Paternal preconception phthalate exposure alters sperm methylome and embryonic programming DOI Creative Commons
Oladele Oluwayiose, Chelsea Marcho, Haotian Wu

и другие.

Environment International, Год журнала: 2021, Номер 155, С. 106693 - 106693

Опубликована: Июнь 10, 2021

Preconception environmental conditions have been demonstrated to shape sperm epigenetics and subsequently offspring health development. Our previous findings in humans showed that urinary anti-androgenic phthalate metabolites males were associated with altered methylation blastocyst-stage embryo To corroborate this, we examined the effect of preconception exposure di(2-ethylhexyl) (DEHP) on genome-wide DNA gene expression profiles mice. Eight-week old C57BL/6J male mice exposed either a vehicle control, low, or high dose DEHP (2.5 25 mg/kg/weight, respectively) for 67 days (~2 spermatogenic cycles) mated unexposed females. Reduced representation bisulfite sequencing (RRBS) epididymal was performed gastrulation stage embryos collected RRBS transcriptome analyses both embryonic extra-embryonic lineages. Male resulted 704 differentially methylated regions (DMRs; q-value < 0.05; ≥10% change) sperm, 1,716 DMRs embryonic, 3,181 tissue. Of these, 29 overlapped between F1 tissues, half which concordant changes F0 generations. transcriptomes at E7.5 also by including developmental families such as Hox, Gata, Sox. Additionally, ontology expressed genes enrichment multiple processes development, pattern specification morphogenesis. These data indicate spermatogenesis adult may represent sensitive window alters methylome well developing embryo.

Язык: Английский

Dux cluster duplication ensures full activation of totipotent genes DOI Creative Commons
Meiqi Lin,

Zeling Du,

Dan Guo

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(10)

Опубликована: Март 6, 2025

Zygotic genome activation (ZGA) confers to the mouse two-cell (2C) embryo a unique transcriptional profile characterized by transient up-regulation of many totipotency-related genes and MERVL retrotransposons. Intriguingly, those are duplicated clustered in during evolution, including Dux cluster, Obox, Zscan4 family members mice. However, contribution biological significance gene duplication events early development remain poorly understood. Here, we focus on master regulator ZGA that is necessary sufficient for induction 2C-like cells embryonic stem (mESCs). By reducing copies from 31 0 or 1 through CRISPR-Cas9 technology, generate Dux-KO (n = 1) mESC lines, respectively. We uncover awakened much lesser extent mESCs compared wild type following global DNA demethylation reprogramming damage, mimicking intrinsic preimplantation development. Together, cluster critically required full transcripts.

Язык: Английский

Процитировано

1

The Nuclear-Mitochondrial Crosstalk in Aging: From Mechanisms to Therapeutics DOI
Yifei Feng, Yan Lu

Free Radical Biology and Medicine, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1

FTDC1/2, oocyte-specific cofactors of DNMT1 required for epigenetic regulation and embryonic development DOI

Congyang Li,

Jiashuo Li,

Siyu Du

и другие.

Cell Death and Differentiation, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Язык: Английский

Процитировано

1

Molecular Mechanisms of Facultative Heterochromatin Formation: An X-Chromosome Perspective DOI Open Access
Jan J. Żylicz, Édith Heard

Annual Review of Biochemistry, Год журнала: 2020, Номер 89(1), С. 255 - 282

Опубликована: Апрель 7, 2020

Facultative heterochromatin (fHC) concerns the developmentally regulated heterochromatinization of different regions genome and, in case mammalian X chromosome and imprinted loci, only one allele a homologous pair. The formation fHC participates timely repression genes, by resisting strong trans activators. In this review, we discuss molecular mechanisms underlying establishment maintenance mammals using mouse model. We focus on X-chromosome inactivation (XCI) as paradigm for but also relate it to genomic imprinting homeobox ( Hox) gene cluster repression. A vital role noncoding transcription and/or transcripts emerges general principle triggering XCI canonical imprinting. However, other types are established through an unknown mechanism, independent Hox clusters noncanonical imprinting). extensively polycomb-group repressive complexes (PRCs), which frequently play maintenance.

Язык: Английский

Процитировано

61

Paternal preconception phthalate exposure alters sperm methylome and embryonic programming DOI Creative Commons
Oladele Oluwayiose, Chelsea Marcho, Haotian Wu

и другие.

Environment International, Год журнала: 2021, Номер 155, С. 106693 - 106693

Опубликована: Июнь 10, 2021

Preconception environmental conditions have been demonstrated to shape sperm epigenetics and subsequently offspring health development. Our previous findings in humans showed that urinary anti-androgenic phthalate metabolites males were associated with altered methylation blastocyst-stage embryo To corroborate this, we examined the effect of preconception exposure di(2-ethylhexyl) (DEHP) on genome-wide DNA gene expression profiles mice. Eight-week old C57BL/6J male mice exposed either a vehicle control, low, or high dose DEHP (2.5 25 mg/kg/weight, respectively) for 67 days (~2 spermatogenic cycles) mated unexposed females. Reduced representation bisulfite sequencing (RRBS) epididymal was performed gastrulation stage embryos collected RRBS transcriptome analyses both embryonic extra-embryonic lineages. Male resulted 704 differentially methylated regions (DMRs; q-value < 0.05; ≥10% change) sperm, 1,716 DMRs embryonic, 3,181 tissue. Of these, 29 overlapped between F1 tissues, half which concordant changes F0 generations. transcriptomes at E7.5 also by including developmental families such as Hox, Gata, Sox. Additionally, ontology expressed genes enrichment multiple processes development, pattern specification morphogenesis. These data indicate spermatogenesis adult may represent sensitive window alters methylome well developing embryo.

Язык: Английский

Процитировано

45