ZmG6PDH1 in glucose-6-phosphate dehydrogenase family enhances cold stress tolerance in maize

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Март 9, 2023

Glucose-6-phosphate dehydrogenase (G6PDH) is a key enzyme in the pentose phosphate pathway responsible for generation of nicotinamide adenine dinucleotide (NADPH), thereby playing central role facilitating cellular responses to stress and maintaining redox homeostasis. This study aimed characterize five G6PDH gene family members maize. The classification these ZmG6PDHs into plastidic cytosolic isoforms was enabled by phylogenetic transit peptide predictive analyses confirmed subcellular localization imaging using maize mesophyll protoplasts. These ZmG6PDH genes exhibited distinctive expression patterns across tissues developmental stages. Exposure stressors, including cold, osmotic stress, salinity, alkaline conditions, also significantly affected activity ZmG6PDHs, with particularly high isoform (ZmG6PDH1) response cold closely correlated enzymatic activity, suggesting that it may play shaping conditions. CRISPR/Cas9-mediated knockout ZmG6PDH1 on B73 background led enhanced sensitivity. Significant changes status NADPH, ascorbic acid (ASA), glutathione (GSH) pools were observed after exposure zmg6pdh1 mutants this disrupted balance contributing increased production reactive oxygen species resultant damage death. Overall, results highlight importance supporting resistance at least part producing NADPH can be used ASA-GSH cycle mitigate cold-induced oxidative damage.

Язык: Английский

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The Emerging Roles of the Metabolic Regulator G6PD in Human Cancers

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(24), С. 17238 - 17238

Опубликована: Дек. 7, 2023

Metabolic reprogramming, especially reprogrammed glucose metabolism, is a well-known cancer hallmark related to various characteristics of tumor cells, including proliferation, survival, metastasis, and drug resistance. Glucose-6-phosphate dehydrogenase (G6PD) the first rate-limiting enzyme pentose phosphate pathway (PPP), branch glycolysis, that converts glucose-6-phosphate (G6P) into 6-phosphogluconolactone (6PGL). Furthermore, PPP produces ribose-5-phosphate (R5P), which provides sugar-phosphate backbones for nucleotide synthesis as well nicotinamide adenine dinucleotide (NADPH), an important cellular reductant. Several studies have shown enhanced G6PD expression flux in their correlation with progression through regulation, reprogramming sustaining proliferative signaling, resisting cell death, activating invasion metastasis. Inhibiting could suppress promote reverse chemoresistance, inhibit suggesting potential target anti-tumor therapeutic strategies. Indeed, while challenges-including side effects-still remain, small-molecule inhibitors showing effect either when used alone or combination other drugs been developed. This review overview structural significance G6PD, its role regulation development progression, strategies explored relation G6PD-targeted therapy.

Язык: Английский

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Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality

Theriogenology, Год журнала: 2024, Номер 223, С. 36 - 46

Опубликована: Апрель 22, 2024

In vitro embryo production (IVP) is of great importance to the porcine industry, as well for basic research and biomedical applications. Despite large efforts made in laboratories worldwide address suboptimal culture conditions, IVP remains inefficient. Nobiletin (Nob, 5,6,7,8,3´,4´ hexamethoxyflavone) supplementation (IVC) medium, enhances development various species. However, its impact on quality developmental capacity vitro-produced pig embryos yet be established. This study evaluated effects different concentrations (2.5 5 μM) Nob during early competence, mitochondrial activity, lipid content, intracellular Reactive Oxygen Species (ROS) Glutathione (GSH) Total Cell Number (TCN) per blastocyst, expression genes related development, oxidative stress. Embryos cultured medium without supplemented with 0.01% dimethyl sulfoxide (DMSO-vehicle Nob) constituted Control DMSO groups, respectively. Embryo rates were Days 2, 6 7 IVC. Additionally, a representative group was selected assess lipid, ROS GSH content (on 2 IVC), TCN assessment gene analyses Day IVC). No significant differences observed any parameters contrast, under presence 2.5 showed higher addition, increased decreased levels compared other groups. Both Furthermore, upregulated Superoxide dismutase type 1 (SOD1) Glucose-6-phosphate dehydrogenase (G6PDH) genes, which could help counteract stress conclusion, addition first 48 h IVC enhanced their quality, including upregulation relevant that potentially improved overall efficiency system.

Язык: Английский

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Physiological Functions of Nicotinamide Coenzyme II

Опубликована: Янв. 1, 2025

Язык: Английский

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Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 26, 2025

Abstract Selective inheritance of sub-cellular components has emerged as a mechanism guiding stem cell fate after asymmetric divisions. Peroxisomes play crucial role in multiple metabolic processes such fatty acid metabolism and reactive oxygen species detoxification, but the apportioning peroxisomes during division remains understudied. Here, we develop mouse model labeling technique to follow dynamics distinct peroxisome age-classes, find that old are inherited by daughter retaining full potency mammary epidermal Old carry Glucose-6-phosphate-dehydrogenase, whose specific location on peroxisomal membrane promotes function facilitating ether lipid synthesis. Our study demonstrates age-selective vivo, unveils how functional heterogeneity is utilized asymmetrically dividing cells metabolically divert two cells.

