Yeast models of mutations in NFU1 gene for biochemical characterization and drug screening
Biochemical and Biophysical Research Communications,
Год журнала:
2025,
Номер
763, С. 151760 - 151760
Опубликована: Апрель 12, 2025
Язык: Английский
Kcs1 and Vip1: The Key Enzymes behind Inositol Pyrophosphate Signaling in Saccharomyces cerevisiae
Biomolecules,
Год журнала:
2024,
Номер
14(2), С. 152 - 152
Опубликована: Янв. 26, 2024
The
inositol
pyrophosphate
pathway,
a
complex
cell
signaling
network,
plays
pivotal
role
in
orchestrating
vital
cellular
processes
the
budding
yeast,
where
it
regulates
cycle
progression,
growth,
endocytosis,
exocytosis,
apoptosis,
telomere
elongation,
ribosome
biogenesis,
and
stress
responses.
This
pathway
has
gained
significant
attention
pharmacology
medicine
due
to
its
generating
pyrophosphates,
which
serve
as
crucial
molecules
not
only
but
also
higher
eukaryotes.
As
targets
for
therapeutic
development,
genetic
modifications
within
this
hold
promise
disease
treatment
strategies,
offering
practical
applications
biotechnology.
model
organism
Saccharomyces
cerevisiae,
renowned
tractability,
been
instrumental
various
studies
related
pathway.
review
is
focused
on
Kcs1
Vip1,
two
enzymes
involved
biosynthesis
of
S.
highlighting
their
roles
processes,
providing
an
up-to-date
overview
relationship
with
phosphate
homeostasis.
Moreover,
underscores
potential
these
findings
realms
biotechnology,
profound
implications
comprehending
intricate
network.
Язык: Английский
In Silico Exploration of APOE4 Inhibitors: Molecular Docking and ADMET Profiling for Alzheimer's Therapy
Journal of Health and Rehabilitation Research,
Год журнала:
2024,
Номер
4(1), С. 652 - 658
Опубликована: Фев. 13, 2024
Background:
Alzheimer's
disease
(AD),
a
prevalent
neurodegenerative
disorder,
poses
significant
challenges
to
healthcare
systems
worldwide.
The
aggregation
of
Amyloid-β
peptide
and
the
presence
apolipoprotein
E4
(apoE4)
are
recognized
as
central
factors
in
AD
pathogenesis.
Despite
availability
FDA-approved
drugs,
there
remains
dire
need
for
more
effective
targeted
treatments
combat
this
disease.
advent
silico
methodologies
offers
promising
avenue
accelerating
drug
discovery
process,
providing
cost-effective
efficient
means
screen
potential
therapeutic
agents.
Objective:
This
study
aimed
identify
evaluate
inhibitors
apoE4
using
methods.
By
targeting
interaction
domain
apoE4,
sought
discover
compounds
capable
reducing
beta-amyloid
aggregation,
thus
offering
novel
approach
therapy.
Methods:
Computational
techniques,
including
Lipinski
Rule
Five
ADMET
filtering,
were
employed
dataset
80
chemical
retrieved
from
NCBI
database.
Molecular
docking
was
performed
assess
binding
affinities
orientations
utilizing
Phyre2
3D
structure
prediction
AutoDock
Vina
simulations.
selection
criteria
lead
based
on
their
energy,
hydrogen
bonding
interactions,
compliance
with
drug-like
properties.
Results:
Out
initial
screened,
15
excluded
Five,
an
additional
43
deemed
unsuitable
following
analysis.
identified
two
compounds,
taxifolin
luteolin,
exhibiting
superior
showing
lowest
energy
-7.4
kcal/mol
highest
number
bonds
one
its
conformations.
Conclusion:
successfully
leveraged
methods
luteolin
potent
highlighting
computational
developing
therapies.
These
findings
pave
way
future
experimental
validation
clinical
trials
efficacy
safety
these
treating
Язык: Английский
Saccharomyces cerevisiae as a Model for Studying Human Neurodegenerative Disorders: Viral Capsid Protein Expression
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(24), С. 17213 - 17213
Опубликована: Дек. 7, 2023
In
this
article,
we
briefly
describe
human
neurodegenerative
diseases
(NDs)
and
the
experimental
models
used
to
study
them.
The
main
focus
is
yeast
Saccharomyces
cerevisiae
as
an
model
processes.
We
review
recent
data
on
aggregation
of
disease-related
proteins
in
cells.
addition,
results
studies
that
were
designed
investigate
molecular
mechanisms
underlie
reporter
proteins.
advantages
disadvantages
approaches
are
currently
formation
protein
aggregates
described.
Special
attention
given
similarity
between
form
a
result
misfolding
viral
factories-special
structural
formations
which
particles
formed
inside
virus-infected
A
separate
part
devoted
our
previously
published
upon
expression
insect
densovirus
capsid
Based
reviewed
NDs
related
aggregation,
well
new
system
for
proposed.
core
proposed
comparative
transcriptomic
analysis
changes
signaling
pathways
during
Язык: Английский