Biochemical Genetics, Год журнала: 2024, Номер unknown
Опубликована: Фев. 9, 2024
Язык: Английский
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12
Опубликована: Май 28, 2024
The repair of irregular bone tissue suffers severe clinical problems due to the scarcity an appropriate therapeutic carrier that can match dynamic and complex damage. Fortunately, stimuli-responsive in situ hydrogel systems are triggered by a special microenvironment could be ideal method regenerating because injectability, gelatin, spatiotemporally tunable drug release. Herein, we introduce two main stimulus-response approaches, exogenous endogenous, forming hydrogels engineering. First, summarize specific distinct responses extensive range external stimuli (e.g., ultraviolet, near-infrared, ultrasound, etc.) form created from biocompatible materials modified various functional groups or hybrid nanoparticles. Furthermore, “smart” hydrogels, which respond endogenous physiological environmental temperature, pH, enzyme, etc.), achieve gelation one injection vivo without additional intervention. Moreover, mild chemistry response-mediated also offer fascinating prospects engineering, such as Diels–Alder, Michael addition, thiol-Michael Schiff reactions, etc. recent developments challenges smart their application administration engineering discussed this review. It is anticipated advanced strategies innovative ideas will exploited field increase quality life for patients with
Язык: Английский
Процитировано
10
Gels, Год журнала: 2025, Номер 11(4), С. 271 - 271
Опубликована: Апрель 5, 2025
Collagen-based wound dressings have developed as an essential component of contemporary care, utilizing collagen’s inherent properties to promote healing. This review thoroughly analyzes collagen dressing advances, examining different formulations such hydrogels, films, and foams that enhance care. The important processes by which promotes healing (e.g., promoting angiogenesis, encouraging cell proliferation, offering structural support) are discussed clarify its function in tissue regeneration. effectiveness adaptability demonstrated via clinical applications investigated acute chronic wounds. Additionally, commercially accessible collagen-based skin treatments discussed, demonstrating their practical use healthcare settings. Despite the progress, study discusses obstacles restrictions encountered producing adopting dressings, difficulties manufacturing financial concerns. Finally, current landscape’s insights indicate future research possibilities for optimization, bioactive agent integration, overcoming existing constraints. analysis highlights potential innovations improve treatment methods patient
Язык: Английский
Процитировано
1
Journal of Clinical Medicine, Год журнала: 2024, Номер 13(10), С. 3005 - 3005
Опубликована: Май 20, 2024
Background: Although osteoarthritis (OA) development is epidemiologically multifactorial, a primary underlying mechanism still under debate. Understanding the pathophysiology of OA remains challenging. Recently, experts have focused on autophagy as contributor to development. Method: To better understand pathogenesis OA, we survey literature role and molecular mechanisms identify relevant studies, used controlled vocabulary free text keywords search MEDLINE, EMBASE, Cochrane Central Register Controlled Trials, Web Science, SCOPUS database. Thirty-one studies were included for data extraction systematic review. Among these twenty-five investigated effects in aging chondrocytes, six examined normal human only one study mechanical stress-induced chondrocytes. Results: The suggest that activation prevents by exerting cell-protective However, intricate, certain indicate stimulating cells can cytotoxic effect, while others propose it may protective (cytoprotective) effect against damage or degeneration. Conclusions: Mechanical also thought be involved but further research required precise mechanism. Thus, contributions should interpreted with caution types cartilage.
Язык: Английский
Процитировано
7
Frontiers in Pharmacology, Год журнала: 2025, Номер 15
Опубликована: Янв. 2, 2025
Objective This study aimed to identify the potential active compounds in Rhizoma Musae decoction and understand their mechanisms of action osteoarthritis treatment. Methods UHPLC-Q-Exactive-MS/MS technology was used for an in-depth analysis chemical present decoction. A network approach construct a comprehensive compounds, targets, pathways, which provided insights into molecular Results The integrated revealed presence 534 decoction, with 7beta-hydroxyrutaecarpine, 7,8-dihydroxycoumarin, pinocembrin diacetate, scopoletin being identified as compounds. Potential targets such GAPDH, AKT1, TNF, IL6, SRC were implicated key pathways including MAPK signaling pathway, lipid atherosclerosis, PI3K-Akt IL-17 pathway. Molecular docking studies showed significant binding affinity between core components. In vitro cell experiments have demonstrated that RM can enhance proliferation upregulates expression TNFα, IL-6, SRC, while down-regulating GAPDH AKT1. Conclusion influence specific involved pathogenesis, providing new functional development utilization RM.
Язык: Английский
Процитировано
0
Frontiers in Medicine, Год журнала: 2025, Номер 12
Опубликована: Янв. 21, 2025
Knee osteoarthritis (OA) is a common condition that causes pain and reduces the quality of life for many people. It also leads to high health financial costs. Managing knee OA requires using different methods together best results. This review overviews current therapeutic options pain, focusing on their efficacy, safety, potential roles in clinical practice. Topical treatments, such as NSAIDs capsaicin, offer significant relief with minimal systemic side effects are suitable initial therapy, nonpharmacologic interventions like exercise and, when relevant, weight loss. Oral analgesics, including acetaminophen opioids, have limited efficacy serious effects, making them appropriate only short-term or rescue therapy. Intra-articular injections, corticosteroids, hyaluronic acid, platelet rich plasma, demonstrate varying levels safety. Nutritional supplements, curcumin, Boswellia serrata , glucosaminechondroitin combinations, modest benefits used adjuncts standart treatment. Nonpharmacological transcutaneous electrical nerve stimulation (TENS), acupuncture, local heat provide variable should be customized based individual patient responses. Targeted biologic agents, antibodies TNF-α, IL-1, NGF, hold promise more precise relief; however, further research required establish routine use. Treating personalized, combining several methods. Research must continue improve treatments make safer.
