Identification and Characterization of Neuroprotective Properties of Thaumatin-like Protein 1a from Annurca Apple Flesh Polyphenol Extract

Nutrients, Год журнала: 2024, Номер 16(2), С. 307 - 307

Опубликована: Янв. 19, 2024

Background: Alzheimer’s disease (AD) and Parkinson’s (PD) are multifactorial neurodegenerative disorders that mostly treated with drugs inhibiting key enzymes of cholinergic aminergic neurotransmission, such as acetyl butyryl cholinesterase (AChE, BuChE) or monoamine oxidases (MAO)-A/B, Aβ 1–40 aggregation. Diet plant components multitarget functions promising compounds in the prevention AD PD. Our aim was to identify neuroprotective from Annurca apple polyphenol extract (AFPE). Methods: AFPE fractionated by gel filtration, eluted peaks were subjected chemical analyses (i.e., RP-HPLC mass spectrometry), determination inhibitory enzyme activity cell effects MTT, morphology assays. Results: In AFPE, we identified thaumatin-like protein 1a, belonging pathogenesis-related (PR) family. This showed best on AChE, MAO-A (IC50 = 5.53 µM 1.71 µM, respectively), Aβ1–40 fibril aggregation 9.16 µM), compared other polyphenol-containing fractions. Among latter, Peak 4 reverted formation 104.87 µM). Moreover, 1a protected AGS MKN-28 cells serum-deprivation-induced stress conditions. Conclusions: We exerted not only through its polyphenols but also which acted like a molecule.

Язык: Английский

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Associations between Mediterranean Diet Adherence, Quality of Life, and Mental Health in Patients with Multiple Sclerosis: A Cross-Sectional Study

Journal of Personalized Medicine, Год журнала: 2024, Номер 14(2), С. 199 - 199

Опубликована: Фев. 11, 2024

Background: The Mediterranean diet (MD) is well-known as a which may exert protective effect against neurodegenerative diseases, including multiple sclerosis (MS). To date, only few clinical surveys have assessed the potential effects of MD in patients with MS. purpose present study to evaluate compliance on disease disability, quality life, physical activity, depressive symptomatology, and blood biochemical parameters related nutritional status MS patients, considering several socio-demographic, anthropometric, lifestyle characteristics. Methods: This cross-sectional conducted 558 adults aged 18–64 years. Relevant questionnaires were utilized socio-demographic anthropometric parameters, disability (Expanded Disability Status Scale, EDSS), multidimensional health-related (MS Quality Life-54, MSQOL-54), activity levels (International Physical Activity Questionnaire, IPAQ), depression (Beck Depression Inventory II, BDI-II), adherence (MedDietScore), while retrieved from patients’ medical records. Results: Enhanced was independently associated decreased frequency overweight/obesity, well abdominal obesity, suffering Elevated also incidence advanced higher prevalence elevated an improved lower symptoms, certain are effective indicators iron deficiency malnutrition. Conclusions: found that slow down promoting better life mental health Future prospective required obtain conclusive results.

Язык: Английский

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A novel Mediterranean diet-inspired supplement ameliorates cognitive, microbial, and metabolic deficits in a mouse model of low-grade inflammation

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Июнь 4, 2024

The Mediterranean diet (MD) and its bioactive constituents have been advocated for their neuroprotective properties along with capacity to affect gut microbiota speciation metabolism. Mediated through the brain axis, this modulation of may partly contribute MD. To explore potential interaction, we evaluated a novel blend (Neurosyn240) resembling in rodent model chronic low-grade inflammation. Behavioral tests cognition, proteomic analysis, 16S rRNA sequencing, 1H NMR metabolomic analyses were employed develop an understanding gut-brain axis interactions involved. Recognition memory, as assessed by object recognition task (NOR), decreased response LPS insult was restored Neurosyn240 supplementation. Although open field performance did not reach significance, it correlated NOR indicating element anxiety related cognitive change. changes associated accompanied shift composition which included restoration Firmicutes: Bacteroidota ratio increase Muribaculum, Rikenellaceae Alloprevotella, most notably Akkermansia significantly performance. also metabolites 5-aminovalerate, threonine, valine, uridine monophosphate, adenosine turn profile within dramatically influenced both interventions, KEGG analysis highlighting oxidative phosphorylation neurodegenerative disease-related pathways be modulated. Intriguingly, subset these simultaneously abundance predominantly phosphorylation, perhaps alluding protective interaction. Collectively, our results suggest that conferred resilience deleterious effects

Язык: Английский

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Transgenerational hormesis in healthy aging and antiaging medicine from bench to clinics: Role of food components

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 220, С. 111960 - 111960

Опубликована: Июль 5, 2024

Язык: Английский

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Plant-based probiotic foods: current state and future trends

Food Science and Biotechnology, Год журнала: 2024, Номер 33(15), С. 3401 - 3422

Опубликована: Авг. 28, 2024

Язык: Английский

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Improved nutritional and antioxidant properties of black soldier fly larvae reared on spent coffee grounds and blood meal by-products

