Fluorescent Assay for the Determination of Enrofloxacin Using an Aptamer and SYBR Green I DOI

Wei‐Chuang Kong,

Chenchen Li,

Xiaolei Zhang

и другие.

Analytical Letters, Год журнала: 2024, Номер unknown, С. 1 - 13

Опубликована: Ноя. 26, 2024

Enrofloxacin (ENR) is commonly used to treat bacterial infections in animals and can pose a potential threat human health with overuse. Therefore, the development of an accurate method for enrofloxacin urgently needed. In this work, we have developed based on aptamer SYBR Green I (SG-I). To our knowledge, strategy first time enrofloxacin. The had G-quadruplex structure, which be identified modified by SG-I emit strong fluorescence. presence analyte, preferentially bound causing conformational change double-stranded portion aptamer, caused fluorescence quenching. exhibited consistent reduction proportion concentrations from 1.95 400 nM. limit detection was 0.59 Furthermore, sensor employed determine food serum recovery rates ranging 98.04% 103.08%.

Язык: Английский

A colorimetric-aptamer-based assay for the determination of enrofloxacin through triggering the aggregation of gold nanoparticles DOI

Wei‐Chuang Kong,

Chenchen Li,

Ai-Hong Zhang

и другие.

Analytical Methods, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Although enrofloxacin (ENR) is a widely used broad-spectrum antibiotic in veterinary medicine, its residues animals can pose risk to human health. Thus, we developed new method for detecting ENR based on aptamers and AuNPs. In the absence of ENR, attached surface AuNPs

Язык: Английский

Процитировано

5
Enhanced Detection of Enrofloxacin in Seawater Using a Newly Selected Aptamer on a Graphite Oxide-Based Biosensor DOI
Sin Yu Lam, Pan Li,

Linjie Jin

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Developing aptasensors offers several advantages including sensitivity, selectivity, cost-effectiveness, and speed over traditional analytical techniques for antibiotic detection. We have successfully identified Enro_ap3, a 30-mer enrofloxacin-binding aptamer with micromolar binding affinity, through an optimized Capture-SELEX platform. Compared to other reported aptamers, this shorter not only streamlines the design process but also eliminates common issue of strong nonspecific GO surface, thereby improving overall detection capabilities biosensor (GO-Enro_ap3-FAM). This aptasensor demonstrated remarkable selectivity by effectively distinguishing enrofloxacin from different structurally diverse antibiotics. The sensor boasts LOD 32.15 μg/mL, 2.5 times more sensitive than original 30-mer, recoveries 74%-92% relative standard deviations 6.3%-12.5% in seawater samples spiked enrofloxacin. Furthermore, aptasensor's were found be on par LC-MS/MS techniques, exhibiting no significant differences recovery rates even complex matrices. sensor's performance remained consistent across variations salinity, acidity, total organic carbon concentrations collected locations, underlining its robustness environmental conditions suitability real-world monitoring applications. Our findings highlight importance aptamer's chain length affinity toward target after immobilization substrate. These factors significantly impact seawater. Overall, provides well-balanced approach, combining adaptability, practical usability detecting pharmaceutical contaminants, such as antibiotics, marine environments.

Язык: Английский

Процитировано

0
A label-free aptasensor based on a fluorescent probe containing styrylbenzothiazole for detection of enrofloxacin DOI

Wei‐Chuang Kong,

Chenchen Li,

Juntao Niu

и другие.

Analytical Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

We have successfully constructed a fluorescent aptasensor based on an ENR aptamer and the probe PBTZM containing styrylbenzothiazole that enabled rapid, label-free detection of with high sensitivity.

Язык: Английский

Процитировано

0
Proton Conductive 2D MXene-derived Potassium Titanate Nanoribbons Fabricated Electrochemical Platform for Trace Detection of Enrofloxacin DOI
Arghya Chakravorty, Vimala Raghavan

Chemosphere, Год журнала: 2024, Номер 366, С. 143520 - 143520

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1
Recent Advancements in Biosensors Using Biopolymers DOI
Mariam M. Abady,

Amal Mohamed Shawky,

Fathi A. Sakr

и другие.

ACS symposium series, Год журнала: 2024, Номер unknown, С. 81 - 112

Опубликована: Ноя. 1, 2024

Biopolymers, derived from renewable sources, offer a promising avenue for advancing sensor technology due to their unique properties and eco-friendly nature. This chapter explores recent advancements in leveraging biopolymers, highlighting biocompatibility, sustainability, diverse functionality. The integration of biopolymers sensors addresses the growing demand sensitive, selective, environmentally friendly sensing solutions across various domains. Through physicochemical characterization development biopolymer-based technologies, researchers have unlocked innovative applications healthcare, environmental monitoring, beyond. However, challenges such as stability, reliability, production cost, scalability persist, hindering widespread adoption sensors. Addressing these requires continued research efforts optimize performance commercial viability. Despite obstacles, transformative potential underscores significance shaping future applications. As we navigate towards this future, it is imperative overcome existing hurdles fully harness capabilities drive innovation development.

Язык: Английский

Процитировано

1
Graphene oxide-based fluorescence analytical methods for bioassays DOI

S. V. Ahir,

Vimalkumar Prajapati, Swati Patel

и другие.

Comprehensive analytical chemistry, Год журнала: 2024, Номер unknown, С. 117 - 138

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Fluorescence detection for enrofloxacin by using Aptamer and fluorophore SYBR Green I DOI

Chenchen Li,

Wei‐Chuang Kong,

Jia-Le Cui

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 11, 2024

Abstract Enrofloxacin (ENR), as a type of antimicrobial, is commonly used in animal sterilization. However, excessive ENR residues food will seriously harm human health. Therefore, this work, we developed quantitative fluorescence method for based on aptamer and SYBR Green I (SG-I). DNA aptamers with G-quadruplex space structure can be recognized inserted by SG-I to emit intense green fluorescence. In the presence ENR, preferentially bound specifically resulting change conformation, which prevented from inserting into cause quenching. The sensor quantitatively detect linear concentration range 0-600 nM, limit detection was 0.158 nM. detecting chicken, fish pork samples recovery rate 99.74–104.02%, relative standard deviation (RSD) less than 6%. aptamer-based provided safety.

Язык: Английский

Процитировано

0
Fluorescent Assay for the Determination of Enrofloxacin Using an Aptamer and SYBR Green I DOI

Wei‐Chuang Kong,

Chenchen Li,

Xiaolei Zhang

и другие.

Analytical Letters, Год журнала: 2024, Номер unknown, С. 1 - 13

Опубликована: Ноя. 26, 2024

Enrofloxacin (ENR) is commonly used to treat bacterial infections in animals and can pose a potential threat human health with overuse. Therefore, the development of an accurate method for enrofloxacin urgently needed. In this work, we have developed based on aptamer SYBR Green I (SG-I). To our knowledge, strategy first time enrofloxacin. The had G-quadruplex structure, which be identified modified by SG-I emit strong fluorescence. presence analyte, preferentially bound causing conformational change double-stranded portion aptamer, caused fluorescence quenching. exhibited consistent reduction proportion concentrations from 1.95 400 nM. limit detection was 0.59 Furthermore, sensor employed determine food serum recovery rates ranging 98.04% 103.08%.

Язык: Английский

Процитировано

0