Neuroprotective Effects of Hesperidin and CK2 Inhibitor DRB on Aβ1-42-Induced Neurotoxicity in Differentiated SH-SY5Y Cells DOI Creative Commons
Hamiyet Eci̇roglu, Pınar Altın Çelik, Pelin Kelicen‐Uğur

и другие.

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

Abstract There is still no approved treatment for Alzheimer’s disease (AD), a progressive neurodegenerative disorder characterized by amyloid plaques, neurofibrillary tangles, and synaptic dysfunction. In an in vitro AD model, this study aimed to comparatively assess the neuroprotective effects of citrus flavonoid Hesperidin casein kinase 2 (CK2) inhibitor 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole (DRB) as potential therapeutic targets AD. First, SH-SY5Y neuroblastoma cells were differentiated into cholinergic neuron-like using all -trans retinoic acid (RA) brain-derived neurotrophic factor (BDNF). Then, generate 20 μM Aβ 1–42 was applied induce neurotoxicity cells. The CK2 DRB on model evaluated MTT, RT-qPCR, ELISA methods. Both DRB, at high concentrations, reduced cell viability 24 48 h ( p < 0.05 0.01). Pre-treatment with 25 50 µM 0.25 0.5 increased ADAM10 gene expression decreased BACE1 expression, both which are associated markers, compared 1-42 group 0.05). concentrations CK2α 0.05), whereas had effect > pre-treatment significantly levels 0.01), p-Tau (T181) Bax/Bcl-2 ratio As result, our showed that inhibited production suppressing amyloidogenic pathway activating non-amyloidogenic while also exerting inhibitory neuronal apoptosis. may be target could contribute pathophysiology However, these findings should validated further studies.

Язык: Английский

Neuroprotective Effects of Hesperidin and CK2 Inhibitor DRB on Aβ1-42-Induced Neurotoxicity in Differentiated SH-SY5Y Cells DOI Creative Commons
Hamiyet Eci̇roglu, Pınar Altın Çelik, Pelin Kelicen‐Uğur

и другие.

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

Abstract There is still no approved treatment for Alzheimer’s disease (AD), a progressive neurodegenerative disorder characterized by amyloid plaques, neurofibrillary tangles, and synaptic dysfunction. In an in vitro AD model, this study aimed to comparatively assess the neuroprotective effects of citrus flavonoid Hesperidin casein kinase 2 (CK2) inhibitor 5,6-dichloro-1-β-D-ribofuranosyl benzimidazole (DRB) as potential therapeutic targets AD. First, SH-SY5Y neuroblastoma cells were differentiated into cholinergic neuron-like using all -trans retinoic acid (RA) brain-derived neurotrophic factor (BDNF). Then, generate 20 μM Aβ 1–42 was applied induce neurotoxicity cells. The CK2 DRB on model evaluated MTT, RT-qPCR, ELISA methods. Both DRB, at high concentrations, reduced cell viability 24 48 h ( p < 0.05 0.01). Pre-treatment with 25 50 µM 0.25 0.5 increased ADAM10 gene expression decreased BACE1 expression, both which are associated markers, compared 1-42 group 0.05). concentrations CK2α 0.05), whereas had effect > pre-treatment significantly levels 0.01), p-Tau (T181) Bax/Bcl-2 ratio As result, our showed that inhibited production suppressing amyloidogenic pathway activating non-amyloidogenic while also exerting inhibitory neuronal apoptosis. may be target could contribute pathophysiology However, these findings should validated further studies.

Язык: Английский

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