Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Март 28, 2025
Язык: Английский
Hereditas, Год журнала: 2025, Номер 162(1)
Опубликована: Янв. 9, 2025
Abstract Background Cervical cancer (CC) is a prevalent gynecological malignancy, contributing to substantial number of fatalities among women. MicroRNAs (miRNAs) have emerged as promising biomarkers with significant potential for the early detection and prognosis CC. Objective This study aimed explore clinical significance biological role miR-615-5p in CC, goal identifying novel this disease. Materials methods The levels TMIGD2 mRNA tissue samples cells were quantified through quantitative reverse transcription real-time PCR, followed by statistical analyses investigate correlation between data. effects on proliferation metastasis CC evaluated using Cell Counting Kit-8 Transwell assays. mechanism was elucidated bioinformatics Dual-luciferase reporter assay. Western blotting employed measure protein TMIGD2. Results In downregulation related poor an independent prognostic factor. reduced cells. overexpression restrained Furthermore, identified target gene regulated miR-615-5p, its expression notably elevated influence behaviors mediated modulation Conclusions Downregulation indicator exerted tumor-suppressive inhibiting cell growth regulation
Язык: Английский
Процитировано
0
Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 291, С. 117800 - 117800
Опубликована: Фев. 1, 2025
Long-term exposure to hexavalent chromium [Cr(VI)] has been linked lung cancer, and cyclooxygenase-2 (COX-2) is a well-known inflammatory factor. However, the role mechanism of COX-2 in Cr(VI)-induced carcinogenesis are not clear yet. To address this question, we employed mouse model exposed Cr(VI) through intranasal instillation particulate zinc chromate (ZnCrO4) for 12 weeks. Metabolomics RNA-seq assays revealed enhanced activity arachidonic acid (AA)/eicosanoid metabolism pathway tissues from mice Cr(VI). COX-2, key enzyme AA/eicosanoid pathway, was significantly upregulated Cr(VI)-exposed tissues, as well transformed (Cr-T) cells compared parental BEAS-2B (B2B) cells. We then multidisciplinary vitro vivo functional characterize cancer. The results indicated that functioned an oncogene promote malignant transformation B2B enhance proliferation, migration, tumor growth, angiogenesis Cr-T Nuclear factor E2-related factor-2 (Nrf2) identified transcription COX-2. Nrf2 response contributed cancers, part by upregulating expression. Moreover, microRNA-379 (miR-379) found target inhibit its expression posttranscriptionally. MiR-379 downregulated cells, ectopic miR-379 reduced cell viability with partial reversal upon restoration. In summary, our study oncogenic two novel regulatory mechanisms overexpression carcinogenesis.
Язык: Английский
Процитировано
0
Discover Oncology, Год журнала: 2025, Номер 16(1)
Опубликована: Март 10, 2025
Ovarian cancer is associated with the highest mortality rate among all malignant gynecological tumors. PolyADP-ribose polymerase (PARP) inhibitor maintenance therapy standard treatment strategy for this type of cancer, and olaparib a widely used oral PARP tumors BRCA mutations. The present study aimed to investigate effects in non-BRCA-mutated ovarian potential mechanisms involved. antitumor effect cisplatin alone or combination was analyzed an subcutaneous transplantation tumor model nude mice. Furthermore, differences microRNA (miRNA) expression levels were using miRNA arrays. In addition, miR-125a-3p on proliferation (A2780 OVCAR-3) cells detected A Cell Counting Kit-8 changes cell cycle flow cytometry. SPiDER-βGal detect cellular senescence, DNA damage repair proteins western blot analysis. results revealed that plus significantly reduced volume mice subjected transplantation, increased combination. overexpression could inhibit migration, invasion induces arrest. On whole, demonstrates senescence cells, which enhances therapeutic sensitivity.
Язык: Английский
Процитировано
0
Polymers, Год журнала: 2025, Номер 17(7), С. 915 - 915
Опубликована: Март 28, 2025
The use of miRNA in therapeutics has, since its discovery 1993, attracted tremendous attention, and research this area has progressed rapidly. Since the advent RNA interference (RNAi), much about nucleic acid siRNA been elucidated. At same time, no miRNA-based drugs have passed phase II clinical trials. A significant obstacle to drug development is ease degradation relatively short half-life vivo miRNA. Hydrogels are networks cross-linked polymer chains with ability 'swell'. They shown remarkable capabilities that improve properties other researched carriers. In combination modification strategies inorganic carriers, hydrogel systems show promise for sustained delivery novel drugs. Although reported recently, focus predominantly on their wound-healing properties, a dearth information carrier abilities. This paper focuses more latest advancements developing hydrogels as system, emphasizing review will cover methods fabrication, efforts release, biomedical applications, future prospects.
Язык: Английский
Процитировано
0
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Март 28, 2025
Язык: Английский
Процитировано
0