Transgenic sugarcane overexpressing Glyoxalase III improved germination and biomass production at formative stage under salinity and water-deficit stress conditions

3 Biotech, Год журнала: 2024, Номер 14(2)

Опубликована: Янв. 23, 2024

Язык: Английский

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Genome-Wide Identification and Drought Stress Response Pattern of the NF-Y Gene Family in Cymbidium sinense

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(5), С. 3031 - 3031

Опубликована: Март 6, 2024

Cymbidium sinense, a type of orchid plant, is more drought-resistant and ornamental than other terrestrial orchids. Research has shown that many members the NUCLEAR FACTOR Y (NF-Y) transcription factor family are responsive to plant growth, development, abiotic stress. However, mechanism NF-Y gene family’s response stress in orchids not yet been reported. In this study, phylogenetic analysis allowed for 27 CsNF-Y genes be identified (5 CsNF-YAs, 9 CsNF-YBs, 13 CsNF-YC subunits), CsNF-Ys were homologous those Arabidopsis Oryza. Protein structure revealed different subfamilies contained motifs, but all them Motif 2. Secondary tertiary protein indicated CsNF-YB had high content alpha helix structures. Cis-element showed elements related drought mainly concentrated subfamilies, with CsNF-YB3 CsNF-YC12 having highest content. The results transcriptome there was trend downregulation almost leaves under stress, while roots, most subfamily upregulation. Additionally, seven selected real-time reverse quantitative PCR (qRT-PCR) experiments. generally consistent analysis. regulatory roles 1, 2, 4 particularly evident roots. findings our study may make great contribution understanding role stress-related metabolic processes.

Язык: Английский

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Transgenic sugarcane with higher levels of BRK1 showed improved drought tolerance

Plant Cell Reports, Год журнала: 2023, Номер 42(10), С. 1611 - 1628

Опубликована: Авг. 14, 2023

Язык: Английский

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Isolation, Genome-Wide Identification and Expression Profiling of CYC/TB1 Like Transcription Factor from Saccharum Complex Under Water Deficit Stress Condition

Tropical Plant Biology, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 30, 2025

Язык: Английский

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Isolation, Characterization and Genome-Wide Identification of Cys-2/His-2 Type Zinc Finger Nuclease, a Transcription Activator in Saccharum Complex Under Abiotic Stress Conditions

Sugar Tech, Год журнала: 2024, Номер unknown

Опубликована: Апрель 2, 2024

Язык: Английский

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ZmNF-YB10 , a maize NF-Y transcription factor, positively regulates drought and salt stress response in Arabidopsis thaliana

GM crops & food, Год журнала: 2024, Номер 16(1), С. 28 - 45

Опубликована: Дек. 24, 2024

Maize (Zea mays L.) is a major food and feed crop an important raw material for energy, chemicals, livestock. The NF-Y family of transcription factors in maize plays crucial role the regulation plant development response to environmental stress. In this study, we successfully cloned characterized factor gene ZmNF-YB10. We used bioinformatics, quantitative fluorescence PCR, other techniques analyze basic properties gene, its tissue expression specificity, drought, salt, stresses. results indicated that was 1209 base pairs (bp) length, with coding sequence (CDS) region 618 bp, encoding polypeptide composed 205 amino acid residues. This has theoretical isoelectric point 5.85 features conserved structural domain unique family. Quantitative PCR demonstrated ZmNF-YB10 differentially upregulated under drought salt stress treatments but exhibited negatively regulated pattern alkali cold treatments. Transgenic Arabidopsis thaliana subjected soil showed greener leaves than wild-type A. thaliana. addition, overexpression lines reduced levels hydrogen peroxide (H2O2), superoxide (O2-), malondialdehyde (MDA) increased activities peroxidase (POD), catalase (CAT), dismutase (SOD). Western blot analysis revealed distinct band at 21.8 kDa. Salt tolerance analyses conducted E. coli BL21 positive regulation. yeast cells, biological function enhances tolerance. Protein interactions were observed among ZmNF-YB10, ZmNF-YC2, ZmNF-YC4 genes. It hypothesized genes may play abiotic stresses, such as tolerance, maize.

