Associations of urinary caffeine metabolites with sex hormones: comparison of three statistical models

Frontiers in Nutrition, Год журнала: 2025, Номер 11

Опубликована: Янв. 7, 2025

The association between urinary caffeine and metabolites with sex hormones remains unclear. This study used three statistical models to explore the associations its among adults. We selected participants aged ≥18 years in National Health Nutrition Examination Survey (NHANES) data 2013-2014 as our subjects. performed principal components analysis (PCA) investigate underlying correlation structure of metabolites. Then we these (PCs) independent variables conduct multiple linear regression (E2, TT, SHBG). also fitted weighted quantile sum (WQS) regression, Bayesian kernel machine (BKMR) methods further assess relationships. In PCA-multivariable PC2 negatively correlates E2: β = -0.01, p-value 0.049 (male population). WQS model, indices were associated SHBG TT both male (SHBG: index -0.11, p < 0.001; TT: -0.10, 0.001) female -0.04, groups. Besides, was significantly E2 females (p 0.05). BKMR despite no significant difference overall SHBG), there nonetheless a declining trend population group, groups observed downward trend. When considering results models, whole-body burden metabolites, especially metabolic pathway males. Considering advantages disadvantages recommend applying diverse interpreting their together.

Язык: Английский

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The Crucial Role of the Blood–Brain Barrier in Neurodegenerative Diseases: Mechanisms of Disruption and Therapeutic Implications

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(2), С. 386 - 386

Опубликована: Янв. 9, 2025

The blood-brain barrier (BBB) is a crucial structure that maintains brain homeostasis by regulating the entry of molecules and cells from bloodstream into central nervous system (CNS). Neurodegenerative diseases such as Alzheimer's Parkinson's disease, well ischemic stroke, compromise integrity BBB. This leads to increased permeability infiltration harmful substances, thereby accelerating neurodegeneration. In this review, we explore mechanisms underlying BBB disruption, including oxidative stress, neuroinflammation, vascular dysfunction, loss tight junction integrity, in patients with neurodegenerative diseases. We discuss how breakdown contributes neurotoxicity, abnormal accumulation pathological proteins, all which exacerbate neuronal damage facilitate disease progression. Furthermore, potential therapeutic strategies aimed at preserving or restoring function, anti-inflammatory treatments, antioxidant therapies, approaches enhance integrity. Given role neurodegeneration, maintaining its represents promising approach slow prevent progression

Язык: Английский

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Analyzing the Effect of Coffee Consumption on Visual Pathway Using Visual Evoked Potential (VEP) Signals and Machine Learning Algorithms

Communications in computer and information science, Год журнала: 2025, Номер unknown, С. 267 - 277

Опубликована: Янв. 1, 2025

Язык: Английский

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Caffeine Inhibits Tau Aggregation and Destabilizes the Fibril Associated with Chronic Traumatic Encephalopathy: A REST2 and Conventional MD Simulations Study

Journal of Chemical Information and Modeling, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Chronic traumatic encephalopathy (CTE) is a unique tauopathy mostly diagnosed in contact sports athletes, such as those active American football, boxing, soccer, etc. The hyperphosphorylated fibrillar aggregates composed of self-assembled tau protein are the pathological hallmark CTE, and inhibiting aggregation or disassociating has been considered promising avenue to prevent treat CTE. Caffeine (CA) well-known psychostimulant can be found coffee, tea, soft drinks. In vitro experiments revealed that CA could effectively inhibit wild-type disassemble preformed fibrils. However, atomic effect underlying molecular mechanisms remain largely elusive. this study, we performed multitude replica exchange with solute tempering 2 (REST2) conventional dynamics (CMD) simulations 43.8 μs total on models without CA, including third fourth microtubule-binding repeats (R3-R4) monomer CTE-related R3-R4 protofibril fibril. results prominently β-sheet formation disrupt structure protofibril, inducing adopt loosely packed extended conformations. H-bonding π-π stacking interactions drove binding monomer, while hydrophobic made an extra contribution protofibril. Strikingly, stably bind cavity which might occupy space entering cofactor. What more, destabilized fibril played role reversing liquid-to-solid phase transition (LSPT) tau. Our study systematically uncovered atomic-level aggregation, offers theoretical foundation for design drugs

Язык: Английский

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Exploring energy drink consumption among nursing students: A qualitative study

Teaching and learning in nursing, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Self-assembly and 3D Bioprinting of Neurospheres and Evaluation of Caffeine and Photobiomodulation Effects in an Alzheimer’s Disease In Vitro Model

Stem Cell Reviews and Reports, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Язык: Английский

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Safety Pharmacological Assessment of Oral Theophylline Administration on Respiratory Function in Sprague–Dawley Rats

