Lyophilized and Oven-Dried Manilkara zapota Extracts: Characterization and In Vitro, In Vivo, and In Silico Analyses DOI Creative Commons

María Fernanda Rivas-Gastélum,

Patricia Ariadna Galindo-Castillo,

Juan Esparza-Sánchez

и другие.

Plants, Год журнала: 2025, Номер 14(2), С. 216 - 216

Опубликована: Янв. 14, 2025

In this work, extracts from the pulp, peel, and seed of Manilkara zapota were obtained via lyophilization oven drying. Bromatological analyses performed to investigate variabilities in nutritional content fruits after nine post-harvest days. The phytochemical was assessed by gas chromatography flame ionization detector (GC-FID), their biological performance studied using vitro antibacterial antioxidant assays (DPPH ABTS) vivo toxicity models. Molecular docking implemented evaluate interaction between polar compounds chicozapote with receptors involved pathogenesis bacterial strains. Results revealed that water or soluble solids did not vary post-harvest. It demonstrated oven-drying approaches influenced insoluble, total dietary fiber digestible carbohydrates among samples. According GC-FID analysis, it observed methods also altered myristic pentadecanoic acids extracts. noted activities weak due MIC (>1000 μg/mL) IC50 (>2000 values. Still, poor against Artemia salina nauplii. silico evaluation unveiled M. possess a high binding affinity towards DNA gyrase B cultured This study expands scientific evidence regarding influence distinct extraction on nutraceutical native importance considering additional enhance bioactivities.

Язык: Английский

Azithromycin-Loaded Nanoparticles Incorporated in Chitosan-Based Soft Hydrogels: A Novel Approach for Dental Drug Delivery DOI Creative Commons

Jakub Kwiatek,

Magdalena Paczkowska-Walendowska, Anna Rył

и другие.

Pharmaceutics, Год журнала: 2025, Номер 17(3), С. 304 - 304

Опубликована: Фев. 26, 2025

Background: Azithromycin (AZC), a BCS class II/IV antibiotic with broad-spectrum antimicrobial activity, has poor water solubility, limiting its formulation potential. This study aimed to develop and optimize AZC-based soft hydrogels for the first time improved local controlled drug release, dental applications. Methods: AZC nanoparticles (based on polyvinylpyrrolidone) were synthesized via electrospinning enhanced solubility 40-fold. These incorporated into chitosan (CS) varying concentrations degrees of deacetylation (DDA), optimized using factorial design. Hydrogels characterized mucoadhesion, antioxidant, anti-inflammatory, properties, Principal Component Analysis (PCA) assessing correlations. Results: Soft 3% CS 80% DDA achieved sustained release (62.9–94.7% over 48 h), strong biological activity. Higher antioxidant anti-inflammatory effects due increased free amino groups. Antimicrobial tests showed efficacy against Streptococcus mutans Staphylococcus aureus. PCA revealed an inverse correlation between mucoadhesion positive correlations Conclusions: significantly showing potential delivery. Further clinical validation optimization are recommended.

Язык: Английский

Процитировано

1

Lyophilized and Oven-Dried Manilkara zapota Extracts: Characterization and In Vitro, In Vivo, and In Silico Analyses DOI Creative Commons

María Fernanda Rivas-Gastélum,

Patricia Ariadna Galindo-Castillo,

Juan Esparza-Sánchez

и другие.

Plants, Год журнала: 2025, Номер 14(2), С. 216 - 216

Опубликована: Янв. 14, 2025

In this work, extracts from the pulp, peel, and seed of Manilkara zapota were obtained via lyophilization oven drying. Bromatological analyses performed to investigate variabilities in nutritional content fruits after nine post-harvest days. The phytochemical was assessed by gas chromatography flame ionization detector (GC-FID), their biological performance studied using vitro antibacterial antioxidant assays (DPPH ABTS) vivo toxicity models. Molecular docking implemented evaluate interaction between polar compounds chicozapote with receptors involved pathogenesis bacterial strains. Results revealed that water or soluble solids did not vary post-harvest. It demonstrated oven-drying approaches influenced insoluble, total dietary fiber digestible carbohydrates among samples. According GC-FID analysis, it observed methods also altered myristic pentadecanoic acids extracts. noted activities weak due MIC (>1000 μg/mL) IC50 (>2000 values. Still, poor against Artemia salina nauplii. silico evaluation unveiled M. possess a high binding affinity towards DNA gyrase B cultured This study expands scientific evidence regarding influence distinct extraction on nutraceutical native importance considering additional enhance bioactivities.

Язык: Английский

Процитировано

0