Biomolecules, Год журнала: 2024, Номер 14(9), С. 1178 - 1178
Опубликована: Сен. 19, 2024
Pancreatic cancer remains one of the most lethal malignancies due to its aggressive nature and resistance conventional therapies. This study investigates anti-proliferative, pro-apoptotic, anti-migratory effects Gemcitabine (GC) Withaferin A (WFA) on pancreatic cell lines PANC-1 Hs766t. The MTS assay revealed that both compounds effectively inhibit proliferation, with WFA showing a stronger effect in Hs766t cells. Flow cytometry analysis demonstrated GC WFA, particularly combination, significantly induce apoptosis lines. Migration assays confirmed potent inhibition migration by compounds, combination treatment being effective. Furthermore, actin cytoskeleton indicated substantial changes morphology stiffness, suggesting disrupt structural integrity Additionally, highlights ROS-mediated mechanism underlying as evidenced increased ROS levels following treatment, which were attenuated N-acetylcysteine. Importantly, NF-κB activity was modulated, reducing activation induced GC, potentially contributing synergistic pro-apoptotic combination. These findings suggest may offer therapeutic approach for treating targeting multiple aspects tumor behavior.
Язык: Английский