Luteolin Mitigates Photoaging Caused by UVA-Induced Fibroblast Senescence by Modulating Oxidative Stress Pathways

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1809 - 1809

Опубликована: Фев. 20, 2025

As a polyphenolic plant flavone, luteolin (Lut) is widely found in many medicinal plants, flowers, and vegetables. Although Lut has been shown to have the effect of preventing treating skin photoaging, its role photoaging specifically induced by ultraviolet A (UVA) radiation remains underreported. In vivo, BALB/c mice were used as models for treated with Lut. Additionally, NIH-3T3 fibroblasts utilized vitro further investigate whether exerts anti-photoaging effects enhancing fibroblast vitality function. Several biochemical assays (CCK-8, catalase, superoxide dismutase, malondialdehyde, dichloro-dihydro-fluorescein diacetate, quantitative real-time-PCR, gene expression patterns) histochemical (histological staining, immunofluorescent SA-β-Gal experiments, western blotting analysis) conducted. The findings demonstrate that pretreatment could enhance function both vivo experiments inhibit UVA-induced collagen degradation, thereby improving skin's resistance photoaging. We confirmed inhibited senescent factors P21, P16, pro-inflammatory senescence-associated secretory phenotype (SASP) factors. exhibited potent antioxidant during UVA exposure. Bioinformatics network pharmacology analyses revealed Lut's may be mediated through regulation oxidative stress-related pathways anti-aging genes. Upon utilizing inhibitors agonists stress, we prevents senescence suppressing ultimately protects from damage. These indicate lutein mitigates caused modulation stress pathways.

Язык: Английский

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Polyphenol Microneedles for Dermatological Therapy

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract Dermatological diseases represent one of the most prevalent health concerns globally, with associated care and treatment costs having consistently increased over past decade. Current therapeutic modalities, including topical medications, oral pharmaceuticals, physical therapies, frequently face challenges such as inadequate drug absorption substantial adverse effects. Microneedles, recognized minimally invasive delivery systems, effectively penetrate cutaneous barrier to enhance permeability efficacy. Polyphenols—abundant in botanical marine sources—demonstrate diverse pharmacological properties encompassing anti‐inflammatory antioxidant activities. The integration polyphenols microneedle technology has emerged a promising strategy for dermatological conditions, demonstrating significant clinical potential. This review examines multifunctional capabilities polyphenol‐incorporated microneedles elucidates their mechanisms action managing disorders. Furthermore, it synthesizes recent progress polyphenol‐based applications various pathologies, acne vulgaris, psoriasis, atopic dermatitis. Finally, current is discussed proposed future research directions optimizing polyphenol systems therapeutics.

Язык: Английский

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Anthraquinones and Aloe Vera Extracts as Potential Modulators of Inflammaging Mechanisms: A Translational Approach from Autoimmune to Onco-Hematological Diseases

Molecules, Год журнала: 2025, Номер 30(6), С. 1251 - 1251

Опубликована: Март 11, 2025

Inflammaging is a chronic, low-grade inflammatory state that contributes to age-related diseases, including cardiovascular disorders, osteoporosis, neurodegeneration, and cancer. This process involves immunosenescence, oxidative stress, immune aging, all of which contribute the breakdown tolerance onset autoimmune disorders. Aloe vera (AV) has recently gained attention for its immunomodulatory, anti-inflammatory, antioxidant properties. review explores effects AV extracts anthraquinones (e.g., aloe–emodin, emodin, aloin) on key inflammaging-driven mechanisms in autoimmunity. Our analysis highlights AV’s ability regulate hormone balance, autoantibody production, cytokine/chemokine signaling (such as interleukin-1β, tumor necrosis factor-α, interferon-γ). It modulates pathways, mitogen-activated protein kinases (MAPKs) phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT), thereby inhibiting nuclear factor kappa-light-chain-enhancer activated B-cell (NF-κB) activation. Additionally, enhances defenses restores balance by reducing Th1/Th17 subsets while promoting Th2-mediated regulation. Notably, also inflammasome-mediated counteracts driven autophagy-related processes. These position potential integrative approach mitigating Furthermore, inflammaging increasingly recognized onco-hematological AV-based strategies may offer novel therapeutic avenues. Future studies should focus clinical validation, optimizing formulations, expanding applications broader immune-mediated

Язык: Английский

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Green biosynthesis of silver nanoparticles using anthocyanins-rich extract from Euterpe edulis fruits (AnthocyanOx®): assessment in vitro of antioxidant and antiglycation activities, and in silico anti-aging activity

Food and Bioproducts Processing, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Phytochemical profiling of Pelargonium graveolens l’Hér and evaluation of its activity against D-galactose induced skin ageing

