Animals,
Год журнала:
2023,
Номер
13(13), С. 2195 - 2195
Опубликована: Июль 4, 2023
The
aim
of
the
study
was
to
investigate
potential
cell-based
regenerative
therapy
for
elbow
joints
affected
by
osteoarthritis.
Interest
focused
on
two
intra-articular
applications
amnion-derived
mesenchymal
stem
cells
(A-MSCs)
a
group
different
breeds
dogs
with
osteoarthritis
(13
joints).
Two
injections
were
performed
14
days
apart.
We
evaluated
synovial
fluid
biomarkers,
such
as
IFN-γ,
IL-6,
IL-15,
IL-10,
MCP-1,
TNF-α,
and
GM-CSF,
multiplex
fluorescent
micro-bead
immunoassay
in
treated
elbows
(n
=
13)
(day
0,
day
14,
28)
control
9).
Kinematic
gait
analysis
determined
joint
range
motion
(ROM)
before
after
each
A-MSCs
application.
28.
pointed
out
improvement
average
from
0
(38.45
±
5.74°),
(41.7
6.04°),
28
(44.78
4.69°)
statistical
significance
(p
<
0.05)
nine
elbows.
Correlation
analyses
proved
associations
between
ROM
GM-CSF
concentrations
28).
TNF-
α
showed
negative
correlation
at
28,
while
IL-10
demonstrated
positive
ROM.
As
consequence
A-MSC
application
joint,
we
detected
statistically
significant
decrease
concentration
levels
TNF-α
increase
IL-10.
Statistical
well
group.
IL-6
Frontiers in Cell and Developmental Biology,
Год журнала:
2023,
Номер
11
Опубликована: Июнь 23, 2023
Bone
healing
is
associated
with
many
orthopedic
conditions,
including
fractures
and
osteonecrosis,
arthritis,
metabolic
bone
disease,
tumors
periprosthetic
particle-associated
osteolysis.
How
to
effectively
promote
has
become
a
keen
topic
for
researchers.
The
role
of
macrophages
marrow
mesenchymal
stem
cells
(BMSCs)
in
gradually
come
light
the
development
concept
osteoimmunity.
Their
interaction
regulates
balance
between
inflammation
regeneration,
when
inflammatory
response
over-excited,
attenuated,
or
disturbed,
it
results
failure
healing.
Therefore,
an
in-depth
understanding
function
regeneration
relationship
two
could
provide
new
directions
This
paper
reviews
mechanism
significance
their
interaction.
Several
therapeutic
ideas
regulating
by
targeting
crosstalk
are
also
discussed.
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
173, С. 116458 - 116458
Опубликована: Март 19, 2024
Osteoarthritis
(OA)
is
a
multifaceted
disease
characterized
by
imbalances
in
extracellular
matrix
metabolism,
chondrocyte
and
synoviocyte
senescence,
as
well
inflammatory
responses
mediated
macrophages.
Although
there
have
been
notable
advancements
pharmacological
surgical
interventions,
achieving
complete
remission
of
OA
remains
formidable
challenge,
oftentimes
accompanied
significant
side
effects.
Mesenchymal
stem
cells
(MSCs)
emerged
promising
avenue
for
treatment,
given
their
ability
to
differentiate
into
chondrocytes
facilitate
cartilage
repair,
thereby
mitigating
the
impact
an
microenvironment
induced
This
comprehensive
review
aims
provide
concise
overview
diverse
roles
played
MSCs
treatment
OA,
while
elucidating
underlying
mechanisms
behind
these
contributions.
Specifically,
include:
(a)
Promotion
regeneration;
(b)
Inhibition
degradation;
(c)
Attenuating
macrophage-induced
microenvironment;
(d)
Alleviation
pain.
Understanding
paramount
developing
novel
therapeutic
strategies.
By
harnessing
regenerative
potential
immunomodulatory
properties
MSCs,
it
may
be
possible
devise
more
effective
safer
approaches
managing
OA.
