Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(10), С. 10846 - 10853
Опубликована: Сен. 27, 2024
Major
depressive
disorder
(MDD)
is
a
complex
mental
health
condition
with
multifaceted
and
incompletely
elucidated
pathophysiology.
MicroRNAs
(miRNAs)
have
emerged
as
potential
biomarkers
due
to
their
role
in
gene
regulation
the
observed
dysregulation
MDD.
The
aim
of
this
study
detect
presence
specific
molecular
diagnostic
major
disorder.
This
cross-sectional
analyzed
plasma
miRNA
expression
ten
MDD
patients
eight
healthy
controls
using
real-time
PCR.
Differentially
expressed
miRNAs
were
identified
independent
t-tests,
was
assessed
ROC
curve
analysis.
Fifteen
exhibited
significant
patients.
Notably,
hsa-miR-29c-3p,
hsa-miR-376a-3p,
hsa-miR-532-5p,
hsa-miR-339-5p
showed
excellent
discriminatory
power
(AUC
>
0.8).
identifies
differentially
MDD,
suggesting
for
improved
diagnosis
personalized
treatment.
However,
further
validation
larger
cohorts
investigation
into
functional
roles
are
warranted.
Endocrine Metabolic & Immune Disorders - Drug Targets,
Год журнала:
2024,
Номер
24(10), С. 1131 - 1145
Опубликована: Фев. 2, 2024
:
Inflammation,
demyelination,
and
neurodegeneration
are
symptoms
of
the
central
nervous
system
(CNS)
condition
known
as
Multiple
sclerosis
(MS).
Due
to
its
crucial
function
in
controlling
immune
cell
activation
inflammation,
glycogen
synthase
kinase-3β
(GSK-
3β),
Bruton's
tyrosine
kinase
(BTK),
Sphingosine
1
phosphate
(S1P)
signaling
pathway
have
become
a
viable
target
for
therapy
MS.
The
GSK-3β
system,
which
controls
several
biological
processes,
including
survival,
proliferation,
depends
on
enzyme.
In
MS
animal
models
human
studies,
inhibition
has
been
demonstrated
lessen
demyelination
inflammation.
Clinical
research
that
BTK
inhibitors
decrease
inflammation
disease
activity
by
preventing
B
subsequent
release
cytokines.
investigations
S1P
modulators,
such
fingolimod,
lower
limiting
migration
cytokine
production.
/BTK/S1P
is
subject
this
paper's
summary
discussion
prospective
treatment
targets.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 23, 2024
Abstract
It
has
been
well-validated
that
chronic
psychological
stress
leads
to
bone
loss,
but
the
underlying
mechanism
remains
unclarified.
In
this
study,
we
established
and
analyzed
unpredictable
mild
(CUMS)
mice
investigate
miRNA-related
pathogenic
involved
in
induced
osteoporosis.
Our
result
found
these
CUMS
exhibited
osteoporosis
phenotype
mainly
attributed
abnormal
activities
of
osteoclasts.
Subsequently,
miRNA
sequencing
other
analysis
showed
miR-335-3p,
which
is
normally
highly
expressed
brain,
was
significantly
down-regulated
nucleus
ambiguous
(NAC),
serum,
mice.
Additionally,
vitro
studies
detected
miR-335-3p
important
for
osteoclast
differentiation,
with
its
direct
targeting
site
FOS.
Further
demonstrated
FOS
upregulated
osteoclast,
inhibition
suppressed
accelerated
osteoclastic
as
well
expression
genes,
such
Nfatc1,
Acp5,
Mmp9
,
restrained
conclusion,
work
indicated
may
down-regulate
expression,
resulted
accumulation
up-regulation
NFACT1
signaling
pathway
osteoclasts,
leading
differentiation
activity.
These
results
decipher
a
previously
unrecognized
paradigm
can
act
link
between
metabolism.
Impact
statement
targets
inhibits
activation
NFATC1
signaling,
an
regulator
function
responsible
It
has
been
well-validated
that
chronic
psychological
stress
leads
to
bone
loss,
but
the
underlying
mechanism
remains
unclarified.