Язык: Английский

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Identification and expression analysis of nuclear factor Y transcription factor genes under drought, cold and Eldana infestation in sugarcane (Saccharum spp. hybrid)

Genes & Genomics, Год журнала: 2024, Номер 46(8), С. 927 - 940

Опубликована: Июнь 14, 2024

Abstract Background The Nuclear Factor Y (NF-Y) transcription factor (TF) gene family plays a crucial role in plant development and response to stress. Limited information is available on this sugarcane. Objectives To identify sugarcane NF-Y genes through bioinformatic analysis phylogenetic association investigate the expression of these abiotic biotic Methods Sugarcane were identified using comparative genomics from functionally annotated Poaceae Arabidopsis species. Quantitative PCR transcriptome assigned preliminary functional roles water deficit, cold African borer ( Eldana saccharina ) infestation. Results We 21 Phylogenetic revealed three main branches representing subunits with potential discrepancies present assignment numerical names some putative orthologs across different Gene indicated that genes, ShNF-YA1, A3 B3 upregulated two NF-YA4 A7 downregulated, while upregulated, ShNF-YB2, C4, plants exposed deficit stress, respectively. Functional involvement stress also detected where ShNF-YA6, downregulated early resistant (cv. N33) infestation whilst only ShNF-YA6 was susceptible N11) response. Conclusions Our research findings establish foundation for investigating function ShNF-Ys offer candidate stress-resistant breeding improvement

Язык: Английский

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Plant Nuclear Factor Y (NF-Y) Transcription Factors: Evolving Insights into Biological Functions and Gene Expansion

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 38 - 38

Опубликована: Дек. 24, 2024

Gene expansion is a common phenomenon in plant transcription factor families; however, the underlying molecular mechanisms remain elusive. Examples of gene factors are found all eukaryotes. One example nuclear Y (NF-Y) factors. NF-Y ubiquitous to eukaryotes and comprises three independent protein families: NF-YA, NF-YB, NF-YC. While animals fungi mostly have one each subunit, greatly expanded plants. For example, humans NF-YC, while model Arabidopsis has ten Our understanding NF-Y, including its biological roles, mechanisms, expansion, improved over past few years. Here we will review roles focus on studies demonstrating that can serve as for expansion. These show be classified into ancestrally related subclasses. Further, primary structure contains conserved core domain flanked by non-conserved N- C-termini. The C-termini, under pressure diversifying selection, may provide clues this family’s retention functional diversification following duplication. In summary, demonstrates potential used study families.

Язык: Английский

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Populus euphratica CPK21 Interacts with NF-YC3 to Enhance Cadmium Tolerance in Arabidopsis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7214 - 7214

Опубликована: Июнь 29, 2024

The toxic metal cadmium (Cd) poses a serious threat to plant growth and human health. Populus euphratica calcium-dependent protein kinase 21 (CPK21) has previously been shown attenuate Cd toxicity by reducing accumulation, enhancing antioxidant defense improving water balance in transgenic Arabidopsis. Here, we confirmed protein–protein interaction between PeCPK21 Arabidopsis nuclear transcription factor YC3 (AtNF-YC3) yeast two-hybrid bimolecular fluorescence complementation assays. AtNF-YC3 was induced strongly expressed PeCPK21-overexpressed plants. Overexpression of reduced the inhibition root length, fresh weight membrane stability under stress conditions (100 µM, 7 d), suggesting that appears contribute improvement tolerance. improved tolerance limiting uptake activating enzymes hydrogen peroxide (H2O2) production stress. We conclude interacts with limit accumulation enhance reactive oxygen species (ROS) scavenging system thereby positively regulate adaptation environments. This study highlights conditions, which can be utilized improve higher

Язык: Английский

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Overexpression of an NF-YB gene family member, EaNF-YB2, enhances drought tolerance in sugarcane (Saccharum Spp. Hybrid)

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 26, 2024

Drought is one of main critical factors that limits sugarcane productivity and juice quality in tropical regions. The unprecedented changes climate such as monsoon failure, increase temperature other warrant the need for development stress tolerant cultivars to sustain sugar production. Plant Nuclear factor (NF-Y) major classes transcription have a role plant abiotic response. In our previous studies, we found under drought conditions, nuclear NF-YB2 was highly expressed Erianthus arundinaceus, an wild genus Saccharum species. this study, coding sequence gene isolated from arundinaceus overexpressed develop lines. EaNF-YB2 overexpressing (OE) lines had higher relative water content, chlorophyll content photosynthetic efficiency compared non-transgenic (NT) control. addition, activity antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), proline lower malondialdehyde (MDA) peroxide (H2O2) contents. expression studies revealed significantly OE than NT control stress. elevated responsive genes BRICK, HSP 70, DREB2, EDH45, LEA3. morphological analysis exhibited less wilting conditions. This study provides insights into tolerance sugarcane. Based on findings can be potentially exploited produce production deficit

Язык: Английский

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