Genes,
Год журнала:
2024,
Номер
15(12), С. 1599 - 1599
Опубликована: Дек. 14, 2024
Background/Objectives:
Aging
is
a
natural
physiological
process
involving
biological
and
genetic
pathways.
Growing
evidence
suggests
that
alterations
in
the
epigenome
during
aging
result
transcriptional
changes,
which
play
significant
role
onset
of
age-related
diseases,
including
cancer,
cardiovascular
disease,
diabetes,
neurodegenerative
disorders.
For
this
reason,
epigenetic
diseases
have
been
reviewed,
major
extrinsic
factors
influencing
these
identified.
In
addition,
gut
microbiome
its
metabolites
as
modifiers
has
addressed.
Results:
Long-term
exposure
to
such
air
pollution,
diet,
drug
use,
environmental
chemicals,
microbial
infections,
physical
activity,
radiation,
stress
provoke
changes
host
through
several
endocrine
immune
pathways,
potentially
accelerating
process.
Diverse
studies
reported
plays
critical
regulating
brain
cell
functions
DNA
methylation
histone
modifications.
The
interaction
between
genes
serves
source
adaptive
variation,
contributing
phenotypic
plasticity.
However,
molecular
mechanisms
signaling
pathways
driving
are
still
not
fully
understood.
Conclusions:
Extrinsic
potential
inducers
alterations,
may
important
implications
for
longevity.
an
effector
gene
expression
modifications,
while
bidirectional
interactions
with
underexplored
roles
non-bacterial
microorganisms
fungi
viruses
highlight
need
further
research.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(35), С. 11967 - 11972
Опубликована: Авг. 23, 2022
One
of
the
main
challenges
in
cancer
management
relates
to
discovery
reliable
biomarkers,
which
could
guide
decision-making
and
predict
treatment
outcome.
In
particular,
rise
democratization
high-throughput
molecular
profiling
technologies
bolstered
"biomarker
signatures"
that
maximize
prediction
performance.
Such
an
approach
was
largely
employed
from
diverse
OMICs
data
(i.e.,
genomics,
transcriptomics,
proteomics,
metabolomics)
but
not
epitranscriptomics,
encompasses
more
than
100
biochemical
modifications
driving
post-transcriptional
fate
RNA:
stability,
splicing,
storage,
translation.
We
others
have
studied
chemical
marks
isolation
associated
them
with
evolution,
adaptation,
as
well
response
conventional
therapy.
this
study,
we
designed
a
unique
pipeline
combining
multiplex
analysis
epitranscriptomic
landscape
by
high-performance
liquid
chromatography
coupled
tandem
mass
spectrometry
statistical
multivariate
machine
learning
approaches
order
identify
biomarker
signatures
precision
medicine
improve
disease
diagnosis.
applied
analyze
cohort
adult
diffuse
glioma
patients
demonstrate
existence
"epitranscriptomics-based
signature"
permits
grades
be
discriminated
predicted
unmet
accuracy.
This
study
demonstrates
epitranscriptomics
(co)evolves
along
progression
opens
new
prospects
field
omics
personalized
medicine.
Frontiers in Molecular Biosciences,
Год журнала:
2022,
Номер
9
Опубликована: Сен. 27, 2022
Fragile
X-Syndrome
(FXS)
represents
the
most
common
inherited
form
of
intellectual
disability
and
leading
monogenic
cause
Autism
Spectrum
Disorders.
In
cases,
this
disease
results
from
absence
expression
protein
FMRP
encoded
by
FMR1
gene
(Fragile
X
messenger
ribonucleoprotein
1).
is
mainly
defined
as
a
cytoplasmic
RNA-binding
regulating
local
translation
thousands
target
mRNAs.
Interestingly,
also
able
to
shuttle
between
nucleus
cytoplasm.
However,
date,
its
roles
in
mammalian
neurons
are
just
emerging.
To
broaden
our
insight
into
contribution
nuclear
neuronal
physiology,
we
identified
here
interactome
combining
subcellular
fractionation
rat
forebrains
with
pull-
down
affinity
purification
mass
spectrometry
analysis.
By
approach,
listed
55
candidate
partners.
This
includes
known
FMRP-binding
proteins
Adar
or
Rbm14
well
several
novel
candidates,
notably
Ddx41,
Poldip3,
Hnrnpa3
that
further
validated
target-specific
approaches.
Through
factors
involved
different
steps
mRNA
biogenesis,
transcription,
splicing,
editing
export,
revealing
potential
central
regulatory
function
biogenesis
Therefore,
work
considerably
enlarges
interaction
network
lays
basis
for
exciting
future
mechanistic
studies
deepening
physiology
etiology
FXS.
Frontiers in Genetics,
Год журнала:
2022,
Номер
13
Опубликована: Окт. 6, 2022
Background:
5-methylcytosine
(m5C)
RNA
methylation
plays
a
significant
role
in
several
human
diseases.
