Towards a comprehensive view of wetland benthic communities

Current Research in Microbial Sciences, Год журнала: 2025, Номер unknown, С. 100391 - 100391

Опубликована: Апрель 1, 2025

Benthic prokaryotic communities, utmost important for wetlands and marine environments functioning, are influenced by physical-chemical parameters interactions with other especially micro-eukaryotes meiofauna. Thus, a holistic view of the benthic community is necessary to fully understand their organization functioning. This study assesses implementation comprehensive view, using mock communities environmental samples. A DNA extraction strategy combining two procedures proposed: one obtain from micro-organisms, 0.25 g sediment, meiofauna, sieving refluxes 5 sediment. Three conditions were considered create communities: (i) varying eukaryotes' abundance, (ii) adding meiofauna salted or freshwater wetlands, (iii) including not sediment matrix. Most organisms composing detected, except filamentous cyanobacteria. All showed similar composition indicating that did affect extraction. result also demonstrated sieving, enrich does significantly any communities. For samples investigated, most taxa usually described in literature retrieved salted, brackish marshes The proposed approach was successful analysing three domains life unique sample, providing community. Furthermore, significant differences observed between different marshes, indicated our can be used conducting ecological studies, useful understanding communities' interactions.

Язык: Английский

The trophic interaction of freshwater chironomids using DNA metabarcoding and stable isotope analysis in stream ecosystem

Journal of Environmental Management, Год журнала: 2025, Номер 383, С. 125479 - 125479

Опубликована: Апрель 24, 2025

Язык: Английский