Язык: Английский

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Metabolic plasticity sustains the robustness of Caenorhabditis elegans embryogenesis

EMBO Reports, Год журнала: 2023, Номер 24(12)

Опубликована: Окт. 27, 2023

Язык: Английский

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Genome-wide single nucleotide polymorphism (SNP) data reveal potential candidate genes for litter traits in a Yorkshire pig population

Archives animal breeding/Archiv für Tierzucht, Год журнала: 2023, Номер 66(4), С. 357 - 368

Опубликована: Ноя. 23, 2023

Abstract. The litter trait is one of the most important economic traits, and increasing size great value in pig industry. However, molecular mechanisms underlying traits remain elusive. To identify markers candidate genes for a genome-wide association study (GWAS) selection signature analysis were conducted Yorkshire population. A total 518 producing sows genotyped with Illumina Porcine SNP 50 BeadChip, 1969 farrowing records number born (TNB), alive (NBA), piglets dead (PBD), weight (LWB) collected. Then, GWAS was performed four using repeatability model. Based on estimated breeding values (EBVs) TNB, 15 high- low-prolificacy individuals selected from to implement analysis. Subsequently, signatures affecting detected by two methods including fixation index (FST) θπ. Combining results analysis, 20 promising (NKAIN2, IGF1R, KISS1R, TYRO3, SPINT1, ADGRF5, APC2, PTBP1, CLCN3, CBR4, HPF1, FAM174A, SCP2, CLIC1, ZFYVE9, SPATA33, KIF5C, EPC2, GABRA2, GABRA4) identified. These findings provide novel insights into genetic basis will be helpful improving reproductive performances breeding.

Язык: Английский

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Effects of astaxanthin in diet on antioxidant capacity and molting-related genes expression of larvae giant freshwater prawn (Macrobrachium rosenbergii)

Aquaculture Reports, Год журнала: 2024, Номер 35, С. 101956 - 101956

Опубликована: Фев. 3, 2024

This experiment was carried out to determine the effects of astaxanthin on reactive oxide species (ROS), antioxidant capacity as well molting-related genes expression in larvae giant freshwater prawn, Macrobrachium rosenbergii. A total 90,000 were assigned five treatments with six replicates each. Astaxanthin mixed steamed eggs graded effective content 0 (control, A0), 40 (A40), 80 (A80), 160 (A160) and 320 (A320) mg kg−1 fed for 14 days. The results showed that ROS significantly lower than control (P < 0.05). T-AOC, T-SOD GR activities under generally a downward trend decreased No significant difference observed towards CAT among A40 A80, but values markedly greater A0, A160, A320 Larvae A80 exhibited stronger MDA other GSH-Px activity highest molt-inhibiting hormone (MIH) enhanced elevated Ecdysone receptor (EcR) recorded only A160 Chitinase (CHIT) downregulated except A40. Heat shock protein (HSP21) chitooligosaccharidolytic beta-N-acetylglucosaminidase-like increased dramatically For actin 6 gene, higher happened compared control, others presented drop very low level study provides theoretical basis mg/kg excessive level, might partial indexes influenced molting M.

Язык: Английский

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Delineation of the role of G6PD in Alzheimer’s disease and potential enhancement through microfluidic and nanoparticle approaches

Ageing Research Reviews, Год журнала: 2024, Номер 99, С. 102394 - 102394

Опубликована: Июнь 29, 2024

Язык: Английский

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An Overall View of the Functional and Structural Characterization of Glucose-6-Phosphate Dehydrogenase Variants in the Mexican Population

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(16), С. 12691 - 12691

Опубликована: Авг. 11, 2023

Glucose-6-phosphate dehydrogenase (G6PD) deficiency, affecting an estimated 500 million people worldwide, is a genetic disorder that causes human enzymopathies. Biochemical and studies have identified several variants produce different ranges of phenotypes; thus, depending on its severity, this enzymopathy classified from the mildest (Class IV) to most severe I). Therefore, understanding correlation between mutation sites G6PD resulting phenotype greatly enhances current knowledge enzymopathies’ phenotypic genotypic heterogeneity, which will assist both clinical diagnoses personalized treatments for patients with deficiency. In review, we analyzed compared structural functional data 21 characterized found in Mexican population previously characterized. order contribute regarding function structure associated review aimed determine molecular basis identify how these mutations could impact structure, stability, enzyme relation manifestations disease.

Язык: Английский

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