Abstract
Crosslinking
amine‐rich
biomolecules
with
aldehydes,
such
as
paraformaldehyde
(PFA)
and
glutaraldehyde
(GA),
is
a
widely
used
method
for
biomaterial
fabrication
cell
fixation.
In
this
study,
we
introduce
homobifunctional
photocaged
2‐butene‐1,4‐dials
(bis‐PBDAs)
novel
amine‐reactive
photo‐crosslinkers.
Compared
to
PFA
GA,
bis‐PBDAs
are
inherently
less
toxic.
Upon
photo‐deprotection,
efficiently
react
the
amino
groups
of
biomolecules,
carboxymethyl
chitosan
histone
H3,
forming
stable,
well‐defined
crosslinks.
Even
at
low
concentrations
(~100
μM),
facilitate
effective
fixation
without
significantly
altering
cellular
morphology
or
interfering
biochemical
analyses,
including
protein/mRNA
extraction
immunofluorescence
imaging.
Therefore,
promising
reagents
crosslinking
biopolymers
hold
great
potential
in
living
cells
tissues.
Polymers,
Год журнала:
2024,
Номер
16(3), С. 344 - 344
Опубликована: Янв. 26, 2024
Chitosan
is
a
linear
polyelectrolyte
with
active
hydroxyl
and
amino
groups
that
can
be
made
into
chitosan-based
hydrogels
by
different
cross-linking
methods.
Chitosan-based
also
have
three-dimensional
network
of
hydrogels,
which
accommodate
large
number
aqueous
solvents
biofluids.
CS,
as
an
ideal
drug-carrying
material,
effectively
encapsulate
protect
drugs
has
the
advantages
being
nontoxic,
biocompatible,
biodegradable.
These
make
it
material
for
preparation
functional
act
wound
dressings
skin
injuries.
This
review
reports
role
in
promoting
repair
context
mechanisms
involved
injury
repair.
were
found
to
promote
at
process
stages.
Various
are
discussed.
Biosensors,
which
combine
physical
transducers
with
biorecognition
elements,
have
seen
significant
advancement
due
to
the
heightened
interest
in
rapid
diagnostic
technologies
across
a
number
of
fields,
including
medical
diagnostics,
environmental
monitoring,
and
food
safety.
In
particular,
polydiacetylene
(PDA)
is
gaining
attention
as
an
ideal
material
for
label-free
colorimetric
biosensor
development
its
unique
color-changing
properties
response
external
stimuli.
PDA
forms
through
self-assembly
diacetylene
monomers,
color
change
occurring
conjugated
backbone
twists
stimuli
such
temperature,
pH,
chemical
interactions.
This
enables
detection
biomarkers,
metal
ions,
toxic
compounds.
Moreover,
combination
polymeric
structures
hydrogels
further
enhances
sensitivity
structural
stability
PDA-based
biosensors,
making
them
reliable
effective
complex
biological
conditions.
review
comprehensively
examines
recent
research
trends
applications
PDA–polymeric
structure
hybrid
while
discussing
future
directions
potential
advancements
this
field.
Chitosan-based
hydrogels
have
gained
significant
attention
for
their
potential
applications
in
water
treatment
and
purification
due
to
remarkable
properties
such
as
bioavailability,
biocompatibility,
biodegradability,
environmental
friendliness,
high
pollutants
adsorption
capacity,
capacity.
This
article
comprehensively
reviews
recent
advances
chitosan-based
hydrogel
materials
applications.
The
synthesis
methods,
structural
properties,
performance
of
are
critically
analyzed.
incorporation
various
nanomaterials
into
hydrogels,
nanoparticles,
graphene,
metal-organic
frameworks,
has
been
explored
enhance
performance.
mechanisms
purification,
including
adsorption,
filtration,
antimicrobial
activity,
also
discussed
detail.
the
removal
pollutants,
heavy
metals,
organic
contaminants,
microorganisms,
from
sources
is
highlighted.
Moreover,
challenges
future
perspectives
illustrated.
Overall,
this
provides
valuable
insights
current
state
art
regarding
highlights
addressing
global
pollution
challenges.
Although
titanium
and
alloys
have
become
the
preferred
materials
for
various
medical
implants,
surface
modification
technology
still
needs
to
be
strengthened
in
order
adapt
complex
physiological
environment
of
human
body.
Compared
with
physical
or
chemical
methods,
biochemical
modification,
such
as
introduction
functional
hydrogel
coating
on
can
fix
biomolecules
proteins,
peptides,
growth
factors,
polysaccharides,
nucleotides
so
that
they
directly
participate
biological
processes;
regulate
cell
adhesion,
proliferation,
migration,
differentiation;
improve
activity
implants.
This
review
begins
a
look
at
common
substrate
coatings
implant
surfaces,
including
natural
polymers
collagen,
gelatin,
chitosan,
alginate,
synthetic
polyvinyl
alcohol,
polyacrylamide,
polyethylene
glycol,
polyacrylic
acid.
Then,
construction
methods
(electrochemical
method,
sol–gel
method
layer-by-layer
self-assembly
method)
are
introduced.
Finally,
five
aspects
enhancement
effect
bioactivity
alloy
implants
described:
osseointegration,
angiogenesis,
macrophage
polarization,
antibacterial
effects,
drug
delivery.
In
this
paper,
we
also
summarize
latest
research
progress
point
out
future
direction.
After
searching,
no
previous
relevant
literature
reporting
information
was
found.
Gels,
Год журнала:
2024,
Номер
10(9), С. 547 - 547
Опубликована: Авг. 23, 2024
Regenerative
hydrogels
from
natural
polymers
have
come
forth
as
auspicious
materials
for
use
in
regenerative
medicine,
with
interest
attributed
to
their
intrinsic
biodegradability,
biocompatibility,
and
ability
reassemble
the
extracellular
matrix.
This
review
covers
latest
advances
used
wound
healing,
focusing
on
chemical
composition,
cross-linking
mechanisms,
functional
properties.
Key
carbohydrate
polymers,
including
alginate,
chitosan,
hyaluronic
acid,
polysaccharide
gums,
agarose,
carrageenan,
xanthan
gum,
are
discussed
terms
of
sources,
structures
specific
properties
suitable
applications.
The
further
explores
categorization
based
ionic
charge,
response
physiological
stimuli
(i.e.,
pH,
temperature)
particularized
roles
tissue
self-healing.
Various
methods
enhance
mechanical
biological
performance
these
also
examined.
By
highlighting
recent
innovations
ongoing
challenges,
this
article
intends
give
a
detailed
understanding
potential
revolutionize
medicine
improve
patient
healing
outcomes.
