Skeletal muscle gene expression dysregulation in long-term spaceflights and aging is clock-dependent

npj Microgravity, Год журнала: 2023, Номер 9(1)

Опубликована: Апрель 3, 2023

The circadian clock regulates cellular and molecular processes in mammals across all tissues including skeletal muscle, one of the largest organs human body. Dysregulated rhythms are characteristic aging crewed spaceflight, associated with, for example, musculoskeletal atrophy. Molecular insights into spaceflight-related alterations regulation muscle still missing. Here, we investigated potential functional consequences disruptions on using published omics datasets obtained from spaceflights other clock-altering, external (fasting exercise), or internal (aging) conditions Earth. Our analysis identified network muscle-associated pathways, as a result spaceflight duration mice, which resembles aging-related gene expression changes observed humans Earth (e.g., ATF4 downregulation, with atrophy). Furthermore, according to our results, factors such exercise fasting lead core-clock network, may compensate disruption during spaceflights. Thus, maintaining functioning is crucial ameliorate unphysiological atrophy reported among astronauts.

Язык: Английский

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Inspiring Tactics with the Improvement of Mitophagy and Redox Balance for the Development of Innovative Treatment against Polycystic Kidney Disease

Biomolecules, Год журнала: 2024, Номер 14(2), С. 207 - 207

Опубликована: Фев. 9, 2024

Polycystic kidney disease (PKD) is the most common genetic form of chronic (CKD), and it involves development multiple cysts. Not enough medical breakthroughs have been made against PKD, a condition which features regional hypoxia activation hypoxia-inducible factor (HIF) pathway. The following pathology CKD can severely instigate damage and/or renal failure. Significant evidence verifies an imperative role for mitophagy in normal physiology PKD. Mitophagy serves as important component mitochondrial quality control by removing impaired/dysfunctional mitochondria from cell to warrant redox homeostasis sustain viability. Interestingly, treatment with peroxisome proliferator-activated receptor-α (PPAR-α) agonist could reduce PDK might improve function via modulation mitophagy, well gut microbiome. Suitable be favorable tactic prevention diseases such PKD CKD.

Язык: Английский

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The potential of therapeutic strategies targeting mitochondrial biogenesis for the treatment of insulin resistance and type 2 diabetes mellitus

Archives of Pharmacal Research, Год журнала: 2024, Номер 47(3), С. 219 - 248

Опубликована: Март 1, 2024

Язык: Английский

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Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting PPARγ/miR-29b

Journal of sport and health science/Journal of Sport and Health Science, Год журнала: 2021, Номер 11(6), С. 696 - 707

Опубликована: Июнь 8, 2021

Exercise is beneficial for muscle atrophy. Peroxisome proliferator-activated receptor gamma (PPARγ) and microRNA-29b (miR-29b) have been reported to be responsible angiotensinⅡ (AngⅡ)-induced However, it unclear whether exercise can protect AngⅡ-induced atrophy by targeting PPARγ/miR-29b. Skeletal in both the control group run was established AngⅡ infusion; after 1 week of training, mice were sacrificed, weight determined. Myofiber size measured hematoxylin-eosin wheat-germ agglutinin staining. Apoptosis evaluated terminal deoxynucleotidyl transferase dUTP nick end labeling The expression level atrogenes, including F-box only protein 32 (FBXO32, also called Atrogin-1) muscle-specific RING-finger (MuRF-1), phosphorylation kinase B (PKB, AKT)/forkhead box O3A (FOXO3A)/mammalian target rapamycin (mTOR) pathway proteins, PPARγ apoptosis-related B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), cysteine-aspartic acid protease 3 (caspase-3), cleaved-caspase-3, determined western blot. miR-29b checked reverse-transcription quantitative polymerase chain reaction. A inhibitor (T0070907) or adeno-associated virus serotype-8 (AAV8)-mediated overexpression used demonstrate activation inhibition mediates effects significantly attenuate atrophy, which demonstrated increased skeletal weight, cross-sectional area myofiber, AKT/mTOR signaling decreased atrogenes expressions apoptosis. In models, elevated whereas exercise. protective inhibited a overexpression. attenuates suppression miR-29b.

Язык: Английский

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A Pound of Flesh: What Cachexia Is and What It Is Not

Diagnostics, Год журнала: 2021, Номер 11(1), С. 116 - 116

Опубликована: Янв. 12, 2021

Body weight loss, mostly due to the wasting of skeletal muscle and adipose tissue, is hallmark so-called cachexia syndrome. Cachexia associated with several acute chronic disease states such as cancer, obstructive pulmonary (COPD), heart kidney failure, acquired autoimmune diseases also pharmacological treatments chemotherapy. The clinical relevance its impact on patients’ quality life has been neglected for decades. Only recently did international community agree upon a definition term cachexia, we are still awaiting standardization markers tests diagnosis staging cancer-related cachexia. In this review, discuss considering evolving use diagnostic purposes implications it biomarkers, provide comprehensive overview biology management. Advances tools developed so far in vitro testing drug screening will be described. We evaluate nomenclature different forms degeneration features that distinguish from other context conditions.

