Journal of life sciences and agriculture.,
Год журнала:
2024,
Номер
1(4), С. 47 - 54
Опубликована: Дек. 1, 2024
This
experiment
aims
to
explore
the
diverse
beneficial
effects
of
Lactobacillus
plantarum.
Employing
dilution
coating
method,
eighteen
strains
were
isolated
and
screened
from
commercially
available
pickled
cucumbers.
Subsequent
evaluations
carried
out
assess
their
probiotic
potential,
including
examinations
gastrointestinal
tolerance
antioxidant
activity.
Through
these
analyses,
a
highly
adaptable
strain
was
identified.
The
results
demonstrate
that
designated
as
DC
-
3
exhibits
remarkable
cholesterol
lowering
capabilities.
Moreover,
it
sustains
survival
rate
exceeding
70%
in
bovine
bile
salt
media
with
concentrations
ranging
0.3%
1%,
even
within
environment,
where
level
can
reach
up
75%.
cell's
capacity
scavenge
DPPH
radicals
hydroxyl
is
37.73%
46.71%,
respectively.
Additionally,
self
aggregation
co
abilities
are
64%
28.5%,
These
findings
suggest
presents
potential
provides
valuable
reference
information
for
future
development
L.
plantarum
related
fermented
food.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 8, 2025
Abstract
Antimicrobial
resistance
(AMR)
is
a
global
health
challenge
requiring
cross-sector
action,
with
research
largely
focused
on
chickens,
leaving
ducks
underexplored.
This
study
examines
AMR
dynamics
in
Ross
308
broilers
and
Cherry
Valley
over
15
months
stocking
periods
under
consistent
rearing
conditions.
A
total
of
96
pooled
samples
were
collected:
50
from
broiler
farms
(26
biological,
24
environmental)
46
duck
(24
22
environmental).
Using
next-generation
shotgun
sequencing,
3,665
distinct
types
identified:
1,918
1,747
ducks.
Host-specific
AMRs
comprised
25.3%
18%
ducks,
while
56.7%
shared.
diversity
declined
across
production
phases,
losing
641
308,
yet
frequencies
increased
significantly
by
the
finisher
phase
(
p
<
0.0001).
Based
silico
data,
prophylactic
antibiotic
use
reduced
prevalence
multidrug-resistant
bacteria
both
poultry
species
0.05).
Hospital-acquired
infection-associated
higher
than
at
start
but
end
period
Above-average
markers
accounted
for
approximately
10%
all
detected
determinants.
Tetracycline
phenicol
resistances
emerged
as
most
prevalent.
13
high-resistance
carrier
(HRC)
shared
between
hosts.
Broiler-specific
HRCs
exhibited
abundances
(relative
frequency:
0.08)
duck-specific
0.003,
=
0.035).
The
grower
critical
intervention
point.
In
farm
environments
broiler-specific
9
biomarker
identified,
each
strongly
correlated
poultry-core
(correlation
coefficient
>
0.7).
Broiler
key
genes,
tetracycline
predominantly
associated
Bacteroides
coprosuis
,
Pasteurella
multocida
Acinetobacter
baumannii
.
Despite
its
limitations,
this
provides
insights
into
trends
two
major
types,
guiding
targeted
interventions
sustainable
management
strategies.
Microorganisms,
Год журнала:
2025,
Номер
13(1), С. 160 - 160
Опубликована: Янв. 14, 2025
Lactobacillus
is
a
key
genus
of
probiotics
commonly
utilized
for
the
treatment
oral
infections
The
primary
aim
our
research
was
to
investigate
probiotic
potential
newly
isolated
Levilactobacillus
brevis
DPL5
strain
from
human
breast
milk,
focusing
on
its
ability
combat
biofilm-forming
pathogens
such
as
Staphylococcus
aureus.
Employing
in
vitro
approaches,
we
demonstrate
L.
