Diagnostics,
Год журнала:
2022,
Номер
12(7), С. 1524 - 1524
Опубликована: Июнь 23, 2022
Hepatitis
C
virus
(HCV)
infection
can
be
cured
with
pan-genotypic
direct-acting
antiviral
agents.
However,
identifying
individuals
current
hepatitis
remains
a
major
challenge,
especially
in
resource-limited
settings
where
access
to
or
availability
of
molecular
tests
is
still
limited.
The
goal
this
study
was
develop
and
validate
assay
for
the
rapid
detection
HCV
RNA
settings.
It
based
on
combination
reverse
transcription
loop-mediated
isothermal
amplification
(RT-LAMP)
clustered
regularly
interspaced
short
palindromic
repeats–CRISPR-associated
protein
12a
(CRISPR–Cas12a)
cleavage
that
allows
recognition
specific
nucleic
acid
sequences.
Amplified
products
after
reactions
visualized
lateral
flow
strips
measured
fluorescence
detector.
When
tested
clinical
samples
from
infected
HCV,
HIV,
HBV,
healthy
donors,
RT-LAMP-coupled
CRISPR–Cas12
yielded
96%
sensitivity,
100%
specificity,
97%
agreement
as
compared
reference
method
(Roche
COBAS
AmpliPrep/COBAS
TaqMan
Test).
This
could
detect
concentrations
low
10
ng/µL
(an
estimated
2.38
Log10
IU/mL).
Therefore,
sensitive
may
represent
an
affordable
reliable
point-of-care
test
identification
active
low-resource
Frontiers in Genetics,
Год журнала:
2022,
Номер
13
Опубликована: Апрель 26, 2022
Over
the
last
decade,
remarkable
progress
has
been
made
in
our
understanding
phytohormones,
cytokinin’s
(CKs)
biosynthesis,
perception,
and
signalling
pathways.
Additionally,
it
became
apparent
that
interfering
with
any
of
these
steps
a
significant
effect
on
all
stages
plant
growth
development.
As
result
their
complex
regulatory
cross-talk
interactions
other
hormones
networks,
they
influence
control
wide
range
biological
activities,
from
cellular
to
organismal
levels.
In
agriculture,
CKs
are
extensively
used
for
yield
improvement
management
because
wide-ranging
effects
growth,
development
physiology.
One
primary
targets
this
regard
is
cytokinin
oxidase/dehydrogenase
(CKO/CKX),
which
encoded
by
CKX
gene,
catalyses
irreversible
degradation
cytokinin.
The
previous
studies
various
agronomically
important
crops
indicated
breeders
have
targeted
directly.
recent
years,
prokaryotic
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
protein
9
(Cas9)
system
increasingly
editing
CKO/CKX
gene
phenomenal
results
achieved.
This
review
provides
an
updated
information
applications
CRISPR-based
gene-editing
tools
manipulating
metabolism
at
genetic
level
improvement.
Furthermore,
we
summarized
current
developments
RNP-mediated
DNA/transgene-free
genomic
plants
would
broaden
application
technology.
will
advance
cytokinins
role
sustainably
increase
crop
production
through
CRISPR/Cas
genome
tool.
Diagnostics,
Год журнала:
2022,
Номер
12(7), С. 1524 - 1524
Опубликована: Июнь 23, 2022
Hepatitis
C
virus
(HCV)
infection
can
be
cured
with
pan-genotypic
direct-acting
antiviral
agents.
However,
identifying
individuals
current
hepatitis
remains
a
major
challenge,
especially
in
resource-limited
settings
where
access
to
or
availability
of
molecular
tests
is
still
limited.
The
goal
this
study
was
develop
and
validate
assay
for
the
rapid
detection
HCV
RNA
settings.
It
based
on
combination
reverse
transcription
loop-mediated
isothermal
amplification
(RT-LAMP)
clustered
regularly
interspaced
short
palindromic
repeats–CRISPR-associated
protein
12a
(CRISPR–Cas12a)
cleavage
that
allows
recognition
specific
nucleic
acid
sequences.
Amplified
products
after
reactions
visualized
lateral
flow
strips
measured
fluorescence
detector.
When
tested
clinical
samples
from
infected
HCV,
HIV,
HBV,
healthy
donors,
RT-LAMP-coupled
CRISPR–Cas12
yielded
96%
sensitivity,
100%
specificity,
97%
agreement
as
compared
reference
method
(Roche
COBAS
AmpliPrep/COBAS
TaqMan
Test).
This
could
detect
concentrations
low
10
ng/µL
(an
estimated
2.38
Log10
IU/mL).
Therefore,
sensitive
may
represent
an
affordable
reliable
point-of-care
test
identification
active
low-resource