Язык: Английский

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Identification of Key Genes and Biological Pathways Associated with Skeletal Muscle Maturation and Hypertrophy in Bos taurus, Ovis aries, and Sus scrofa

Animals, Год журнала: 2022, Номер 12(24), С. 3471 - 3471

Опубликована: Дек. 8, 2022

The aim of the current study was to identify major genes and pathways involved in process hypertrophy skeletal muscle maturation that is common for Bos taurus, Ovis aries, Sus scrofa species. Gene expression profiles related muscle, with accession numbers GSE44030, GSE23563, GSE38518, respectively, were downloaded from GEO database. Differentially expressed (DEGs) screened out using Limma package R software. Genes Fold Change > 2 an adjusted p-value < 0.05 identified as significantly different between two treatments each Subsequently, gene ontology pathway enrichment analyses performed. Moreover, hub detected by creating a protein−protein interaction network (PPI). results analysis taurus showed period 280 dpc−3-months old, total 1839 significant difference. In however, during 135dpc−2-months 486 different. Additionally, 91 dpc−adult period, 2949 scrofa. KEGG GO function annotation species separately revealed DEGs mainly enriched through fiber development contraction, positive regulation fibroblast proliferation, development, PPAR signaling pathway, HIF-1 pathway. regulating cell growth, differentiation, PI3K-Akt signaling, Rap1 pathways. scrofa, striated tissue negative proliferation myoblast AMPK Using Venn diagram, 36 A biological allowed identification pathways/biological processes, such orchestrated muscle. As result, identified, including PPARGC1A, MYOD1, EPAS1, IGF2, CXCR4, APOA1, all examined This provided better understanding relationships their process.

Язык: Английский

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Integrated design and fabrication strategies based on bioprinting for skeletal muscle regeneration: Current status and future perspectives

Materials & Design, Год журнала: 2023, Номер 225, С. 111591 - 111591

Опубликована: Янв. 1, 2023

The number of skeletal muscle injuries derived from myopathies, exercise, and trauma, is growing due to increasing sports activities in normal life people. Skeletal has a robust capacity for regeneration following injury. However, few if any effective therapeutic options volumetric loss are available. Poor repair injury will lead dysfunction, resulting physical pain even seriously compromising daily life. As new technology biomedicine, 3D bioprinting widely used the field tissue engineering regenerative medicine its enormous advantages. creates biological structures similar that protists by precisely constructing specified geometric shape at micro/nano levels, thus addressing unmet needs replacement organ transplantation. With further application various repairs, more researchers utilized regeneration. Various bioinks, such as alginate, gelatin, fibrin hydrogels, extracellular matrix (ECM), loaded with functional seed cells or growth factors have been applied fabricating bioprinted constructs complicated inner Therefore, we conducted this review report process, development status technology, mechanism promoting repair. In addition, current challenges perspectives also discussed study.

Язык: Английский

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Effects of Probiotics Supplementation on the Intestinal Metabolites, Muscle Fiber Properties, and Meat Quality of Sunit Lamb

Animals, Год журнала: 2023, Номер 13(4), С. 762 - 762

Опубликована: Фев. 20, 2023

The development of animal husbandry is closely related to the meat quality small ruminants. Intestinal metabolites and muscle fiber types lambs are important factors that affect their quality, but few studies have examined regulation "intestinal axis" by probiotics. In this study, 12 Sunit were divided into a control group (C) probiotics (P). gene expressions myosin heavy chain, metabolic enzyme activity, short-chain fatty acids in intestines analyzed using gas chromatography-mass spectrometry (GC-MS) quantitative real-time PCR. results showed levels propionic acid butyric P significantly higher than C (p < 0.05). addition, increased number area ratio type I fibers. They also mRNA expression MyHC IIA activity malate dehydrogenase (MDH) succinate (SDH). Propionic was negatively correlated with IIB Butyric found be positively Cooking loss, pH24h, shear force decreased P. conclusion, intestinal (SCFAs) altered oxidative-myofibril-metabolizing enzymes heavy-chain IIA, reduced values, improved tenderness. This study provides new basis for improving production

Язык: Английский

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Exploring the Gut Microbiota–Muscle Axis in Duchenne Muscular Dystrophy

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 5589 - 5589

Опубликована: Май 21, 2024

The gut microbiota plays a pivotal role in maintaining the dynamic balance of intestinal epithelial and immune cells, crucial for overall organ homeostasis. Dysfunctions these intricate relationships can lead to inflammation contribute pathogenesis various diseases. Recent findings uncovered existence gut–muscle axis, revealing how alterations disrupt regulatory mechanisms muscular adipose tissues, triggering immune-mediated inflammation. In context Duchenne dystrophy (DMD), permeability stand as potential origin molecules that could trigger muscle degeneration via pathways. Metabolites produced by bacteria, or fragments bacteria themselves, may have ability migrate from into bloodstream ultimately infiltrate distant exacerbating localized pathologies. These insights highlight alternative pathological pathways DMD beyond musculoskeletal system, paving way nutraceutical supplementation adjuvant therapy. Understanding complex interplay between microbiota, health offers new perspectives therapeutic interventions conventional approaches efficiently counteract multifaceted nature DMD.

Язык: Английский

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PPARs in Clinical Experimental Medicine after 35 Years of Worldwide Scientific Investigations and Medical Experiments

Biomolecules, Год журнала: 2024, Номер 14(7), С. 786 - 786

Опубликована: Июль 1, 2024

This year marks the 35th anniversary of Professor Walter Wahli's discovery PPARs (Peroxisome Proliferator-Activated Receptors) family nuclear hormone receptors. To mark occasion, editors scientific periodical Biomolecules decided to publish a special issue in his honor. paper summarizes what is known about and shows how trends have changed research on has evolved. The article also highlights importance role they play various diseases ailments. mixed form; essentially it review article, but been enriched with results our experiments. selection works was subjective, as there are more than 200,000 publications PubMed database alone. First, all papers done an animal model were discarded at outset. What remained still far too large describe directly. Therefore, only that outstanding, groundbreaking, or simply interesting described briefly commented on.

Язык: Английский

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