Serum direct SMOS-qPCR: a fast approach for miRNAs detection

Analytical Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

A novel serum direct SMOS-qPCR method enables amplification of multiple miRNAs from samples without requiring separate extraction and purification steps.

Язык: Английский

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Expression Analysis of Circulating miR-21, miR-34a and miR-122 and Redox Status Markers in Metabolic Dysfunction-Associated Steatotic Liver Disease Patients with and Without Type 2 Diabetes

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2392 - 2392

Опубликована: Март 7, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD), a hepatic form of metabolic syndrome, often co-occurs with type 2 diabetes (T2D) and now affects approximately 30% the global population. MASLD encompasses conditions from simple steatosis to steatohepatitis, oxidative stress (OS) driving progression through inflammation. This study analyzes expression levels circulating miRNAs redox status markers in patients without T2D, exploring their potential as biomarkers. The expressions miR-21, miR-34a, miR-122 were analyzed platelet-poor plasma 147 participants, divided into three groups: + T2D (48), (50), control group (49). Total oxidant (TOS), total antioxidant (TAS), ischemia-modified albumin (IMA), superoxide anion radical (O2•−) measured serum plasma. Logistic regression showed that TOS, TAS, O2•−, IMA positive predictors MASLD, while miR-21 TAS negative MASLD. Although did not show significant association either condition, combination miR-34a other such lipid enzymes, new predictor was identified. Circulating markers, enzymes

Язык: Английский

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A PEG-based strategy to improve detection of clinical microRNA 155 by bio-Field Effect Transistor in high ionic strength environment

Talanta, Год журнала: 2025, Номер 292, С. 127881 - 127881

Опубликована: Март 11, 2025

microRNAs are small oligonucleotides involved in post-transcriptional gene regulation whose alteration is found several diseases, including cancer, and therefore their detection crucial for diagnosis, prognosis, treatment purposes. Field-Effect Transistor-based biosensors (bioFETs) represent a promising technology the clinical of microRNAs. However, one main challenges associated with this Debye screening, becoming significant at high ionic strengths required effective hybridization. We aimed detecting oncogenic microRNA-155 by using bioFET system as capture element complementary RNA probe (antimiR-155) combined introduction PEG molecules (20 kDa, PEG20), an strength 300 mM. optimized co-immobilization ratio between antimiR-155 PEG20 assessed its impact on interactions oligonucleotides. The kinetics can be well described Langmuir-Freundlich isotherm affinity constant within range typical nucleic acid interactions. significantly enhanced sensitivity miR-155 reaching level less than 200 pM, together excellent discrimination against other clinically relevant Our findings demonstrate that incorporation constitutes strategy to mitigate screening effects facilitates bioFET-based applications physiological strengths.

Язык: Английский

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MicroRNAs as Epigenetic Biomarkers of Pathogenetic Mechanisms of the Metabolic Syndrome Induced by Antiseizure Medications: Systematic Review

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(7), С. 2432 - 2432

Опубликована: Апрель 2, 2025

Antiseizure medication (ASM) induced metabolic syndrome (AIMetS) is a common adverse drug reaction (ADR) of pharmacotherapy for epilepsy and psychiatric disorders. However, the sensitivity specificity blood biomarkers may be insufficient due to influence combined pathology, concomitant diseases, peculiarities metabolism ASMs in patients with epilepsy. Methods: The presented results experimental clinical studies microRNAs (miRs) as epigenetic MetS AIMetS, which were entered into different databases, analyzed last decade (2014-2024). Results: A systematic review demonstrated that miRs can act promising key AIMetS domains. variable role various their paralogs pathogenesis AIMetS. Therefore, part this study, an signature was proposed allows us assess risk developing severity low risk, medium high risk. Conclusions: mechanisms development are actual problem epileptology, still far from being resolved. panels (signatures) widespread ADR help increase safety circulating it necessary conduct "bridge" order replicate preclinical real practice.

Язык: Английский

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Perspectives in MicroRNA Therapeutics for Cystic Fibrosis

Non-Coding RNA, Год журнала: 2025, Номер 11(1), С. 3 - 3

Опубликована: Янв. 12, 2025

The discovery of the involvement microRNAs (miRNAs) in cystic fibrosis (CF) has generated increasing interest past years, due to their possible employment as a novel class drugs be studied pre-clinical settings therapeutic protocols for fibrosis. In this narrative review article, consider and comparatively evaluate published laboratory information development miRNA-based We miRNAs involved upregulation CFTR, inhibition inflammation and, finally, exhibiting antibacterial activity. suggest that antago-miRNAs ago-miRNAs (miRNA mimics) can proposed validation settings.