Food Research International, Год журнала: 2024, Номер 196, С. 115151 - 115151

Опубликована: Сен. 27, 2024

Язык: Английский

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Exploring the effect of different diet types on ageing and age-related diseases

Nutrition, Год журнала: 2024, Номер 129, С. 112596 - 112596

Опубликована: Окт. 10, 2024

Язык: Английский

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Antioxidant, Anti-Alzheimer’s, anticancer, and cytotoxic properties of peanut oil: in vitro, in silico, and GC-MS analysis

Frontiers in Chemistry, Год журнала: 2024, Номер 12

Опубликована: Окт. 24, 2024

Introduction Peanut oil is valued for its mild flavor, rich phytochemical content, therapeutic potential, and associated health benefits. This study aims to analyze the chemical composition, antioxidant properties, anti-Alzheimer’s potential of Algerian peanut using both experimental computational approaches. The goal evaluate suitability pharmaceutical applications, particularly antioxidant, anti-Alzheimer, anticancer properties. Methods composition was determined Gas Chromatography-Mass Spectrometry (GC-MS). Antioxidant activity assessed through DPPH CUPRAC assays, while enzyme inhibition evaluated butyrylcholinesterase (BChE) assays. In silico molecular docking studies were conducted predict interactions between key compounds BChE. Additionally, physicochemical properties Lipinski’s rule five, cytotoxicity tested against various cancer cell lines, including melanoma (A2058 SK-MEL-1), non-small lung (NCI-H838), leukemia (H9). Results GC-MS identified 20 in oil, with oleic acid as predominant compound (41.98%). showed an IC50 value 265.96 ± 14.85 μg/mL assay. BChE moderate, 36.47% 3.71% at 200 μg/mL. Molecular highlighted 6-methyl octahydro-coumarin a score −15.86 kJ/mol BChE, although it less potent than Galantamine (−23.4 kJ/mol). Physicochemical analysis revealed that palmitic exhibit logP values 5.71 5.20, respectively, indicating drug-like potential. Cytotoxicity assessments demonstrated acid, stearic effective cells, oxiraneoctanoic 3-octyl, significant cells. Discussion conclusion results demonstrate possesses notable high concentration alongside moderate strong cytotoxic effects on highlights agent. While exhibited favorable scores, lower effectiveness compared suggests further optimization required enhanced efficacy. These findings underscore development, like emerging promising candidates continued research drug development. from Algeria holds promise future applications neuroprotective, therapies.

Язык: Английский

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MicroRNAs and the Mediterranean diet: a nutri-omics perspective for lung cancer

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Июль 7, 2024

Abstract Lung cancer is the deadliest type worldwide with ~ 1.8 million deaths per-year. Smoking accounts for 85% of all cases, a described joint effect unhealthy diet in lung risk increase. Public health policies to prevent carcinogens exposure, promote smoking cessation and advocacy healthy nutrition, are therefore highly recommended. Here we have examined benefits Mediterranean Diet (MedDiet) protecting against some non-communicable diseases including cancer, highlighting epidemiological biomolecular aspects MedDiet anti-inflammatory its interaction habits closely linked cancer. Considering high incidence mortality rates discussed also about global impact that Planeterranean extension could on controlling risk. We debated personalized nutrition prevention, considering individual heterogeneity response plans as well recent advancements nutri-omics research, specific focus role microRNAs (miRNAs) promising nutritional molecular hub prevention. strongly believe deep understanding link between food components genetic/epigenetics factors can expand effective intervention strategies.

Язык: Английский

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Secondary Bioactive Metabolites from Foods of Plant Origin as Theravention Agents against Neurodegenerative Disorders

Foods, Год журнала: 2024, Номер 13(14), С. 2289 - 2289

Опубликована: Июль 20, 2024

Neurodegenerative disorders (NDDs) such as Alzheimer’s (AD) and Parkinson’s (PD) are on the rise, robbing people of their memories independence. While risk factors age genetics play an important role, exciting studies suggest that a diet rich in foods from plant origin may offer line defense. These kinds foods, namely fruits vegetables, packed with plethora powerful bioactive secondary metabolites (SBMs), including terpenoids, polyphenols, glucosinolates, phytosterols capsaicinoids, which exhibit wide range biological activities antioxidant, antidiabetic, antihypertensive, anti-Alzheimer’s, antiproliferative, antimicrobial properties, associated preventive effects development chronic diseases mediated by oxidative stress type 2 diabetes mellitus, respiratory diseases, cancer, cardiovascular NDDs. This review explores potential SBMs theravention agents (metabolites therapeutic action) against By understanding science behind plant-based prevention, we be able to develop new strategies promote brain health prevent rise The proposed stands out emphasizing integration multiple preventing Previous research has often focused individual compounds or specific but this aims present comprehensive fingerprint how various can synergistically contribute health. related NDD diagnostic process, addition some examples food-related products medicinal plants significantly reduce inhibition acetylcholinesterase (AChE), butyrylcholinesterase (BChE), β-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1), highlighted.

Язык: Английский

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