Recent advances in polysaccharide-dominated extracellular polymeric substances from microalgae: A review

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140572 - 140572

Опубликована: Фев. 1, 2025

Язык: Английский

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Exopolysaccharides from the Green Microalga Strain Coelastrella sp. BGV—Isolation, Characterization, and Assessment of Anticancer Potential

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(9), С. 10312 - 10334

Опубликована: Сен. 16, 2024

Algal metabolites have been extensively studied as potential anticancer therapeutics. Among them, polysaccharides attracted much attention because of their beneficial biological effects and safety. In the present research, chemical characteristics, antitumor, proapoptotic activities extracellular (EPS) isolated from a new Bulgarian strain green microalga Coelastrella sp. BGV were investigated. A fast convenient method precipitation with cold ethanol was used to isolate EPS culture medium. The characteristics examined by colorimetric spectrophotometric analyses, HPSEC-RID HPLC-UV chromatography, FT-IR spectroscopy. results showed that sample consists three carbohydrate fractions different molecular weights (11.5 × 104 Da, 30.7 72.4 respectively) contains 7.14 (w/w%) protein. analysis revealed presence galactose fucose. total uronic acid content in 4.5 (w/w%). IR-FT spectrum various functional groups typical polysaccharide (or proteoglycan) composed primarily neutral sugars. obtained assessed using cell lines cancerous non-cancerous origins vitro experimental models. performed MTT assay reduced viability cervical mammary carcinoma HeLa MCF-7, while control non-cancer BALB/3T3 HaCaT less affected. line highest sensitivity therefore for further studies its potential. ability inhibit cancer migration demonstrated wound-healing (scratch) assay. cycle FACS indicated treatment induced significant increases sub G1 population decreases percentages cells G1, S, G2-M phases, compared control. fluorescent microscopy staining methods combination Annexin V-FITC flow cytometric clearly demonstrate induce death via apoptosis pathway. Moreover, an altered pattern intensity immunocytochemical apoptosis- proliferation-related proteins p53, bcl2, Ki67 detected EPS-treated untreated controls. characterize local microalgae producer selective antitumor activity provide opportunity pharmacological biotechnological

Язык: Английский

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Exploring the Effects of Seaweed Synthesized Nanoparticles on Human Cancer Cell Lines

Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Seaweeds can be found in either marine or brackish water and have the potential to bioactive. are important ecological, biological, regenerative components of maritime environment. Because their high concentration polysaccharides phytochemicals, they sparked a lot interest domains cosmetics, biomass for fuels, as source material nanoparticle synthesis. Phytochemicals seaweeds aid conversion metal ions nanoparticles, which easily overcome tumor micro-environment barrier cause damage cancer cells. In addition, nanotechnology-based medicine has promising therapeutic effect treatment. Functionalized nanoparticles improve effects anticancer drugs while also delivering them site over time. Metal attention scientific community use biomedical nanotechnology. Drug-loaded were more hazardous cells than bare drugs.

Язык: Английский

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Physicochemical characterization and evaluation of the antioxidant potential of water-soluble polysaccharides from red microalgae, Rhodomonas salina

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143417 - 143417

Опубликована: Апрель 1, 2025

Язык: Английский

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Comparative Evaluation of Dynamic Maceration and Ultrasonic Assisted Extraction of Fucoidan from Four Arctic Brown Algae on Its Antioxidant and Anticancer Properties

Marine Drugs, Год журнала: 2025, Номер 23(6), С. 230 - 230

Опубликована: Май 28, 2025

The technology of fucoidan extraction significantly affects its properties. This study aimed to evaluate the impact dynamic maceration (DM) and ultrasound-assisted (UAE) on antioxidant anticancer properties from Arctic brown algae. Fucus vesiculosus (Fv), serratus (Fs), distichus (Fd), Ascophyllum nodosum (An) were collected Barents Sea. average yield uronic acid was higher (by 43.2% 22.0%, respectively) after UAE, while phlorotannin content decreased by 53.7% compared with DM. fucose level for all algae increased molecular weight fucoidans lower. highest activity noted An Fv, which obtained DM can be associated high concentrations phlorotannins. treatment HeLa G-63 cells studied 48 h concentration-dependently number dead cells. most promising Fv Fs phlorotannins, low sulfates, extracted co-administration paclitaxel caused cell cycle arrest in G2/M phase. calculated first time combinatory effect showed that simultaneous use exposure leads a synergistic interaction. Our results support rationality complex chemotherapy improve survival, quality life immunity patients cervical carcinoma.

Язык: Английский

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Exploring the versatility of Porphyridium sp.: A comprehensive review of cultivation, bio-product extraction, purification, and characterization techniques

Biotechnology Advances, Год журнала: 2024, Номер 77, С. 108471 - 108471

Опубликована: Окт. 20, 2024

Язык: Английский

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Exploring Porphyridium purpureum and Porphyridium aerugineum as alternative resources for phycobiliprotein production

Bioresource Technology, Год журнала: 2024, Номер 416, С. 131800 - 131800

Опубликована: Ноя. 12, 2024

Язык: Английский

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