Retinal Organoids: Advances in Generation, Development, and Applications – From Stem Cells to Disease Modeling and Regenerative Medicine
IntechOpen eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 31, 2025
Retinal
diseases,
often
caused
by
the
degeneration
of
light-sensitive
cells,
pose
a
significant
global
health
challenge.
While
current
treatments
are
limited,
cell
therapy,
involving
transplantation
healthy
offers
hope.
Recent
advancements
in
stem
technology
have
enabled
creation
3D
retinal
organoids,
which
closely
mimic
structure
and
function
human
retina.
These
organoids
provide
valuable
platform
for
studying
development,
modeling
testing
potential
therapies.
By
understanding
molecular
mechanisms
underlying
eye
formation
disease
progression,
researchers
can
identify
develop
targeted
treatments.
hold
promise
revolutionizing
field
regenerative
medicine
vision
research,
offering
hope
restoring
improving
lives
individuals
affected
diseases.
Язык: Английский
Retinal Organoids: Innovative Tools for Understanding Retinal Degeneration
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3263 - 3263
Опубликована: Апрель 1, 2025
Retinal
degenerative
diseases
(RDDs)
comprise
diverse
genetic
and
phenotypic
conditions
that
cause
progressive
retinal
dysfunction
cell
loss,
leading
to
vision
impairment
or
blindness.
Most
RDDs
lack
appropriate
animal
models
for
their
study,
which
affects
understanding
disease
mechanisms
delays
the
progress
of
new
treatment
development.
Recent
advances
in
stem
engineering,
omics,
organoid
technology
are
facilitating
research
into
there
no
previously
existing
models.
The
development
organoids
produced
from
human
cells
has
impacted
study
as
well
vitro
diseases,
opening
possibilities
applications
regenerative
medicine,
drug
discovery,
precision
medicine.
In
this
review,
we
recapitulate
RDD,
mentioning
some
main
pathways
underlying
neurodegeneration
can
be
studied
these
models,
limitations
future
challenges
rapidly
advancing
field.
Язык: Английский
Magnetic‐Guided Delivery of Antisense Oligonucleotides for Targeted Transduction in Multiple Retinal Explant and Organoid Models
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
Antisense
oligonucleotide
(ASO)
therapy
holds
promise
in
gene
but
faces
challenges
due
to
poor
delivery
efficiency
and
limited
evaluation
models.
This
investigation
employs
magnetic
nanoparticles
(MNPs)
augment
the
of
ASOs.
It
assesses
their
distribution
therapeutic
efficacy
across
various
models,
including
retinal
explants
from
mice
macaques
or
human
inner
ear
organoids.
Retinal
both
monkeys
are
methodically
arranged
expose
ganglion
cell
layer
(GCL)
photoreceptor
(PL).
MNPs
markedly
enhanced
penetration
targeting
ASOs,
resulting
a
60%
accumulation
GCL
72%
photoreceptors.
Furthermore,
an
vitro
biomimetic
model
neuroretina‐RPE/choroid‐sclera
complex
is
developed
examine
ASO
under
dynamic
flow
conditions.
Moreover,
utilization
MNP‐assisted
ASO‐Cy3
transfection
within
organoids,
increase
positively
transfected
cells
70%,
respectively.
Here,
for
first
time,
MNP‐explant‐organoid
platform
carried
out
promotion
efficiency,
screening
targeted
delivery.
development
paves
way
investigating
novel
strategies
diseases.
Язык: Английский