Язык: Английский
Процитировано
0
Arthritis Research & Therapy, Год журнала: 2025, Номер 27(1)
Опубликована: Янв. 24, 2025
To explore IL11 co-expression profiles in our previously reported RNA-sequencing dataset of OA articular cartilage, interaction with IL6, and to investigate the effects hrIL11 administration as potential therapeutic strategy for cartilage using biomimetic aged human osteochondral explant model OA. We used datasets macroscopically preserved lesioned (N = 35 patients). Spearman correlations were calculated between IL6 expression levels genes expressed 20048 genes). Osteochondral explants isolated from areas joint kept culture two weeks, or without exposure 200ng/ml hrIL11. found no overlap correlating indicating their distinct roles cartilage. Moreover, we identified more being correlated compared 203 106 genes, respectively). Upon treatment ex vivo hrIL11, overall observed unbeneficial on chondrocyte phenotype, illustrated by upregulation MMP13, EPAS1, RUNX2, POSTN. did not observe significant differences Mankin scores upon addition The current study showed that is unlikely be beneficial despite previous indications druggable target. These findings underscore importance functionally investigating risk genes. Better understanding signaling underlying pathways necessary towards development strategy.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140473 - 140473
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2025, Номер 13(3), С. 570 - 570
Опубликована: Фев. 24, 2025
This narrative review provides an overview of the various diagnostic tools used to assess cartilage health, with a focus on early detection, nutrition intervention, and management osteoarthritis. Early detection damage is crucial for effective patient management. Traditional like radiography conventional magnetic resonance imaging (MRI) sequences are more suited detecting late-stage structural changes. paper highlights advanced techniques, including sodium MRI, T2 mapping, T1ρ imaging, delayed gadolinium-enhanced MRI cartilage, which provide valuable biochemical information about composition, particularly glycosaminoglycan content its potential links nutrition-related factors influencing health. Cartilage degradation often linked inflammation measurable via markers CRP IL-6 which, although not specific breakdown, offer insights into affecting cartilage. In addition markers, such as collagen breakdown products aggrecan fragments, reflect metabolic changes in discussed. Emerging optical coherence tomography hybrid positron emission tomography–magnetic (PET-MRI) also explored, offering high-resolution combined insights, respectively. Finally, wearable technology biosensors real-time monitoring osteoarthritis progression, well role artificial intelligence enhancing accuracy through pattern recognition data addressed. While these hold great osteoarthritis, challenges remain clinical translation, validation larger populations integration existing workflows personalized treatment strategies cartilage-related diseases.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1901 - 1901
Опубликована: Фев. 22, 2025
Inflammation plays a critical role in the pathogenesis of osteoarthritis (OA). The objective this study was to investigate anti-inflammatory and chondroprotective properties Artemisia annua L. water extract (AWE) following induction inflammation cartilage cells (SW1353 cell) through administration interleukin-1 beta (IL-1β). We demonstrated significant antioxidant activity, as evidenced by elevated total phenolic flavonoid content, addition robust free radical scavenging capacity, assessed DPPH (2,2-diphenyl-1-picrylhydrazyl) ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Its cytotoxic effects were at concentration 200 μg/mL, where no signs observed SW1353 treated with IL-1β; levels reactive oxygen species (ROS) notably reduced dose-dependent manner. principal inflammatory markers, cyclooxygenase-2 (COX-2) inducible nitric oxide synthase (iNOS), significantly diminished AWE treatment. led reduction expression key proteins involved mitogen-activated protein kinase (MAPK) nuclear factor kappa-light-chain-enhancer activated B cell (NF-κB) signaling pathways, ultimately resulting decrease release matrix metalloproteinases (MMPs), specifically MMP-1 MMP-13, which are known contribute degradation. Additionally, degraded collagen type II restored. These findings suggest that reducing oxidative stress inflammation, along inhibiting MAPK NF-κB may ameliorate progression IL-1β-induced OA. Furthermore, molecular docking analysis revealed strong binding affinity mediator Six compounds identified AWE, corroborating its potential effects. Therefore, serve potentially useful therapeutic agent against OA modulating inflammation-related mechanisms.
Язык: Английский
Процитировано
0
Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 4319 - 4334
Опубликована: Март 1, 2025
The primary pathology of rheumatoid arthritis (RA) involves the invasion extracellular matrix (ECM) articular cartilage by inflammation-activated fibroblast-like synoviocytes (FLS), a process targeted most RA therapeutic drugs. However, absence an efficient in vitro model for evaluating FLS hinders relevant drug screening and mechanism research. To address this, novel three-dimensional (3D) chondrocytic spheroid that mimics ECM has been developed, along with corresponding indices to quantify invasion. matrigel-free 3D was developed using ultra-low attachment plate. characterized transcriptome sequencing, immunofluorescent staining. explore feasibility this invasive capacity synoviocytes, multi-interference strategies, including ADAMTS5 gene overexpression, inflammatory cytokine stimulation, anti-inflammatory (Etanercept) treatment were involved. Additionally, specific indices-Invasion Depth Ratio (IDR), Invasion Counts (IC), (IR), Area (IAR)-were designed culture environment is more suitable synthesis increasing anabolism-related (COL2A1) reducing catabolism-related genes (ADAMTS5, MMP3, CCL2 CDKN2A) expression. Moreover, optimal conditions developing identified. This sensitive gene, inflammation interference. Increased IDR, IC, IR IAR observed overexpressed- IL-1β-treated spheroid. Further, Etanercept could inhibit TNF-α induced offers ideal platform innovative pathogenesis studies focused on cartilage.
Язык: Английский
Процитировано
0