Gut microbiome community profiling of Bornean bats with different feeding guilds
Animal Microbiome,
Год журнала:
2025,
Номер
7(1)
Опубликована: Март 5, 2025
Bats
are
extraordinary
mammals.
They
have
evolved
to
consume
various
dietary
sources,
such
as
insects,
fruits,
nectar,
blood,
and
meat.
This
diversity
has
generated
considerable
interest
in
the
scientific
community,
resulting
efforts
leverage
bats
model
organisms
study
correlation
between
diet
gut
microbiome
community.
Although
studies
now
commonly
use
Next
Generation
Sequencing
(NGS),
similar
early
their
development
Southeast
Asia,
especially
Malaysia,
which
harbours
an
incredibly
diverse
bat
fauna.
provides
pioneering
NGS
metabarcoding
information
on
Bornean
bats.
By
using
a
high-throughput
Nanopore-based
16S
rRNA
gene
sequencing
method,
Bacillota,
Pseudomonadota,
Campylobacterota
were
found
insectivorous
phytophagous
Both
groups
harboured
no
dominant
taxon
(D
=
0.076;
D
0.085).
A
comparative
analysis
of
bacteria
functional
identified
eight
major
both
bats,
with
fermentation
being
predominant
group.
The
network
revealed
negative
'good
bacteria'
Lactobacillus
pathogenic
genera,
Salmonella
(-0.4124)
Yersinia
(-0.4654),
demonstrating
its
prebiotic
characteristics.
broadens
our
understanding
from
diets,
emphasis
new
data
Borneo.
Язык: Английский
Herbal formula alleviates heat stress by improving physiological and biochemical attributes and modulating the rumen microbiome in dairy cows
Frontiers in Veterinary Science,
Год журнала:
2025,
Номер
12
Опубликована: Март 7, 2025
Heat
stress
significantly
impacts
dairy
cow
productivity,
health,
and
welfare.
This
study
evaluated
a
self-developed
herbal
formula
as
dietary
intervention
to
mitigate
heat
stress.
A
total
of
198
lactating
cows
were
divided
into
two
groups:
Control
group
receiving
standard
mixed
rations
Herbs
supplemented
with
for
60
days.
Various
parameters
assessed,
including
milk
yield
composition,
antioxidant
capacity,
immune
responses,
stress-related
gene
expression,
rumen
microbial
composition.
Compared
the
group,
in
showed
improved
feed
intake,
quality,
rumination
frequency,
enhanced
activity
response.
Rumen
microbiome
analysis
revealed
reduced
relative
abundance
Proteobacteria
Ochrobactrum
along
an
enrichment
beneficial
genera
such
Lachnospira
.
Functional
predictions
indicated
that
exhibited
glycolysis/gluconeogenesis,
pyruvate
metabolism,
starch
sucrose
reflecting
fermentation
efficiency
energy
utilization.
In
conclusion,
physiological
biochemical
attributes,
boosted
modulated
microbiome,
contributing
alleviation
cows.
These
findings
highlight
its
potential
natural
strategy
support
health
productivity
under
conditions.
Язык: Английский
Unlocking the Potential of Metagenomics with the PacBio High-Fidelity Sequencing Technology
Microorganisms,
Год журнала:
2024,
Номер
12(12), С. 2482 - 2482
Опубликована: Дек. 2, 2024
Traditional
methods
for
studying
microbial
communities
have
been
limited
due
to
difficulties
in
culturing
and
sequencing
all
species.
Recent
advances
third-generation
technologies,
particularly
PacBio’s
high-fidelity
(HiFi)
sequencing,
significantly
advanced
metagenomics
by
providing
accurate
long-read
sequences.
This
review
explores
the
role
of
HiFi
overcoming
limitations
previous
methods,
including
high
error
rates
fragmented
assemblies.
We
discuss
benefits
applications
across
various
environments,
such
as
human
gut
soil,
which
provides
broader
context
further
exploration.
Key
studies
are
discussed
highlight
sequencing’s
ability
recover
complete
coherent
genomes
from
complex
microbiomes,
showcasing
its
superior
accuracy
continuity
compared
other
technologies.
Additionally,
we
explore
potential
quantitative
analysis,
well
detection
single
nucleotide
variations
(SNVs)
structural
(SVs).
PacBio
is
establishing
a
new
benchmark
metagenomics,
with
enhance
our
understanding
ecology
drive
forward
advancements
both
environmental
clinical
applications.
Язык: Английский
Characteristics and Differences in the Antler Velvet Microbiota During Regeneration
Microorganisms,
Год журнала:
2024,
Номер
13(1), С. 36 - 36
Опубликована: Дек. 27, 2024
The
skin
surface
has
a
complex
and
dynamic
ecosystem
inhabited
by
diverse
microbiota.
wound
formed
antler
velvet
shedding
can
naturally
achieve
regenerative
restoration,
but
the
changes
in
microbial
composition
that
occur
during
regeneration
are
largely
unknown.
In
this
study,
we
analyzed
microbiota
of
sika
deer
at
15
days
(Half)
30
(Full)
post-pedicle
casting
using
16S
rRNA
gene
sequencing.
A
total
2659
OTUs
were
identified,
which
assigned
to
26
phyla,
304
families,
684
genera.
core
two
groups
mainly
composed
Atopostipes
spp.,
Corynebacterium
Burkholderia
Staphylococcus
Paracoccus
spp.
comparison
with
Full
group,
Shannon,
Simpson,
Ace,
Chao
1
indices
significantly
decreased
Half
group
(p
<
0.05).
Principal
coordinate
analysis
showed
there
significant
differences
community
between
based
on
Bray-Curtis
dissimilarity,
weighted
Unifrac
distance,
unweighted
distance
relative
abundances
bacteria
belonging
genera
Staphylococcus,
Romboutsia_B,
Dietzia
increased
while
Atopostipes,
Psychrobacter,
Faecousia
Correlation
positively
correlated
arginine
proline
metabolism
These
findings
demonstrate
is
accompanied
distinct
highlight
potential
roles
key
taxonomy
healing
tissue
regeneration.
Язык: Английский