Язык: Английский

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Computer analysis shows differences between mitochondrial miRNAs and other miRNAs

Vavilov Journal of Genetics and Breeding, Год журнала: 2025, Номер 28(8), С. 834 - 842

Опубликована: Янв. 25, 2025

A subclass of miRNAs with as yet unknown specific functions is mitomiRs – mitochondrial that are mainly derived from nuclear DNA and imported into mitochondria; moreover, changes in the expression levels associated some diseases. To identify most pronounced characteristics distinguish them other miRNAs, we classified mitomiR sequences using Random Forest algorithm. The analysis revealed, for first time, a significant difference between microRNAs by following criteria (in descending order importance classification): evolutionarily older (have lower phylostratigraphic age index, PAI); have more targets disease associations, including ones (twosided Fisher’s exact test, average p -values 1.82×10 –89 /1.13×10 –96 all mRNA/diseases 6.01×10 –22 /1.09×10 –9 mitochondria, respectively); class “circulating” (average pvalue 1.20×10 –56 ). identified differences may help uncover mode miRNA delivery indicate evolutionary conservation regulation function metabolism, generally show not randomly encountered miRNAs. Information on 1,312 experimentally validated three organisms ( Homo sapiens, Mus musculus Rattus norvegicus ) collected mitomiRdb database https://mitomiRdb.org

Язык: Английский

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Circulating MicroRNAs in Patients with Vulvar Squamous Cell Carcinoma and Its Precursors

Non-Coding RNA, Год журнала: 2025, Номер 11(1), С. 13 - 13

Опубликована: Фев. 7, 2025

Objectives: Vulvar squamous cell carcinoma (VSCC) is a rare gynecologic malignancy, with most cases arising from differentiated vulvar intraepithelial neoplasia (dVIN). Approximately one-third of VSCC originate high-grade lesions (HSILs), which are associated persistent infection by varieties high-risk human papillomavirus (hrHPV). This study aimed to quantify the circulating microRNAs (miRNAs) in plasma patients premalignant conditions (dVIN and HSILs) using TaqMan Low-Density Arrays. Methods: Plasma samples were collected 40 patients, including those treated for HSILs, dVIN, VSCC. Quantitative real-time PCR (qRT-PCR) identified miRNAs differentially expressed compared precancerous lesions. Results: A total 31 (DEMs) found be significantly upregulated cases. None analyzed able distinguish based on hrHPV tumor status. Conclusions: provides strong evidence that distinct set can differentiate between Thus, these DEMs have potential diagnostic prognostic value. "Predisposing" could developed as biomarkers aid assessment lesions, helping exclude or confirm progression toward cancer.

Язык: Английский

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Persistent organic pollutants and plasma MicroRNAs: A community-based profiling analysis

Environment International, Год журнала: 2025, Номер 197, С. 109328 - 109328

Опубликована: Фев. 19, 2025

Язык: Английский

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MicroRNA profiling in umbilical cord plasma: links to maternal metabolism and neonatal metabolic and inflammatory traits

The Journal of Physiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

MicroRNAs (miRNAs) are regulators of mRNA translation and play crucial roles in various physiological pathological processes. In this study, we profiled miRNAs umbilical cord plasma (UCP) to explore the association neonatal circulating with maternal metabolic parameters anthropometric, inflammatory characteristics healthy pregnancies. Data UCP samples were collected from 16 pregnancies, equally divided between normal-weight overweight mothers male female newborns. Using next-generation sequencing, identified quantified UCP, alongside analysis parameters. Our results revealed that majority sensitive characteristics, particularly body mass index, gestational weight gain, placental weight, leptin, C-reactive protein insulin levels. Notably, a strong placenta-derived chromosome 19 microRNA cluster (C19MC) weight. Likewise, pregnancy-specific 14 (C14MC) was associated index leptin. study highlights sensitivity conditions, demonstrates their traits, underscores potential role blood fetal metabolism development. KEY POINTS: regulatory RNA molecules modulate expression. They present all fluids affected by changes. Pregnancy is state change mother, affects We found composition metabolism. Pregnancy-specific groups showed particular patterns, encoded region specific insulin. represent separate dimension through which can influence

Язык: Английский

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Primer Exchange Reaction Coupled with DNA-Templated Silver Nanoclusters for Label-Free and Sensitive Detection of MicroRNA

Applied Biochemistry and Biotechnology, Год журнала: 2023, Номер 195(10), С. 6334 - 6344

Опубликована: Март 2, 2023

Язык: Английский

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