International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 11241 - 11241
Опубликована: Окт. 19, 2024
Traumatic
brain
injury
(TBI),
a
major
cause
of
death
and
disability
among
young
people,
leads
to
significant
public
health
economic
challenges.
Despite
its
frequency,
treatment
options
remain
largely
unsuitable.
However,
examination
the
blood–brain
barrier
(BBB)
can
assist
with
understanding
mechanisms
dynamics
dysfunction,
which
affects
TBI
sufferers
secondarily
injury.
Here,
we
present
rat
model
focused
on
two
standard
BBB
assessment
markers,
high-
low-molecular-weight
complexes,
in
order
understand
disruption.
In
addition,
tested
new
technique
evaluate
disruption
single
set,
comparing
neuroimaging.
A
total
100
Sprague–Dawley
rats
were
separated
into
following
five
groups:
naive
(n
=
20
rats),
control
administration
60
rats).
Rats
assessed
at
different
time
points
after
measure
using
low-
high-molecular-weight
complexes.
Neurological
severity
score
was
evaluated
baseline
24
h
TBI.
During
neurological
exam
TBI,
scanned
magnetic
resonance
imaging
euthanized
for
permeability.
We
found
that
markers
displayed
examples
same
set
tissues
over
period
week.
Our
innovative
protocol
assessing
permeability
complexes
showed
appropriate
results.
Additionally,
determined
lower
limit
sensitivity,
therefore
demonstrating
accuracy
this
method.
Reports of Morphology,
Год журнала:
2024,
Номер
30(1), С. 55 - 60
Опубликована: Март 17, 2024
Mild
blast-induced
traumatic
brain
injury
is
common
among
the
military,
resulting
in
cognitive
impairment,
reduced
socialization,
which
leads
to
disability
and,
as
a
result,
deterioration
quality
of
life.
It
considered
that
blood-brain
barrier
disruption
and
microvascular
dysfunction
are
key
this
type
injury.
The
purpose
study
was
changes
vessels
after
air
shock
wave
exposure.
carried
out
on
48
mature
male
Wistar
rats,
were
randomly
divided
into
2
groups:
an
experimental
group,
animals
subjected
inhalation
anesthesia
using
halothane
exposed
with
overpressure
26.4±3.6
kPa,
Sham
group.
After
simulation
days
1st,
3rd,
7th,
14th,
21st,
rats
euthanized
removed
all
standard
histological
procedures
stained
hematoxylin
eosin.
For
immunohistochemical
studies,
primary
antibodies
used
eNOS.
finished
preparations
examined
by
light
microscopy
photographed.
Disorders
cerebral
detected
from
day
1st
posttraumatic
period.
found
blast
led
vascular
rupture,
well
increased
permeability
diapedesis
red
blood
cells
edema
for
up
21st
days.
Focal
violations
wall
integrity
cortical
hippocampal
hemocapillaries,
venular
link
submembrane
vessels;
morphology
metabolic
endothelium;
uneven
filling
major
importance.
These
indicate
eNOS
expression
dilation
vessels,
compensatory
mechanism
response
improve
circulation.
However,
not
involved
vasodilation,
we
observed
post-trauma.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 11241 - 11241
Опубликована: Окт. 19, 2024
Traumatic
brain
injury
(TBI),
a
major
cause
of
death
and
disability
among
young
people,
leads
to
significant
public
health
economic
challenges.
Despite
its
frequency,
treatment
options
remain
largely
unsuitable.
However,
examination
the
blood–brain
barrier
(BBB)
can
assist
with
understanding
mechanisms
dynamics
dysfunction,
which
affects
TBI
sufferers
secondarily
injury.
Here,
we
present
rat
model
focused
on
two
standard
BBB
assessment
markers,
high-
low-molecular-weight
complexes,
in
order
understand
disruption.
In
addition,
tested
new
technique
evaluate
disruption
single
set,
comparing
neuroimaging.
A
total
100
Sprague–Dawley
rats
were
separated
into
following
five
groups:
naive
(n
=
20
rats),
control
administration
60
rats).
Rats
assessed
at
different
time
points
after
measure
using
low-
high-molecular-weight
complexes.
Neurological
severity
score
was
evaluated
baseline
24
h
TBI.
During
neurological
exam
TBI,
scanned
magnetic
resonance
imaging
euthanized
for
permeability.
We
found
that
markers
displayed
examples
same
set
tissues
over
period
week.
Our
innovative
protocol
assessing
permeability
complexes
showed
appropriate
results.
Additionally,
determined
lower
limit
sensitivity,
therefore
demonstrating
accuracy
this
method.
