Crystals, Год журнала: 2025, Номер 15(2), С. 185 - 185
Опубликована: Фев. 15, 2025
Serial crystallography (SX) enables the determination of biologically relevant structures at room temperature while minimizing radiation damage. During SX experiments, beam center on diffraction images can shift due to X-ray movements or detector displacement. Consequently, geometry file for is optimized; however, effects deviations from optimal position data processing efficiency remain unclear. This study examines how changes in influence quality by analyzing indexing and structure refinement lysozyme glucose isomerase datasets, considering shifts parameter. The results revealed that as deviated farther its position, declined, with extent effect varying significantly across algorithms. XDS MOSFLM algorithms maintained high efficiencies (>90%) ≤4 pixels (688 μm) ≤2 (344 μm), respectively, compared processed optimized center. Conversely, DirAx XGANDALF exhibited below 90% a two-pixel These findings enhance our understanding affect provide valuable insights developing effective strategies.
Язык: Английский