Frit-Inlet Asymmetric Flow Field-Flow Fractionation for the Analysis of Lipid Nanoparticle-Protein Interactions DOI Creative Commons

Rand Abdulrahman,

Panida Punnabhum,

Robin Capomaccio

и другие.

Journal of Chromatography A, Год журнала: 2025, Номер 1743, С. 465663 - 465663

Опубликована: Янв. 11, 2025

Research into nanoparticle interactions with biomolecules has become increasingly important in nanomedicine. While lipid nanoparticles (LNPs) are widely used as drug delivery systems, there remains a gap understanding their fate circulation, which is crucial for selecting appropriate lipids during formulation development. This study the first to use Asymmetric Flow Field Fractionation (AF4) compare two types of LNPs: MC3-LNPs and SM-102-LNPs, model protein, bovine serum albumin (BSA). AF4 offers high-resolution separation, ability simultaneously perform multiparametric inline analysis multiple detectors. In this study, impact LNP size, morphology PDI on BSA corona formation were examined using multiangle light scattering (MALS) dynamic (DLS). separation revealed subpopulations MC3-LNPs, while SM102-LNPs exhibited single population. Analysis shape factor indicated 0.783 SM-102-BSA 0.741 0.795 (peak 1 2) MC3-BSA, confirming interaction between LNPs BSA. Both LNP-BSA induced aggregation. Overall, demonstrates effectiveness AF4, particularly when hyphenated multidetector separating from complex biological media studying LNP-protein interactions.

Язык: Английский

Frit-Inlet Asymmetric Flow Field-Flow Fractionation for the Analysis of Lipid Nanoparticle-Protein Interactions DOI Creative Commons

Rand Abdulrahman,

Panida Punnabhum,

Robin Capomaccio

и другие.

Journal of Chromatography A, Год журнала: 2025, Номер 1743, С. 465663 - 465663

Опубликована: Янв. 11, 2025

Research into nanoparticle interactions with biomolecules has become increasingly important in nanomedicine. While lipid nanoparticles (LNPs) are widely used as drug delivery systems, there remains a gap understanding their fate circulation, which is crucial for selecting appropriate lipids during formulation development. This study the first to use Asymmetric Flow Field Fractionation (AF4) compare two types of LNPs: MC3-LNPs and SM-102-LNPs, model protein, bovine serum albumin (BSA). AF4 offers high-resolution separation, ability simultaneously perform multiparametric inline analysis multiple detectors. In this study, impact LNP size, morphology PDI on BSA corona formation were examined using multiangle light scattering (MALS) dynamic (DLS). separation revealed subpopulations MC3-LNPs, while SM102-LNPs exhibited single population. Analysis shape factor indicated 0.783 SM-102-BSA 0.741 0.795 (peak 1 2) MC3-BSA, confirming interaction between LNPs BSA. Both LNP-BSA induced aggregation. Overall, demonstrates effectiveness AF4, particularly when hyphenated multidetector separating from complex biological media studying LNP-protein interactions.

Язык: Английский

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