DPL5′s
endure
at
pH
3
with
survival
rates
above
30%,
and
withstand
osmotic
stress
often
found
during
industrial
processes
like
fermentation
freeze
drying,
retaining
over
90%
viability.
lyophilized
cell-free
supernatant
had
significant
antagonistic
effect
against
biofilm-producing
nasal
strains
aureus,
it
completely
eradicated
biofilms
subinhibitory
concentrations
20
mg·mL−1.
Higher
69
mg·mL−1
were
have
99%
bactericidal
effect,
based
conducted
probability
analysis,
indicating
production
bioactive
extracellular
compounds
capable
disrupting
biofilm
formation
S.
Furthermore,
genome-wide
sequencing
analysis
cutting-edge
Nanopore
technology
has
uncovered
50
genes
linked
activity,
supporting
adapt
thrive
harsh
gut
environment.
genome
also
contains
multiple
biosynthetic
gene
clusters
lanthipeptide
class
IV,
Type
III
polyketide
synthase
(T3PKS),
ribosomally
synthesized,
post-translationally
modified
peptides
(RiPP-like
compounds),
all
which
are
associated
antibacterial
properties.
Our
study
paves
way
further
exploration
DPL5,
setting
stage
innovative,
nature-inspired
solutions
stubborn
bacterial
infections.
Fermentation,
Год журнала:
2025,
Номер
11(4), С. 217 - 217
Опубликована: Апрель 15, 2025
This
paper
comprehensively
reviews
whey,
a
by-product
of
cheese
production,
as
raw
material
for
various
biotechnological
applications.
It
addresses
its
unique
composition,
the
environmental
impact
inadequate
disposal,
and
opportunities
it
offers
to
develop
high-value
products
in
line
with
circular
economy
sustainability
principles.
Using
PRISMA
methodology,
systematic
search
was
conducted
databases
(Science
Direct,
Scopus,
Google
Scholar)
specific
inclusion
exclusion
criteria.
Studies
from
last
five
years
were
considered,
focusing
on
food
applications,
production
bioproducts
(such
lactic
acid,
biopolymers,
bioethanol,
biomass,
enzymes),
use
whey
culture
medium
expression
recombinant
proteins.
is
concluded
that
applications
mitigates
associated
disposal
represents
an
economic
sustainable
alternative
industrial
bioproducts.
The
integration
pretreatment
technologies,
experimental
designs,
improvements
producing
strains
brings
these
processes
closer
competitive
conditions
industry,
opening
new
perspectives
innovation
fermentation
sector.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 29, 2025
Abstract
In
the
last
few
years,
probiotics
have
gained
much
attention
within
medical,
pharmaceutical,
and
food
fields,
given
health
benefits
provided
by
their
consumption.
They
include
several
lactic
acid
bacteria
(LAB)
species,
mostly
belonging
to
genera
Lactobacillus,
Lactococcus,
Streptococcus.
Postbiotics
are
bioactive
compounds
(organic
acids,
short-chain
fatty
enzymes,
neurotransmitters)
produced
bacterial
fermentation
that
exert
different
effects.
It
is
well
known
probiotics,
as
health-promoting
microorganisms,
show
therapeutic
properties,
including
anti-pathogenic,
anti-inflammatory,
cholesterol-lowering
activities.
Recently,
accumulating
evidence
has
shown
certain
commensal
play
protective
roles
against
cancer;
thus,
anti-carcinogenic
activity
one
of
most
interesting
properties
currently
under
investigation.
Here,
we
studied
anticancer
postbiotics
three
Lactococcus
lactis
subsp
lactis
strains
isolated
from
natural
whey
starter
cultures
on
human
glioblastoma
cell
lines.
MTT
Trypan
Blue
exclusion
assays
revealed
a
significant
reduction
in
proliferation,
flow
cytometry
analysis
corroborated
this
data,
demonstrating
cycle
arrest
treated
cells.
Moreover,
other
cancer
hallmarks,
such
wound
healing
rate
closure
migration,
were
markedly
inhibited
postbiotics.
On
hand,
primary
astrocytes
viability
blood-brain
barrier
(BBB)
integrity
not
impaired,
suggesting
selective
effect
proliferating-undifferentiated
This
preliminary
study
highlights,
for
first
time,
potential
some
L.