In
this
study,
we
established
and
analyzed
unpredictable
mild
(CUMS)
mice
investigate
miRNA-related
pathogenic
involved
in
stress-induced
osteoporosis.
Our
result
found
these
CUMS
exhibited
osteoporosis
phenotype
mainly
attributed
abnormal
activities
of
osteoclasts.
Subsequently,
miRNA
sequencing
other
analysis
showed
miR-335-3p,
which
is
normally
highly
expressed
brain,
was
significantly
down-regulated
nucleus
ambiguous
(NAC),
serum,
mice.
Additionally,
vitro
studies
detected
miR-335-3p
important
for
osteoclast
differentiation,
with
its
direct
targeting
site
Fos.
Further
demonstrated
Fos
upregulated
osteoclast,
inhibition
suppressed
accelerated
osteoclastic
as
well
expression
genes,
such
Nfatc1,
Acp5,
Mmp9,
restrained
conclusion,
work
indicated
may
down-regulate
expression,
resulted
accumulation
up-regulation
NFACT1
signaling
pathway
osteoclasts,
leading
differentiation
activity.
These
results
decipher
a
previously
unrecognized
paradigm
can
act
link
between
metabolism.miR-335-3p,
targets
FOS
inhibits
activation
NFATC1
signaling,
an
regulator
function
responsible
induced
Jundishapur Journal of Natural Pharmaceutical Products,
Год журнала:
2024,
Номер
19(3)
Опубликована: Май 28, 2024
Background:
Demyelination
and
inflammation
are
the
most
common
pathobiological
manifestations
contributing
to
depression
in
multiple
sclerosis
(MS).
Objectives:
The
current
study
aimed
evaluate
effects
of
acetyl-L-carnitine
(ALC)
attenuating
depressive-like
symptoms
cuprizone
intoxication
mouse
model.
Methods:
C57BL/6
mice
were
categorized
into
three
groups
(n
=
6
each)
as
follows:
control
animals
(CTL),
cuprizone-intoxicated
(CPZ),
group
that
received
(CPZ+ALC).
Depressive-like
behaviors
evaluated
by
forced
swim
test
(FST)
tail
suspension
(TST).
prefrontal
cortex
(PFC)
corpus
callosum
(CC)
areas
assessed
terms
histopathology,
biochemistry,
gene
expression.
Results:
Following
oral
gavage
ALC,
immobility
time,
which
represents
despairing
significantly
decreased
compared
CPZ
group.
Histopathological
evaluation
showed
remyelination
CC
increased
receiving
ALC
mice.
Acetyl-L-carnitine
considerably
nitric
oxide
(NO)
level
PFC
brain
demyelinated
qRT-PCR
results
revealed
neuronal
synthase
(nNOS)
expression
but
inducible
(iNOS)
Conclusions:
attenuated
demyelination
model
MS.
These
neuroprotective
may
be
exerted
facilitating
process
modulating
NO
PFC.
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(10), С. 10846 - 10853
Опубликована: Сен. 27, 2024
Major
depressive
disorder
(MDD)
is
a
complex
mental
health
condition
with
multifaceted
and
incompletely
elucidated
pathophysiology.
MicroRNAs
(miRNAs)
have
emerged
as
potential
biomarkers
due
to
their
role
in
gene
regulation
the
observed
dysregulation
MDD.
The
aim
of
this
study
detect
presence
specific
molecular
diagnostic
major
disorder.
This
cross-sectional
analyzed
plasma
miRNA
expression
ten
MDD
patients
eight
healthy
controls
using
real-time
PCR.
Differentially
expressed
miRNAs
were
identified
independent
t-tests,
was
assessed
ROC
curve
analysis.
Fifteen
exhibited
significant
patients.
Notably,
hsa-miR-29c-3p,
hsa-miR-376a-3p,
hsa-miR-532-5p,
hsa-miR-339-5p
showed
excellent
discriminatory
power
(AUC
>
0.8).
identifies
differentially
MDD,
suggesting
for
improved
diagnosis
personalized
treatment.
However,
further
validation
larger
cohorts
investigation
into
functional
roles
are
warranted.
