Antioxidant Capacity as a Function of Growing Temperature DOI Creative Commons
Ivana Šola,

Daria Gmižić

Plants, Год журнала: 2025, Номер 14(8), С. 1186 - 1186

Опубликована: Апрель 11, 2025

Polyphenolics in plants exist free, soluble-bound, and insoluble-bound structural forms. The concentration of these forms depends on the plant’s developmental stage, tissue type, soil water availability, food preparation methods. In this study, for first time, effects growth temperature (RT—room temperature—23 °C day/18 night, HT—high temperature—38 day/33 LT—low temperature—12 day/7 night) variations polyphenolic forms—free, soluble-bound (esterified glycosylated), insoluble-bound—in broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) microgreens were investigated. Using spectrophotometric, RP-HPLC, statistical analyses, it was found that highest amount total phenolics (TP) present esterified form, regardless at which they grown (63.21 ± 3.49 mg GAE/g dw RT, 65.55 8.33 HT, 77.44 7.82 LT). LT significantly increased free (from 13.30 2.22 RT to 18.33 3.85 dw) soluble TP 63.21 dw), while HT glycosylated 14.85 1.45 17.84 1.20 dw). also enhanced flavonoids, tannins, hydroxycinnamic acids, flavonols. other hand, TP, flavonols, phenolic decreased tannins. According ABTS method, induced antioxidant potential forms, capacity only. FRAP Also, based assays, polyphenolics showed higher than any form. Principal component analysis form had a greater impact temperature. Hierarchical clustering RT-, HT- LT-broccoli most similar their polyphenolics, but differed distinct overall. conclusion, specific shifts capacity. Based results, we recommend applying increase microgreens, may be used enhance

Язык: Английский

Процитировано

0

Endophytic mycobiont provides growth benefits via a phenylpropanoid-auxin axis in host plants DOI Creative Commons
Cheng‐Yen Chen, Naweed I. Naqvi

Cell Reports, Год журнала: 2025, Номер 44(5), С. 115648 - 115648

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Combined treatment of heat shock and glycine betaine recovered chilling injury in banana fruit by reducing membrane damage DOI Creative Commons
Nadia Niaz, Wanfeng Hu, Siyi Pan

и другие.

Food Chemistry X, Год журнала: 2025, Номер unknown, С. 102529 - 102529

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0