Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Ноя. 15, 2023
This
study
addresses
the
environmental
risks
associated
with
accumulation
of
keratin
waste
from
poultry,
which
is
resistant
to
conventional
protein
degradation
methods.
To
tackle
this
issue,
microbial
keratinases
have
emerged
as
promising
tools
for
transforming
resilient
materials
into
valuable
products.
We
focus
on
Metalloprotease
(MetPr)
gene
isolated
novel
Pichia
kudriavzevii
YK46,
sequenced,
and
deposited
in
NCBI
GenBank
database
accession
number
OQ511281.
The
MetPr
encodes
a
consisting
557
amino
acids
demonstrates
keratinase
activity
164.04
U/ml.
3D
structure
was
validated
using
Ramachandran's
plot,
revealing
that
93%
97.26%
residues
were
situated
within
most
favoured
region
proteins
template
strain
129
respectively.
Computational
analyses
employed
determine
binding
affinities
between
deduced
beta
keratin.
Molecular
docking
studies
elucidated
optimal
metalloprotease
beta-keratin,
yielding
values
-
260.75
kcal/mol
257.02
strains
Subsequent
molecular
cloning
expression
E.
coli
DH5α
led
significantly
higher
281
±
12.34
These
findings
provide
insights
potential
its
encoded
biotransformation,
implications
addressing
concerns
related
keratinous
accumulation.
ACS Omega,
Год журнала:
2025,
Номер
10(12), С. 12224 - 12232
Опубликована: Март 19, 2025
Hydrolyzed
keratin
(HK)
refers
to
any
hydrolysate
of
a
different
origin
derived
by
acid,
alkali,
enzymatic,
or
other
methods
hydrolysis.
HK
is
water
soluble
and
has
distinct
chemical–physical
properties
compared
fibrous
keratin.
Although
employed
across
various
technological
sectors,
there
notable
gap
in
the
literature
regarding
detailed
commercially
available
HKs.
This
study
aims
address
this
providing
thorough
analysis
surface-active,
emulsifying,
thickening
three
The
results
reveal
relevant
differences
among
HKs
marketed
under
International
Nomenclature
Cosmetic
Ingredient
(INCI)
name
"Hydrolyzed
Keratin,"
with
variations
their
properties,
primarily
influenced
molecular
weight.
Specifically,
higher
average
Mw
(>3000
Da)
protein
content
demonstrate
enhanced
emulsifying
capabilities.
Conversely,
low
(<1000
do
not
show
surface-active
suitable
for
preparation
emulsions.
Therefore,
underscores
need
standardization
products
obtain
biomaterials
tailored
specific
enhancing
potential
applications
cosmetic
pharmaceutical
topical
formulations.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 16, 2025
Keratin,
one
of
the
most
recalcitrant
and
abundant
proteins
on
Earth,
constitutes
a
challenging
underutilized
material
for
poultry
industry.
Although
it
resists
degradation
by
commonly
available
enzymes,
natural
breakdown
occurs
through
action
certain
fungi
bacteria.
This
process
remains
poorly
understood,
only
few
thermophilic
anaerobic
bacteria
are
known
to
effectively
degrade
keratin.
Some
members
genus
Fervidobacterium
have
been
demonstrated
be
effective
at
degrading
feather
keratin
under
high
temperatures
anoxic
conditions.
However,
comprehensive
evaluation
their
keratinolytic
capabilities
lacking,
leaving
potential
largely
underexplored.
In
this
study,
we
assessed
activity
all
strains.
Six
strains
were
active
against
substrate,
namely
changbaicum
CBS-1T,
islandicum
H-21T,
pennivorans
T,
DSM9078T,
sp.
GSH,
21710.
These
used
in
comparative
proteomics
analysis,
grown
with
either
glucose
or
chicken
feathers
as
sole
carbon
source.
Similarly,
three
efficient
strains,
H-21T
underwent
an
in-depth
transcriptomics
analysis.
Among
numerous
upregulated
overexpressed
genes
identified
when
comparing
feather-grown
glucose-grown
cells,
oxidoreductases
peptidases
key
enzymes
process,
suggesting
application
enzymatic
cocktails
