Transcriptome analysis of two isolates of the tomato pathogen Cladosporium fulvum, uncovers genome-wide patterns of alternative splicing during a host infection cycle DOI Creative Commons
Alex Z. Zaccaron, Li‐Hung Chen, Ioannis Stergiopoulos

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(12), С. e1012791 - e1012791

Опубликована: Дек. 18, 2024

Alternative splicing (AS) is a key element of eukaryotic gene expression that increases transcript and proteome diversity in cells, thereby altering their responses to external stimuli stresses. While AS has been intensively researched plants animals, its frequency, conservation, putative impact on virulence, are relatively still understudied plant pathogenic fungi. Here, we profiled the events occurring genes Cladosporium fulvum isolates Race 5 4, during nearly complete compatible infection cycle tomato host. Our studies revealed extensive heterogeneity isoforms assembled from different isolates, infections, timepoints, as over 80% were singletons detected only single sample. Despite that, 40% protein-coding each isolate predicted be recurrently across disparate two isolates. Of these, 37.5% common both 59% resulted multiple protein isoforms, putatively increasing pathogen by 31% infections. An enrichment analysis showed mostly affected likely involved transport nutrients, regulation expression, monooxygenase activity, suggesting role for finetuning adaptation C . host Tracing location fungal chromosomes they located repeat-rich regions core chromosomes, indicating causal connection between genome propensity AS. Finally, cases differential isoform usage identified, modulation at stages may another way which pathogens refine infections hosts.

Язык: Английский

Sugars will eventually be exported transporter 18 mediates glucose metabolism in Torreya grandis kernels DOI
Shuya Wang,

Ruoman Wang,

H. Zhong

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143529 - 143529

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Bridging the gap: glucose transporters, Alzheimer’s, and future therapeutic prospects DOI Creative Commons
Mai Albaik,

Dalaa Sheikh Saleh,

Dana Kauther

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Янв. 16, 2024

Glucose is the major source of chemical energy for cell functions in living organisms. The aim this mini-review to provide a clearer and simpler picture fundamentals glucose transporters as well relationship these Alzheimer’s disease. This study was conducted accordance with Preferred Reporting Items Systematic Reviews Meta-Analyses (PRISMA). Electronic databases ( PubMed ScienceDirect ) were used search relevant studies mainly published during period 2018–2023. covers two main types transporters, facilitated (GLUTs) sodium-glucose linked (SGLTs). difference between that first type works through passive transport across concentration gradient. second active co-transportation against its Fluctuation translates into disturbance normal functioning, such disease, which may be caused by significant downregulation GLUTs most closely associated insulin resistance brain. sign lack GLUT4 translocation. tau hyperphosphorylation, GLUT1 3 being strongly upregulated. current focuses on use treating diseases because their proven therapeutic potential. Despite this, remain insufficient inconclusive due complex intertwined nature processes. recommends further understanding mechanisms related vectors promising future therapies.

Язык: Английский

Процитировано

8
Osmotic Pressure and Its Biological Implications DOI Open Access
Songjie Zheng, Yan Li,

Yingfeng Shao

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3310 - 3310

Опубликована: Март 14, 2024

Gaining insight into osmotic pressure and its biological implications is pivotal for revealing mechanisms underlying numerous fundamental processes across scales will contribute to the biomedical pharmaceutical fields. This review aims provide an overview of current understanding, focusing on two central issues: (i) how determine theoretically (ii) affects important activities. More specifically, we discuss representative theoretical equations models different solutions, emphasizing their applicability limitations, summarize effect lipid phase separation, cell division, differentiation, dependence these processes. We highlight that new theory applicable all experimentally feasible temperatures solute concentrations needs be developed, further studies regarding role in other should also carried out improve our comprehensive in-depth understanding. Moreover, point importance challenges developing techniques vivo measurement pressure.

Язык: Английский

Процитировано

8
Glucose transport, transporters and metabolism in diabetic retinopathy DOI Creative Commons
Chaoyang Zhang,

Limin Gu,

Hai Xie

и другие.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2023, Номер 1870(3), С. 166995 - 166995

Опубликована: Дек. 22, 2023

Язык: Английский

Процитировано

14
Impact of PET/CT Imaging with FDG in Locally Advanced Cervical Carcinoma—A Literature Review DOI Creative Commons
Ottó Molnar,

Oreste Straciuc,

Simona Mihuțiu

и другие.

Current Oncology, Год журнала: 2024, Номер 31(5), С. 2508 - 2526

Опубликована: Апрель 29, 2024

Positron emission tomography (PET) and computed (CT) have evolved as a pivotal diagnostic modality in the field of oncology. With its increasing application staging ready availability, it becomes imperative for committed radiation oncologists to possess complete analysis understanding integration molecular imaging, which can be helpful planning, while also acknowledging possible limitations challenges. A significant obstacle lies synthesis design tumor-specific bmolecules diagnosing treating cancer. The utilization medical biochemistry biotechnology, encompassing diagnosis, therapy, control biological systems, is encapsulated under umbrella term “nuclear medicine”. Notably, various radioisotopes pharmaceutics has garnered attention, particularly realm delivery systems drugs, DNA, imaging agents. present article provides comprehensive review use novel techniques PET CT with major positron-emitting radiopharmaceuticals currently progress or utilized clinical practice their into therapy.