Further
research
clinical
studies
are
warranted
optimize
utilization
realize
full
field
therapeutics.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(15), С. 12282 - 12282
Опубликована: Июль 31, 2023
Osteoarthritis
(OA)
is
characterized
by
degeneration
of
the
joint
cartilage,
inflammation,
and
a
change
in
chondrocyte
phenotype.
Inflammation
also
promotes
cell
hypertrophy
human
articular
chondrocytes
(HC-a)
activating
NF-κB
pathway.
Chondrocyte
inflammation
promote
extracellular
matrix
degradation
(ECM).
Chondrocytes
depend
on
Smad
signaling
to
control
regulate
as
well
maintain
ECM.
The
involvement
these
two
pathways
crucial
for
preserving
homeostasis
cartilage.
In
recent
years,
Polynucleotides
Highly
Purified
Technology
(PN-HPT)
has
emerged
promising
area
research
treatment
OA.
PN-HPT
involves
use
polynucleotide-based
agents
with
controlled
natural
origins
high
purification
levels.
this
study,
we
focused
evaluating
efficacy
specific
polynucleotide
sodium
agent,
known
CONJURAN,
which
derived
from
fish
sperm.
(PN),
are
physiologically
present
function
water-soluble
nucleic
acids
gel-like
property,
have
been
used
treat
patients
However,
mechanisms
underlying
effect
remain
unclear.
Therefore,
investigated
PN
an
OA
model
HC-a
cells
were
stimulated
interleukin-1β
(IL-1β)
or
without
treatment.
CCK-8
assay
was
assess
cytotoxic
effects
PN.
Furthermore,
enzyme-linked
immunosorbent
utilized
detect
MMP13
levels,
nitric
oxide
determine
inflammation.
anti-inflammatory
related
using
quantitative
PCR,
Western
blot
analysis,
immunofluorescence
examine
analyze
relative
markers.
inhibited
IL-1β
induced
destruction
genes
proteins
downregulating
expression
MMP3,
MMP13,
iNOS,
COX-2
while
increasing
aggrecan
(ACAN)
collagen
II
(COL2A1).
This
study
demonstrates,
first
time,
that
exerted
partially
inhibiting
pathway
Smad2/3
Based
our
findings,
can
potentially
serve
ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(5), С. 3355 - 3377
Опубликована: Апрель 2, 2024
An
imbalance
between
M1
and
M2
macrophage
polarization
is
critical
in
osteoarthritis
(OA)
development.
We
investigated
the
effect
of
macrophage-derived
extracellular
vesicles
(M2-EVs)
to
reprogramme
macrophages
from
phenotype
for
OA
treatment.
mouse
models
were
treated
with
M2-EVs.
Proteomic
analysis
was
performed
evaluate
vitro.
The
as
follows:
destabilization
medial
meniscus
(DMM)
surgery-induced
collagenase-induced
(CIOA).
Hyaluronic
acid
(HA)
used
deliver
M2-EVs
decreased
accumulation,
repolarized
phenotype,
mitigated
synovitis,
reduced
cartilage
degradation,
alleviated
subchondral
bone
damage,
improved
gait
abnormalities
CIOA
DMM
models.
Moreover,
HA
increased
retention
time
enhanced
efficiency
Furthermore,
proteomic
demonstrated
that
exhibited
a
reprogramming
ability
similar
IL-4,
pathways
might
be
NOD-like
receptor
(NLR),
TNF,
NF-κB,
Toll-like
(TLR)
signaling
pathways.
reprogrammed
which
resulted
beneficial
effects
on
attenuation
severity.
In
summary,
our
study
indicated
M2-EV-guided
promising
treatment
strategy
OA.
ACS Omega,
Год журнала:
2024,
Номер
9(19), С. 21467 - 21483
Опубликована: Май 1, 2024
Osteoarthritis
(OA)
is
a
chronic
musculoskeletal
disorder
characterized
by
cartilage
degeneration
and
synovial
inflammation.
Paracrine
interactions
between
chondrocytes
macrophages
play
an
essential
role
in
the
onset
progression
of
OA.
