Construction of an Efficient Engineered Strain for Chaetoglobosin A Bioresource Production from Potato Starch Industrial Waste

Foods, Год журнала: 2025, Номер 14(5), С. 842 - 842

Опубликована: Фев. 28, 2025

Chaetoglobosin A (CheA), a typical structure of the cytochalasin family, exhibits outstanding efficacy against variety tumor cells and plant pathogens. However, its low yield high production cost are major obstacles limiting wide application. In order to increase CheA yield, an engineered strain was established by overexpressing CgMfs, gene encoding MFS family's efflux pump, on chassis lacking CgXpp1, which have been shown act as negative regulator biosynthesis. As expected, significantly boosted from 63.19 265.93 mg/L after incubation in PDA medium for 10 d, whereas remarkably enhanced 2.93-fold compared with wild type, following d cultivation utilizing potato starch industrial waste. The addition metal ions had positive effect production, Cu2+ being most effective improving 176.92 mg/L. optimal fermentation conditions were determined response surface optimization, under conditions, could stably produce 197.58 This study provided reducing costs while increasing well new strategies insights target compound.

Язык: Английский

Optimization of Process Parameters for Single Cell Protein Production from <i>Trichoderma </i><i>atroviride</i> Strain Using Cassava Peel Substrates

Food and Nutrition Sciences, Год журнала: 2025, Номер 16(05), С. 517 - 535

Опубликована: Янв. 1, 2025

Язык: Английский