Assembly and Stability of Virus Like Particles from the Gag Capsid Domain from the Human Endogenous Retrovirus HML‐2 DOI Creative Commons

Alexander S. Hebbelstrup,

Kaare Teilum

ChemistrySelect, Год журнала: 2024, Номер 9(35)

Опубликована: Сен. 16, 2024

Abstract The capsid domain of the Gag protein from human endogenous retrovirus HML‐2 can form virus‐like particles (VLPs) in vitro . Such made proteins other organisms are used as nanocarriers. Repeated use exogeneous VLPs may however lead to an immune response. Recombinantly produced and purified could, given its origin, provide a nanocarrier system, which potentially could evade It is not known if capsids formed by be loaded with molecular cargo such stable. In this work, we investigated how assembly efficiency change conditions. We demonstrate that once stable over much broader range pH than allows formation, for weeks. furthermore show encapsulate small molecules. Our results suggest serve nanocarriers protecting surrounding environment.

Язык: Английский

Species-Specific Transcription Factors Associated with Long Terminal Repeat Promoters of Endogenous Retroviruses: A Comprehensive Review DOI Creative Commons
Md. Jakir Hossain,

Perpetual Nyame,

Kazuaki Monde

и другие.

Biomolecules, Год журнала: 2024, Номер 14(3), С. 280 - 280

Опубликована: Фев. 26, 2024

Endogenous retroviruses (ERVs) became a part of the eukaryotic genome through endogenization millions years ago. Moreover, they have lost their innate capability virulence or replication. Nevertheless, in cells, actively engage various activities that may be advantageous disadvantageous to cells. The mechanisms by which transcription is triggered and implicated cellular processes are complex. Owing diversity expression factors (TFs) cells TF-binding motifs viruses, comprehensibility ERV initiation its impact on functions unclear. Currently, several known related initiation. TFs bind viral long-terminal repeat (LTR) critical initiators. This review discusses shown associate with stimulation across species such as humans, mice, pigs, monkeys, zebrafish, Drosophila, yeast. A comprehensive summary previously reported aid identifying similarities between animal endogenous viruses. an in-depth understanding will assist elucidating physiological roles cell development clarifying relationship retrovirus-associated diseases.

Язык: Английский

Процитировано

4

Immunogenetics of longevity and its association with human endogenous retrovirus K DOI Creative Commons
Lisa M. James,

Apostolos P. Georgopoulos

Frontiers in Aging, Год журнала: 2025, Номер 6

Опубликована: Фев. 4, 2025

Introduction The human immune system is equipped to neutralize and eliminate viruses other foreign antigens via binding of leukocyte antigen (HLA) molecules with epitopes presenting them T cells. HLA highly polymorphic, resulting in subtle differences the groove that influence elimination. Here we tested hypothesis certain alleles may promote longevity by enhanced ability counter virus otherwise contribute morbidity mortality. Methods We utilized high-resolution genotyping characterize apolipoprotein E a large sample (N = 986) participants (469 men, 517 women) ranging age from 24 90+ years old (mean age: 58.10 years) identified 244 occurred sample. Since each individual carries 12 classical (6 Class I II), determined silico median predicted affinity for (across alleles) 13 common (Human Herpes Virus 1 [HHV1], HHV2, HHV3, HHV4, HHV5, HHV6A, HHV6B, HHV7, HHV8, papilloma [HPV], polyoma [JCV], endogenous retrovirus K [HERVK], HERVW). Next, performed stepwise multiple linear regression where participant was dependent variable HLA-virus affinities were independent variables. Results analyses yielded only one statistically significant effect–namely, positive association between HERVK (P 0.005). Furthermore, (9 HLA-I 4 HLA-II) at greater frequency very individuals (age ≥90 as compared younger individuals. Remarkably, those alleles, significantly higher than < 0.001). ApoE genotypes did not differ older groups. Discussion Taken together, results showed HLA-HERVK robust predictor bind high enriched findings present study highlight interactions host immunogenetics exposure on suggest specific response viruses, notably HERVK.

Язык: Английский

Процитировано

0

Human Endogenous Retroviruses as Novel Therapeutic Targets in Neurodegenerative Disorders DOI Creative Commons
Elena Rita Simula, Seyedesomaye Jasemi, Davide Cossu

и другие.