In
this
study,
replicating
inflammatory
response
during
OA
pathogenesis,
were
treated
with
interleukin-1β
(IL-1β),
lipopolysaccharide
interferon-γ.
addition,
coculture
system
was
developed
to
simulate
biological
situation
joint.
we
examined
impact
hyaluronic
acid
(HA)
viscosupplement,
particularly
Hyruan
Plus,
on
macrophages.
Notably,
viscosupplement
has
demonstrated
promising
outcomes
reducing
inflammation;
however,
underlying
mechanism
action
remains
elusive.
The
attenuated
inflammation,
showing
inhibitory
effect
nitric
oxide
production,
downregulating
proinflammatory
cytokines
such
as
matrix
metalloproteinases
(MMP13
MMP3),
upregulating
expression
levels
type
II
collagen
aggrecan
chondrocytes.
HA
also
reduced
level
IL-1β,
TNF-α,
IL-6
macrophages,
exerted
overall
protective
partially
suppressing
MAPK
pathway
p65/NF-κB
signaling
Therefore,
shows
potential
for
treating
arthritic
joints.
Naunyn-Schmiedeberg s Archives of Pharmacology,
Год журнала:
2024,
Номер
397(10), С. 8023 - 8041
Опубликована: Май 22, 2024
Diabetic
cardiomyopathy
(DCM)
is
a
serious
common
complication
of
diabetes.
Unfortunately,
there
no
satisfied
treatment
for
those
patients
and
more
studies
are
in
critical
need
to
cure
them.
Therefore,
we
aimed
carry
out
our
current
research
explore
the
role
two
novel
therapeutic
approaches:
one
biological
drug
block
inflammatory
signaling
IL
1beta
(IL1β)
axis,
namely,
anakinra;
other
provision
anti-inflammatory
regenerative
stem
cells.
Wistar
male
rats
were
allocated
into
four
groups:
control
group:
type
2
diabetes
mellitus
(DM)
induced
by
6-week
high-fat
diet
(HFD)
followed
single-dose
streptozotocin
(STZ)
35
mg/kg
i.p.,
then
into:
DM:
untreated;
DM
BM-MSCs:
received
single
dose
BM-MSCs
(1
×
10
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 7, 2025
Exosomes
(EXOs)
as
a
targeted
cell-free
therapy
could
offer
new
therapeutic
strategy
for
immune-mediated
inflammatory
diseases,
due
to
their
stability
and
ease
of
storage
handling.
This
study
focused
on
exosomes
derived
from
stem
cells
human
exfoliated
deciduous
teeth
(SHED-MSC-EXOs)
role
in
managing
the
balance
immunoregulatory
macromolecules
that
play
underlying
mechanisms
THP-1-derived
M0/M1
macrophage
cells.
Flow
cytometry
confirmed
expression
CD14,
CD68,
CD80,
CD86
markers
these
macrophages.
Following
morphological
survival
assessments,
culture
supernatants
SHED-MSCs
were
used
isolate
exosomes.
Once
verified,
Calcein
AM-labeled
EXOs
introduced
The
assessed
by
analyzing
surface
markers,
cytokine
production,
pro-
antioxidant
activity.
Macrophages
treated
with
exhibited
immunomodulatory
effects
akin
those
dexamethasone.
levels
anti-inflammatory
including
CD206,
Arg-1,
IL-10,
TGF-β,
TAC,
CAT,
SOD,
which
act
immunosuppressive
macromolecules,
elevated.
In
contrast,
there
was
reduction
pro-inflammatory
pro-oxidant
CD81,
IL-6R,
IL-12,
TNF-α,
MDA,
NO,
immunostimulatory
(P
<
0.05).
findings
suggest
SHED-MSC
can
skew
macrophages
M2
phenotype
inhibit
M1
polarization.
These
nanovesicles,
distinct
physical
properties
ability
penetrate
target
cells,
may
prove
beneficial
conditions
involving
depletion
macrophage-induced
potentially
aiding
inflammation
tissue
injury.
