Environment International,
Год журнала:
2021,
Номер
155, С. 106693 - 106693
Опубликована: Июнь 10, 2021
Preconception
environmental
conditions
have
been
demonstrated
to
shape
sperm
epigenetics
and
subsequently
offspring
health
development.
Our
previous
findings
in
humans
showed
that
urinary
anti-androgenic
phthalate
metabolites
males
were
associated
with
altered
methylation
blastocyst-stage
embryo
To
corroborate
this,
we
examined
the
effect
of
preconception
exposure
di(2-ethylhexyl)
(DEHP)
on
genome-wide
DNA
gene
expression
profiles
mice.
Eight-week
old
C57BL/6J
male
mice
exposed
either
a
vehicle
control,
low,
or
high
dose
DEHP
(2.5
25
mg/kg/weight,
respectively)
for
67
days
(~2
spermatogenic
cycles)
mated
unexposed
females.
Reduced
representation
bisulfite
sequencing
(RRBS)
epididymal
was
performed
gastrulation
stage
embryos
collected
RRBS
transcriptome
analyses
both
embryonic
extra-embryonic
lineages.
Male
resulted
704
differentially
methylated
regions
(DMRs;
q-value
<
0.05;
≥10%
change)
sperm,
1,716
DMRs
embryonic,
3,181
tissue.
Of
these,
29
overlapped
between
F1
tissues,
half
which
concordant
changes
F0
generations.
transcriptomes
at
E7.5
also
by
including
developmental
families
such
as
Hox,
Gata,
Sox.
Additionally,
ontology
expressed
genes
enrichment
multiple
processes
development,
pattern
specification
morphogenesis.
These
data
indicate
spermatogenesis
adult
may
represent
sensitive
window
alters
methylome
well
developing
embryo.
Proceedings of the National Academy of Sciences,
Год журнала:
2025,
Номер
122(10)
Опубликована: Март 6, 2025
Zygotic
genome
activation
(ZGA)
confers
to
the
mouse
two-cell
(2C)
embryo
a
unique
transcriptional
profile
characterized
by
transient
up-regulation
of
many
totipotency-related
genes
and
MERVL
retrotransposons.
Intriguingly,
those
are
duplicated
clustered
in
during
evolution,
including
Dux
cluster,
Obox,
Zscan4
family
members
mice.
However,
contribution
biological
significance
gene
duplication
events
early
development
remain
poorly
understood.
Here,
we
focus
on
master
regulator
ZGA
that
is
necessary
sufficient
for
induction
2C-like
cells
embryonic
stem
(mESCs).
By
reducing
copies
from
31
0
or
1
through
CRISPR-Cas9
technology,
generate
Dux-KO
(n
=
1)
mESC
lines,
respectively.
We
uncover
awakened
much
lesser
extent
mESCs
compared
wild
type
following
global
DNA
demethylation
reprogramming
damage,
mimicking
intrinsic
preimplantation
development.
Together,
cluster
critically
required
full
transcripts.
Annual Review of Biochemistry,
Год журнала:
2020,
Номер
89(1), С. 255 - 282
Опубликована: Апрель 7, 2020
Facultative
heterochromatin
(fHC)
concerns
the
developmentally
regulated
heterochromatinization
of
different
regions
genome
and,
in
case
mammalian
X
chromosome
and
imprinted
loci,
only
one
allele
a
homologous
pair.
The
formation
fHC
participates
timely
repression
genes,
by
resisting
strong
trans
activators.
In
this
review,
we
discuss
molecular
mechanisms
underlying
establishment
maintenance
mammals
using
mouse
model.
We
focus
on
X-chromosome
inactivation
(XCI)
as
paradigm
for
but
also
relate
it
to
genomic
imprinting
homeobox
(
Hox)
gene
cluster
repression.
A
vital
role
noncoding
transcription
and/or
transcripts
emerges
general
principle
triggering
XCI
canonical
imprinting.
However,
other
types
are
established
through
an
unknown
mechanism,
independent
Hox
clusters
noncanonical
imprinting).
extensively
polycomb-group
repressive
complexes
(PRCs),
which
frequently
play
maintenance.
Environment International,
Год журнала:
2021,
Номер
155, С. 106693 - 106693
Опубликована: Июнь 10, 2021
Preconception
environmental
conditions
have
been
demonstrated
to
shape
sperm
epigenetics
and
subsequently
offspring
health
development.
Our
previous
findings
in
humans
showed
that
urinary
anti-androgenic
phthalate
metabolites
males
were
associated
with
altered
methylation
blastocyst-stage
embryo
To
corroborate
this,
we
examined
the
effect
of
preconception
exposure
di(2-ethylhexyl)
(DEHP)
on
genome-wide
DNA
gene
expression
profiles
mice.
Eight-week
old
C57BL/6J
male
mice
exposed
either
a
vehicle
control,
low,
or
high
dose
DEHP
(2.5
25
mg/kg/weight,
respectively)
for
67
days
(~2
spermatogenic
cycles)
mated
unexposed
females.
Reduced
representation
bisulfite
sequencing
(RRBS)
epididymal
was
performed
gastrulation
stage
embryos
collected
RRBS
transcriptome
analyses
both
embryonic
extra-embryonic
lineages.
Male
resulted
704
differentially
methylated
regions
(DMRs;
q-value
<
0.05;
≥10%
change)
sperm,
1,716
DMRs
embryonic,
3,181
tissue.
Of
these,
29
overlapped
between
F1
tissues,
half
which
concordant
changes
F0
generations.
transcriptomes
at
E7.5
also
by
including
developmental
families
such
as
Hox,
Gata,
Sox.
Additionally,
ontology
expressed
genes
enrichment
multiple
processes
development,
pattern
specification
morphogenesis.
These
data
indicate
spermatogenesis
adult
may
represent
sensitive
window
alters
methylome
well
developing
embryo.