Vaccines, Год журнала: 2025, Номер 13(4), С. 415 - 415

Опубликована: Апрель 15, 2025

Human Endogenous Retroviruses comprise approximately 8% of the human genome, serving as fragments ancient retroviral infections. Although they are generally maintained in a silenced state by robust epigenetic mechanisms, specific HERV groups, particularly HERV-W and HERV-K, can become derepressed under pathological conditions, thereby contributing to initiation progression neuroinflammatory neurodegenerative processes. Preclinical studies clinical trials, such those investigating monoclonal antibodies, indicate that directly targeting these elements may offer novel therapeutic strategy. In this review, we provide an overview HERVs′ biology, examine their role diseases amyotrophic lateral sclerosis, multiple Alzheimer′s disease, Parkinson′s explore prospects, highlighting both challenges potential future research directions needed translate approaches into interventions.

Язык: Английский

Процитировано

0

Development of human endogenous retrovirus type K‐ related treatments for human diseases DOI
Lu Dai,

Jiaojiao Fan,

Zhiqiang Qin

и другие.

Journal of Medical Virology, Год журнала: 2024, Номер 96(3)

Опубликована: Март 1, 2024

Abstract Human endogenous retroviruses (HERVs) constitute approximately 8% of the human genome and have long been regarded as silent passengers within our genomes. However, reactivation HERVs has increasingly linked to a range diseases, particularly HERV‐K (HML‐2) family. Many studies are dedicated elucidating potential role in pathogenicity. While underlying mechanisms require further investigation, targeting transactivation emerges promising avenue for treating including cancer, autoimmune disorders, neurodegenerative conditions, infectious diseases. In this review, we summarize recent advancements development HERV‐K‐targeted therapeutic strategies against various antiretroviral drugs, immunotherapy, vaccines.

Язык: Английский

Процитировано

2

Roles of Human Endogenous Retrovirus-K-Encoded Np9 in Human Diseases: A Small Protein with Big Functions DOI Creative Commons

Jiaojiao Fan,

Zhiqiang Qin

Viruses, Год журнала: 2024, Номер 16(4), С. 581 - 581

Опубликована: Апрель 10, 2024

Human Endogenous Retrovirus Sequences (HERVs) constitute up to 8% of the human genome, yet not all HERVs remain silent passengers within our genomes. Some HERVs, especially HERV type K (HERV-K), have been found be frequently transactivated in a variety inflammatory diseases and cancers. Np9, small protein translated from HERV-K

Язык: Английский

Процитировано

1

Evolution of Repetitive Elements, Their Roles in Homeostasis and Human Disease, and Potential Therapeutic Applications DOI Creative Commons
Jeffrey M. Snowbarger, Praveen Koganti, Charles Spruck

и другие.

Biomolecules, Год журнала: 2024, Номер 14(10), С. 1250 - 1250

Опубликована: Окт. 2, 2024

Repeating sequences of DNA, or repetitive elements (REs), are common features across both prokaryotic and eukaryotic genomes. Unlike many their protein-coding counterparts, the functions REs in host cells remained largely unknown have often been overlooked. While there is still more to learn about functions, now recognized play significant roles beneficial pathological processes hosts at cellular organismal levels. Therefore, this review, we discuss various types review what known evolution. In addition, aim classify general mechanisms by which promote that variously harmful cells/organisms. Finally, address emerging role cancer, aging, neurological disorders provide insights into how RE modulation could new therapeutic benefits for these specific conditions.

Язык: Английский

Процитировано

1

Assembly and Stability of Virus Like Particles from the Gag Capsid Domain from the Human Endogenous Retrovirus HML‐2 DOI Creative Commons

Alexander S. Hebbelstrup,

Kaare Teilum

ChemistrySelect, Год журнала: 2024, Номер 9(35)

Опубликована: Сен. 16, 2024

Abstract The capsid domain of the Gag protein from human endogenous retrovirus HML‐2 can form virus‐like particles (VLPs) in vitro . Such made proteins other organisms are used as nanocarriers. Repeated use exogeneous VLPs may however lead to an immune response. Recombinantly produced and purified could, given its origin, provide a nanocarrier system, which potentially could evade It is not known if capsids formed by be loaded with molecular cargo such stable. In this work, we investigated how assembly efficiency change conditions. We demonstrate that once stable over much broader range pH than allows formation, for weeks. furthermore show encapsulate small molecules. Our results suggest serve nanocarriers protecting surrounding environment.

Язык: Английский

Процитировано

0