However,
the
functional
of
m5C
type
2
diabetes
(T2D)
remains
unclear.
Methods:
The
merged
gene
expression
profiles
from
two
Gene
Expression
Omnibus
(GEO)
datasets
were
used
to
identify
m5C-related
genes
and
T2D-related
differentially
expressed
(DEGs).
Least-absolute
shrinkage
selection
operator
(LASSO)
regression
analysis
was
performed
optimal
predictors
T2D.
After
LASSO
regression,
we
constructed
diagnostic
model
validated
its
accuracy.
ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
analyses
conducted
confirm
biological
functions
DEGs.
Set
Enrichment
Analysis
(GSEA)
determine
enrichment
molecular
subtypes.
Weighted
co-expression
network
(WGCNA)
select
module
that
correlated
with
most
pyroptosis-related
genes.
Protein-protein
interaction
(PPI)
established
using
STRING
database,
hub
identified
Cytoscape
software.
competitive
endogenous
(ceRNA)
obtained.
CIBERSORT
algorithm
applied
analyze
interactions
between
immune
infiltration.
Results:
significantly
T2D
showed
ZBTB4
could
be
predictive
for
GO,
KEGG,
GSEA
indicated
enriched
modules
pathways
closely
related
metabolism-related
processes
cell
death.
top
five
as
PPI
network.
In
addition,
ceRNA
Moreover,
levels
abundance
various
cells.
Conclusion:
Our
findings
may
provide
insights
into
mechanisms
underlying
based
on
pathophysiology
suggest
potential
biomarkers
therapeutic
targets
Heliyon,
Год журнала:
2024,
Номер
10(21), С. e39036 - e39036
Опубликована: Окт. 5, 2024
Emerging
evidence
underscores
the
critical
role
of
impaired
mRNA
translation
in
various
neurobiological
conditions.
Ribosomal
RNA
(rRNA),
essential
for
protein
synthesis,
undergoes
crucial
post-transcriptional
modifications
such
as
2'-O-ribose
methylation,
pseudouridylation,
and
base
modifications.
These
modifications,
particularly
methylation
is
vital
stabilizing
rRNA
structures
optimizing
efficiency
by
regulating
integrity
its
interactions
with
proteins.
Concentrated
key
regions
like
decoding
sites
peptidyl
transferase
center,
dysregulation
these
can
disrupt
ribosomal
function,
contributing
to
pathogenesis
diverse
neurological
conditions,
including
mental
health
disorders,
developmental
abnormalities,
neurodegenerative
diseases.
Mechanistically,
involves
between
small
nucleolar
RNAs
(snoRNAs),
snoRNPs,
fibrillarin,
forming
a
complex
regulatory
network
maintaining
function.
Recent
research
highlights
association
defective
ribosome
biogenesis
spectrum
CNS
emphasizing
importance
understanding
mechanisms
disease
pathology.
This
review
focuses
on
pivotal
shaping
function
potential
implications
unraveling
pathophysiology
disorders.
Insights
gained
from
studying
could
pave
way
new
therapeutic
strategies
targeting
dysfunction
associated
neuropathological
advancing
precision
medicine
interventions.
Genes,
Год журнала:
2024,
Номер
15(12), С. 1599 - 1599
Опубликована: Дек. 14, 2024
Background/Objectives:
Aging
is
a
natural
physiological
process
involving
biological
and
genetic
pathways.
Growing
evidence
suggests
that
alterations
in
the
epigenome
during
aging
result
transcriptional
changes,
which
play
significant
role
onset
of
age-related
diseases,
including
cancer,
cardiovascular
disease,
diabetes,
neurodegenerative
disorders.
For
this
reason,
epigenetic
diseases
have
been
reviewed,
major
extrinsic
factors
influencing
these
identified.
In
addition,
gut
microbiome
its
metabolites
as
modifiers
has
addressed.
Results:
Long-term
exposure
to
such
air
pollution,
diet,
drug
use,
environmental
chemicals,
microbial
infections,
physical
activity,
radiation,
stress
provoke
changes
host
through
several
endocrine
immune
pathways,
potentially
accelerating
process.
Diverse
studies
reported
plays
critical
regulating
brain
cell
functions
DNA
methylation
histone
modifications.
The
interaction
between
genes
serves
source
adaptive
variation,
contributing
phenotypic
plasticity.
However,
molecular
mechanisms
signaling
pathways
driving
are
still
not
fully
understood.
Conclusions:
Extrinsic
potential
inducers
alterations,
may
important
implications
for
longevity.
an
effector
gene
expression
modifications,
while
bidirectional
interactions
with
underexplored
roles
non-bacterial
microorganisms
fungi
viruses
highlight
need
further
research.