Journal of Medicinal Food, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

In this study, we evaluated the safety pharmacological effects and potential adverse reactions of orally administered theophylline. Theophylline, a commonly used bronchodilator, is xanthine derivative that exerts stimulatory on central nervous system. Using an experimental rat model, explored impact theophylline respiratory parameters, including rate, tidal volume, minute volume. Thirty-two 6-week-old male Sprague-Dawley rats were randomly divided into control groups. The group received single oral dose 100 mg/kg Respiratory parameters measured at baseline (0 h) 0.5, 1, 2, 4, 6 h post administration using whole-body plethysmograph. Oral significantly increased rate volume all postdose time points compared with those in group, peak values observed 0.5 postadministration (P < .05). contrast, remained consistent across points, indicating enhances efficiency primarily by increasing frequency breaths rather than altering their depth. Our study demonstrated ability to enhance via breathing without affecting thus offering insights clinical significance managing chronic diseases.

Язык: Английский

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Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat

Antioxidants, Год журнала: 2023, Номер 12(2), С. 295 - 295

Опубликована: Янв. 28, 2023

High-risk preterm infants are affected by a higher incidence of cognitive developmental deficits due to the unavoidable risk factor oxygen toxicity. Caffeine is known have protective effect in preventing bronchopulmonary dysplasia associated with improved neurologic outcomes, although very early initiation therapy controversial. In this study, we used newborn rats an injury model test hypothesis that near-birth caffeine administration modulates neuronal maturation and differentiation hippocampus developing brain. For purpose, Wistar were exposed 21% or 80% on day birth for 3 5 days treated vehicle (10 mg/kg/48 h). Postnatal exposure resulted drastic reduction mediators radial glia, mitotic/postmitotic neurons, impaired cell-cycle regulation, predominantly persistent even after recovery room air until postnatal 15. Systemic significantly counteracted effects insult hippocampus. Interestingly, under normoxia, inhibited transcription maturing mature neurons. The modulated hyperoxia-induced decreased neurogenesis showed neuroprotective properties neonatal rat toxicity model.

Язык: Английский

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Characterization of tea (Camellia sinensis L.) flower extract and insights into its antifungal susceptibilities of Aspergillus flavus

BMC Complementary Medicine and Therapies, Год журнала: 2023, Номер 23(1)

Опубликована: Авг. 14, 2023

Abstract Background Tea ( Camellia sinensis L.) flowers will compete with tea leaves in nutrition and are abandoned as an undesirable by-product. In this study, the biological efficacy of was investigated. Further exploration its antifungal activity explained. Methods harvested from China were characterized term component, antioxidant ability, tyrosinase inhibition, ability. Chemical compounds analyzed by LC-MS. Disinfectant identified flowers, 2-ketobutyric acid exhibited against Aspergillus flavus CCTCC AF 2023038. The mechanism further investigated RNA-seq. Results Water-soluble flower extracts (TFEs) free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) well a high ferric-reducing However, no inhibition observed. test, 6.4 mg/mL TFE reached 71.5% rate electrical conductivity culture broth increased increasing concentration TFE, implying that it damaged fungal cell membrane TFE. Several disinfectants LC-MS, also confirmed to be capable inhibition. Propidium iodide (PI) staining indicated caused damage membrane. RNA-seq analysis revealed 3,808 differentially expressed genes (DEGs) found A. 2023038 treated acid, more than 1,000 DEGs involved integral intrinsic component affected. Moreover, downregulated aflatoxin biosynthesis decreased production. Conclusions Overall, excellent ability due abundant disinfectant compounds. As recognized food additive, is safe use industry can utilized basis for research development strong fungicides.

Язык: Английский

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Exploring metabolic dynamics during the fermentation of sea buckthorn beverage: comparative analysis of volatile aroma compounds and non-volatile metabolites using GC–MS and UHPLC–MS

Frontiers in Nutrition, Год журнала: 2023, Номер 10

Опубликована: Сен. 7, 2023

Sea buckthorn has a high nutritional value, but its sour taste and foul odor make it unpalatable for consumers. In this study, we analyzed the metabolite changes occurring during yeast-assisted fermentation of sea juice using HeadSpace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) Ultra-High Performance Liquid (UHPLC-MS) techniques. A total 86 volatile aroma compounds were identified process. The content volatiles in increased by 3469.16 μg/L after 18 h fermentation, with 22 showing elevated levels. Notably, esters fruity, floral, sweet aromas 1957.09 μg/L. We 379 non-volatile metabolites observed significant increases relative abundance key active ingredients fermentation: glycerophosphorylcholine (increased 1.54), glutathione 1.49), L-glutamic acid 2.46), vanillin 0.19). KEGG pathway analysis revealed that amino metabolism lipid primary metabolic pathways involved Saccharomyces cerevisiae. Fermentation been shown to improve flavor increase bioactive compounds. This study provides novel insights into dynamics following yeast through metabolomics analysis. These findings could serve as theoretical foundation further studies on factors influencing differences fermentation.

Язык: Английский

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