Natural Product Research, Год журнала: 2025, Номер unknown, С. 1 - 13

Опубликована: Апрель 5, 2025

Pelargonium graveolens L'Hér is an evergreen small shrub with pink flowers. Metabolite profiling of its extract by UHPLC/MS analysis revealed the identification 51 secondary metabolites. Interestingly, 29 compounds identified for first time. The colorimetric assays high content phenolic, flavonoids and tannins (100.19 ± 3.94 µg Gallic acid Eq/mg, 47.38 2.04 Rutin Eq/mg 332.75 8.85 Catechin extract) respectively along good antioxidant activity. Moreover, in vitro showed a significant anti-hyaluronidase activity (IC50 SD = 15.75 0.8), as compared to standard luteolin. Topical application characterised P. hydrogel (1% 2% w/w) was tested using D-galactose-induced ageing model mice. Histopathological examination demonstrated increase dermal collagen expression, alleviation ageing-related oxidative stress, stabilisation MMP-1/TIMP-1 balance. Ultimately, could be valuable source natural antioxidants promising antiaging

Язык: Английский

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Phytochemical Investigation and Biological Activities of Ruta chalepensis Methanolic Extract: Antioxidant, Anti‐Inflammatory, Anticollagenase, and Antielastase Properties

Journal of Food Biochemistry, Год журнала: 2024, Номер 2024(1)

Опубликована: Янв. 1, 2024

Ruta chalepensis has long been recognized in traditional medicine for its diverse pharmacological properties. The primary aim of this study was to analyze the phytochemical composition and evaluate antioxidant, anti‐inflammatory, anticollagenase, antielastase activities methanolic extract derived from R. . subjected screening using LC‐MS analysis. For evaluation antioxidant activity, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) assays were used. anti‐inflammatory properties investigated a protein denaturation bioassay. Finally, fluorometric kits assessed anticollagenase activities. identified 33 compounds, dominant being 3,3′,4,5,7‐pentahydroxyflavone (9.4%), myricetin (8.9%). showed with IC 50 values 30.1 μ g/mL DPPH assay 25.4 ABTS assay. Moreover, displayed significant inhibition across range concentrations. Additionally, exhibited dose‐dependent inhibitory effect on collagenase elastase highest concentration tested, 5%, achieved 41.7% 62.4% inhibition. In summary, showcases promising enzyme activities, hinting at potential wide array therapeutic applications.

Язык: Английский

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Anti-Graying Effects of External and Internal Treatments with Luteolin on Hair in Model Mice

Antioxidants, Год журнала: 2024, Номер 13(12), С. 1549 - 1549

Опубликована: Дек. 17, 2024

Little is known about the anti-graying effects of antioxidants on hair. The three (luteolin, hesperetin, and diosmetin) hair were investigated according to sequential processes graying that previously clarified in model mice [Ednrb(+/−);RET-mice]. External treatment with luteolin, but not hesperetin or diosmetin, alleviated Ednrb(+/−);RET-mice. Internal luteolin also mitigated mice. Although both treatments had very limited cycles, suppressed increase p16ink4a-positive cells bulges [senescent keratinocyte stem (KSCs)]. Both decreases expression levels endothelins KSCs their receptor (Ednrb) melanocyte (MSCs) Luteolin a special antioxidant an potency through improvement age-related dysfunction signaling between MSCs. for topical oral use commercially available people form supplements. Similar Ednrb(+/−);RET-mice humans have been reported. These results are encouraging practical application as medicine effect humans.

Язык: Английский

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Repurposing the plant-derived compound apigenin for senomorphic effect in antiaging pipelines

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Cellular senescence is a cell fate triggered by inherent or environmental stress and characterized stable cycle arrest accompanied hypersecretory feature, termed as the senescence-associated secretory phenotype (SASP). Senescent burden increases with natural aging, functionally contributing to age-related organ dysfunction multiple disorders. In this study, we performed large scale screening of product library for senotherapeutic candidates assessing their effects on human senescent cells. Apigenin, dietary flavonoid previously reported antioxidant anti-inflammatory activities, exhibited prominent capacity in targeting cells senomorphic agent. cells, apigenin blocks interactions between ATM/p38 HSPA8, thus preventing transition acute stress-associated (ASAP) towards SASP. Mechanistically, targets peroxiredoxin 6 (PRDX6), an intracellular redox-active molecule, suppressing iPLA2 activity PRDX6 disrupting downstream reactions underlying SASP development. Without reversing cellular senescence, deprives cancer malignancy acquired from stromal culture, while reducing chemoresistance upon combination chemotherapy anticancer regimens. preclinical trials, administration improves physical function animals prematurely aged after whole body irradiation, alleviating frailty cognitive impairment. Overall, our study demonstrates potential exploiting naturally derived compound achieve geroprotective modulating SASP, providing research platform future exploration novel agents against conditions.

Язык: Английский

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Evaluation of cosmetic efficacy of lychee seed fermentation liquid

Bioresources and Bioprocessing, Год журнала: 2024, Номер 11(1)

Опубликована: Окт. 31, 2024

Lychee seeds were fermented by three kinds of bacteria (Lactobacillus plantarum, Saccharomyces cerevillus and ganoderma lucidum mycelium), two effective strains selected indexes activity content antioxidant, so as to further verify whether lychee have waste multiplication effect can protect cells damaged oxidation from anti-inflammatory, anti-aging safety.

Язык: Английский

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Antiaging Properties of Kalanchoe blossfeldiana Ethanol Extract—Ex Vivo and In Vitro Studies

Molecules, Год журнала: 2024, Номер 29(23), С. 5548 - 5548

Опубликована: Ноя. 24, 2024

Species of the genus

Язык: Английский

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