Abnormal Histopathological Expression of Klotho, Ferroptosis, and Circadian Clock Regulators in Pancreatic Ductal Adenocarcinoma: Prognostic Implications and Correlation Analyses DOI Creative Commons
Cielo García‐Montero, Oscar Fraile‐Martínez,

David Cobo-Prieto

и другие.

Biomolecules, Год журнала: 2024, Номер 14(8), С. 947 - 947

Опубликована: Авг. 5, 2024

Pancreatic ductal adenocarcinoma (PDAC) is an extremely lethal tumor with increasing incidence, presenting numerous clinical challenges. The histopathological examination of novel, unexplored biomarkers offers a promising avenue for research, significant translational potential improving patient outcomes. In this study, we evaluated the prognostic significance ferroptosis markers (TFRC, ALOX-5, ACSL-4, and GPX-4), circadian clock regulators (CLOCK, BMAL1, PER1, PER2), KLOTHO in retrospective cohort 41 patients deceased by PDAC. Immunohistochemical techniques (IHC) multiple statistical analyses (Kaplan-Meier curves, correlograms, multinomial linear regression models) were performed. Our findings reveal that are directly associated PDAC mortality, while inversely associated. Notably, TFRC emerged as strongest risk marker mortality (HR = 35.905), whereas CLOCK was identified most protective 0.01832). Correlation indicate positively correlated each other, regulators, which also correlate expression. contrast, exhibit inverse correlations markers. Among variables examined, only presence chronic pathologies showed association expression patterns several proteins studied. These underscore complexity pathogenesis highlight need further research into specific molecular mechanisms driving disease progression.

Язык: Английский

Dysregulation of Circadian Markers, HAT1 and Associated Epigenetic Proteins, and the Anti-Aging Protein KLOTHO in Placenta of Pregnant Women with Chronic Venous Disease DOI Open Access
Oscar Fraile‐Martínez, Cielo García‐Montero,

Tatiana Pekarek

и другие.

Journal of Personalized Medicine, Год журнала: 2025, Номер 15(3), С. 107 - 107

Опубликована: Март 9, 2025

Background: Chronic venous disease (CVD) is a vascular disorder common among pregnant women, due to the impairment in function associated with mechanical, hemodynamical, and hormonal changes that occur during pregnancy. CVD linked hypertension, inflammation, oxidative stress, hypoxia, which alter placental structure function, as demonstrated previous works. The placenta fulfills several roles fetal development maternal well-being by mediating nutrient exchange; acting chemical, immunological shield; producing essential hormones, making it crucial investigate effects of this organ. Patients methods: This work specifically analyzes gene expression circadian markers (CLOCK, BMAL1, PER1, PER2), epigenetic regulators (HAT1 molecules like histones H3, H4, RBBP7, ASF1), anti-aging protein KLOTHO tissue women (CVD-PW, N = 98) compared healthy controls (HC-PW, 82), using RT-qPCR immunohistochemistry (IHC) determine expression. Results: Our study demonstrates placentas CVD-PW exhibit reduced levels (clock, bmal1, per1, per2), increased hat1 related proteins (h3, h4, rbbp7, asf1), decreased klotho expression, indicative accelerated aging. Conclusions: These findings highlight profound molecular disturbances CVD, offering insights into disease’s pathophysiology potential implications for maternofetal well-being. While deepens our understanding relationship between dysfunction, further research required fully elucidate these mechanisms their long-term effects.

Язык: Английский

Процитировано

0
Abnormal Histopathological Expression of Klotho, Ferroptosis, and Circadian Clock Regulators in Pancreatic Ductal Adenocarcinoma: Prognostic Implications and Correlation Analyses DOI Creative Commons
Cielo García‐Montero, Oscar Fraile‐Martínez,

David Cobo-Prieto

и другие.

Biomolecules, Год журнала: 2024, Номер 14(8), С. 947 - 947

Опубликована: Авг. 5, 2024

Pancreatic ductal adenocarcinoma (PDAC) is an extremely lethal tumor with increasing incidence, presenting numerous clinical challenges. The histopathological examination of novel, unexplored biomarkers offers a promising avenue for research, significant translational potential improving patient outcomes. In this study, we evaluated the prognostic significance ferroptosis markers (TFRC, ALOX-5, ACSL-4, and GPX-4), circadian clock regulators (CLOCK, BMAL1, PER1, PER2), KLOTHO in retrospective cohort 41 patients deceased by PDAC. Immunohistochemical techniques (IHC) multiple statistical analyses (Kaplan-Meier curves, correlograms, multinomial linear regression models) were performed. Our findings reveal that are directly associated PDAC mortality, while inversely associated. Notably, TFRC emerged as strongest risk marker mortality (HR = 35.905), whereas CLOCK was identified most protective 0.01832). Correlation indicate positively correlated each other, regulators, which also correlate expression. contrast, exhibit inverse correlations markers. Among variables examined, only presence chronic pathologies showed association expression patterns several proteins studied. These underscore complexity pathogenesis highlight need further research into specific molecular mechanisms driving disease progression.

Язык: Английский

Процитировано

2