Cloning and expression analysis of RhHsf24 gene in Rose (Rosa hybrida)
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 10, 2025
Rose
(Rosa
hybrida)
is
one
of
the
most
important
ornamental
and
perfume
industry
crops
worldwide,
both
economically
culturally.
Abiotic
stresses,
such
as
high
temperature
salt
are
crucial
factors
influencing
quality
roses.
In
this
study,
RhHsf24
was
isolated
from
rose
(R.
hybrida
'Samantha'),
which
encodes
295
amino
acids
(aa).
Sequence
comparison
with
members
Arabidopsis
Hsfs
family
revealed
that
gene
closely
related
to
AtHsfB1;
phylogenetic
tree
analysis
proteins
other
species
showed
it
clusters
R.
rugosa
(RrHSF24),
Fragaria
vesca
(FvHSFB1a)
Argentina
anserina
(AaHSF24),
closest
relatives
belong
class
B
heat
shock
transcription
factors.
localized
in
nucleus.
The
qRT-PCR
results
indicated
expressed
roots,
stems,
leaves,
flowers
buds.
Expression
leaves
subjected
various
temperatures
durations
stress
treatment
demonstrated
expression
induced
by
stress.
Under
stress,
generally
exhibited
a
level
increasing
concentration.
above
preliminarily
clarified
biological
function
RhHsf24,
provide
genetic
resource
theoretical
reference
for
resistance
breeding
Язык: Английский
Functional Analysis of CsHsf10 in Drought Stress Response in Camellia sinensis
Horticulturae,
Год журнала:
2025,
Номер
11(4), С. 373 - 373
Опубликована: Март 31, 2025
Heat
shock
transcription
factor
(Hsf)
plays
a
crucial
role
in
the
signal
transduction
pathways
of
plants
response
to
drought
stress.
However,
studies
exploring
specific
functions
and
mechanisms
action
Hsf
family
tea
(Camellia
sinensis
L.)
remain
limited.
In
this
study,
we
identified
31
members
CsHsf
from
C.
genome.
CsHsf10
was
determined
be
potential
drought-resistant
candidate
gene
by
screening
10
highly
expressed
genes
mature
leaves
confirming
results
through
RT-qPCR.
Correlation
analysis
indicates
that
may
enhance
resistance
participating
polyphenol
synthesis
pathway
regulating
expression
antioxidant
enzyme
genes.
Furthermore,
overexpression
experiments
Arabidopsis
antisense
oligonucleotide
corroborated
exerts
significant
positive
regulatory
effect
on
plants.
Yeast
one-hybrid
assays
dual
luciferase
reporter
demonstrated
can
directly
target
CsPOD17,
significantly
promoting
its
transcriptional
expression.
Additionally,
found
contributes
increased
accumulation
catechin
components
under
These
findings
suggest
that,
during
stress,
not
only
enhances
capacity
activity
enzymes
but
also
optimizes
physiological
state
influencing
secondary
metabolites,
thereby
improving
their
resistance.
Язык: Английский