Язык: Английский

Процитировано

5
The Effect of Olive Pomace in Different Levels and Vitamin E on (Cyprinus carpio L.) Diets on Physiological Blood Parameters DOI Open Access

Z HASSAN,

Hazem S. Abedalhammed

IOP Conference Series Earth and Environmental Science, Год журнала: 2025, Номер 1449(1), С. 012019 - 012019

Опубликована: Фев. 1, 2025

Abstract The experiment was carried out in fish’s laboratory belong to Department of Animal Production at College Agriculture / University Anbar for 56 days from (29 March 2024 24 May 2024). This research sought explore the role Olive pomace (Iraqi type green color) on haemato-biochemical indicators blood common carp ( Cyprinus carpio Linnaeus ). One-Hundred twenty fish with comparable weight were chosen conduct aquaria (dimensions 40 x 70 and height 30 cm) previously allocated them. After acclimatization, randomly distributed eight per aquarium three replicates treatment, initial (41.52 ± 4.4 gm.) fish, biomass coordinated a rate (334.49 1.25 g) one aquarium. Five diets protein content 32% prepared feed fish. first T1, control treatment (basal diet), T2 (adding olive 0.1%), T3 0.2%), T4(adding 0.3%) T5 (addition vitamin E 0.4%).

Язык: Английский

Процитировано

0
Molecular architectures of glycosylated dendronized bottle brushes in action: Biocompatibility and anti-amyloidogenic activity of pseudo-glycodendrimers DOI Creative Commons

Tom Kösterke,

Radika Thakore,

Sílvia Moreno

и другие.

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101771 - 101771

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Correlating sugar transporter expression and activities to identify transporters for an orphan sugar substrate DOI Creative Commons
Elisabeth Tamayo,

Basant Nada,

Isabell Hafermann

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1), С. 1 - 18

Опубликована: Янв. 8, 2024

Abstract Filamentous fungi like Neurospora crassa are able to take up and metabolize important sugars present, for example, in agricultural human food wastes. However, only a fraction of all putative sugar transporters filamentous has been characterized date, many substrates, the corresponding unknown. In N. , 14 out 42 major facilitator superfamily (MFS)–type have so far. To uncover this hidden potential biotechnology, it is therefore necessary find new strategies. By correlation uptake profile interest after different induction conditions with expression profiles 44 genes encoding predicted together an exhaustive phylogenetic analysis using sequences fungal transporters, we aimed identify transporter candidates tested sugars. Following approach, found high rates strengths dedicated galacturonic acid arabinose, while loose that transported by several due functional redundancy. Nevertheless, combinatorial approach allowed us elucidate system disaccharide lactose, by-product dairy industry, which consists two main cellodextrin CDT-1 CDT-2 minor contribution related NCU00809. Moreover, non-MFS involved glycerol transport was also identified. Deorphanization or identification orphan substrates kinetics information can thus provide way optimize reuse industry by-products wastes order create economic value reduce their environmental impact. Key points • The genome contains 30 uncharacterized genes. Correlation help substrates. CDT-1, CDT-2, NCU00809 key players lactose .

Язык: Английский

Процитировано

2
A Comprehensive Review of Membrane Transporters and MicroRNA Regulation in Alzheimer’s Disease DOI
Shatakshi Mishra,

B. Stany,

Anushka Das

и другие.

Molecular Neurobiology, Год журнала: 2024, Номер unknown

Опубликована: Апрель 1, 2024

Язык: Английский

Процитировано

2
Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells DOI Open Access

Yue Zhou,

Shuhao Zhang,

Yurong Jia

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5155 - 5155

Опубликована: Май 9, 2024

Adiponectin is an important adipokine involved in glucose and lipid metabolism, but its secretion potential role regulating utilization during ovarian development remains unclear. This study aims to investigate the mechanism effects of follicle-stimulating hormones (FSHs) on adiponectin following impact transport granulosa cells rat ovaries. A range experimental techniques were utilized test our research, including immunoblotting, immunohistochemistry, immunofluorescence, ELISA, histological staining, real-time quantitative PCR, transcriptome analysis. The immunohistochemistry results indicated that was primarily located In primary cultured vitro, both Western blot immunofluorescence assays demonstrated FSH significantly induced within 2 h incubation, via PKA signaling pathway rather than PI3K/AKT pathway. Concurrently, addition AdipoR1/AdipoR2 dual agonist AdipoRon culture medium stimulated protein expression GLUT1 cells, resulting enhanced absorption. Consistent with these vitro findings, rats injected eCG (which shares structural functional similarities FSH) exhibited increased levels ovaries blood. Moreover, there a notable elevation mRNA AdipoRs GLUTs administration. Transcriptomic analysis further revealed positive correlation between intraovarian system transporter. present represents novel investigation, demonstrating stimulates through potentially influences (GLUT1)

Язык: Английский

Процитировано

2