Fundamentals of laboratory diagnostics in allergology
Allergo Journal International,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 26, 2025
Язык: Английский
Grundlagen der Labordiagnostik in der Allergologie
Allergo Journal,
Год журнала:
2025,
Номер
34(2), С. 16 - 29
Опубликована: Март 1, 2025
Molecular Signatures of Aeroallergen Sensitization in Pediatric Populations: A Comparative Study Across Spanish Cities
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 2963 - 2963
Опубликована: Март 25, 2025
Spain
exhibits
significant
climatic
variability
across
its
regions,
ranging
from
the
humid
oceanic
climate
in
north
to
Mediterranean
and
stepped
climates
southern
central
areas.
These
differences
influence
environmental
allergen
exposure,
which
may,
turn,
affect
allergic
sensitization
patterns
pediatric
population.
Variations
temperature,
humidity,
airborne
distribution,
such
as
pollen,
dust
mites,
animal
epithelia,
mold,
contribute
regional
disparities
responses
among
children.
Understanding
how
conditions
shape
recognition
different
geographical
areas
is
essential
for
improving
allergy
prevention
management
strategies.
This
study
aims
shed
light
on
this
issue
by
identifying
a
population
of
236
patients
(with
2
age
groups
analyzed:
6-10
11-15
years
old)
regions
Spain.
Skin
tests
with
standard
aeroallergens
were
performed,
specific
IgE
(sIgE)
analysis
Allergy
Explorer
Macroarray
Diagnostic
test
(ALEX2)
Western
blot.
The
results
revealed
trends
eight
cities
analyzed
(Barcelona,
Granada,
Lugo,
Sagunto,
Santiago
de
Compostela,
Valencia,
Zaragoza).
Язык: Английский
Validation of a Multiplex Molecular Macroarray for the Determination of Allergen-Specific IgE Sensitizations in Dogs
Veterinary Sciences,
Год журнала:
2024,
Номер
11(10), С. 482 - 482
Опубликована: Окт. 7, 2024
Detecting
IgE
sensitizations
in
the
serum
of
allergic
dogs
is
commonly
performed
using
allergen
extracts,
but
these
are
difficult
to
standardize.
This
article
details
development
and
validation
Pet
Allergy
Xplorer
(PAX;
Nextmune,
Stockholm,
Sweden),
first
multiplex
macroarray
for
detection
sensitization
extracts
molecular
components;
PAX
derived
from
(ALEX
Язык: Английский
Protein Biochips with Three-Dimensional Hydrogel or Polymer Brush Elements for the Detection of Human Serum Immunoglobulin E Specific to Inhalation Allergens
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 13047 - 13047
Опубликована: Дек. 4, 2024
The
development
of
rapid
analysis
human
serum
for
the
presence
allergen-specific
Immunoglobulin
E
(IgE)
is
currently
important.
Consequently,
we
developed
two
types
three-dimensional
(3D)
protein
biochips.
first
one
a
3D
hydrogel
biochip
containing
droplets
with
molecules
(allergens,
immunoglobulins
and
others).
These
are
disposed
on
elements
consisting
short
polymer
brushes
grafting
from
surface
polybutylene
terephthalate
polymer.
immobilization
proteins
was
induced
by
short-wave
ultraviolet
(UV)
radiation.
On
such
biochip,
kinetics
allergen-sIgE
complex
formation
reached
60%
saturation
6
h.
Also,
brush
microchip
polyethylene
covalently
immobilized
opening
oxirane
cycles
amino
thiol
nucleophilic
groups
contained
in
proteins.
In
case
microchip,
100%
3
h,
fluorescent
signals
were
2-3
times
higher
than
those
some
allergens.
Thus,
comparative
revealed
that
biochips
more
useful
further
studies
protein-protein
interaction
ones.
Язык: Английский
Molecular Signatures of Aeroallergen Sensitization in Respiratory Allergy: A Comparative Study Across Climate-Matched Populations
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 284 - 284
Опубликована: Дек. 31, 2024
Climate
change
is
significantly
altering
the
dynamics
of
airborne
allergens,
affecting
their
seasonality,
allergenicity,
and
geographic
distribution,
which
correlates
with
increasing
rates
allergic
diseases.
This
study
investigates
aeroallergen
sensitization
among
populations
from
Tenerife,
Spain,
Lima,
Peru—two
regions
similar
climates
but
distinct
socio-economic
conditions.
Our
findings
reveal
that
Spanish
individuals,
particularly
those
asthma,
demonstrate
higher
levels
to
a
broader
range
especially
mites,
85%
participants
reacting
at
least
one
mite
allergen.
In
contrast,
Peruvian
patients
exhibit
narrower
spectrum
sensitization.
These
results
highlight
influence
environmental
factors,
such
as
pollution
disparities,
on
allergen
exposure
immune
responses.
Moreover,
this
underscores
necessity
for
region-specific
diagnostic
therapeutic
strategies
effectively
address
these
variations.
By
elucidating
intricate
relationship
between
climate
change,
sensitization,
research
offers
insights
into
respiratory
conditions,
advocating
tailored
interventions
mitigate
impact
across
diverse
populations.
Язык: Английский