Food Chemistry, Год журнала: 2024, Номер 469, С. 142558 - 142558
Опубликована: Дек. 19, 2024
Язык: Английский
Journal of Ethnopharmacology, Год журнала: 2024, Номер 328, С. 118015 - 118015
Опубликована: Март 16, 2024
Язык: Английский
Процитировано
3
FEBS Journal, Год журнала: 2024, Номер 291(20), С. 4619 - 4632
Опубликована: Авг. 15, 2024
We genetically manipulated HaCaT cells, a spontaneously immortalised normal keratinocyte cell line, to stably express two different coloured luciferase reporter genes, driven by interleukin 8 ( IL‐8 ) and ubiquitin‐C UBC promoters, respectively. Subsequently, we generated three‐dimensional (3D) skin‐like in vitro composite (SLIC) utilising these with the objective of monitoring bioluminescence emitted from SLIC. This SLIC was on non‐woven silica fibre membranes differentiation medium. Immunohistochemical analyses skin markers revealed expression keratins 2 10, filaggrin, involucrin, indicating mature characteristics. engineered employed for real‐time monitoring, allowing assessment time‐ dose‐dependent responses UV stress, as well hydrophilic hydrophobic chemical loads. Notably, evaluation substances has been challenging conventional 2D culture methods, suggesting need new approach, which this technology could address. Our observations suggest that constitutively expressing reporters selected promoters are tailored specific objectives, significantly facilitates assays exploring physiological functions cells based genetic response mechanisms. It also highlights avenues evaluating impacts various compounds designed topical application human skin.
Язык: Английский
Процитировано
1
Journal of Functional Foods, Год журнала: 2024, Номер 116, С. 106209 - 106209
Опубликована: Апрель 27, 2024
Язык: Английский
Процитировано
0
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown
Опубликована: Сен. 24, 2024
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Окт. 14, 2024
Excess exogenous supplementation of D-galactose (D-gal), a monosaccharide and reducing sugar, generates reactive oxygen species (ROS), leading to cell damage death. ROS accumulation is critical in aging. Therefore, D-gal-induced aging mouse models are used studies. Herein, we evaluated D-gal's effect on neonatal testis development using an vitro organ culture method. Mouse testicular fragments (MTFs) derived from testes (postnatal day 5) were cultured with 500 mM D-gal for 5 days. D-gal-treated MTFs showed significantly increased decreased expression undifferentiated differentiated germ markers, respectively, substantial reduction meiotic cells. In D-gal-exposed MTFs, levels Sertoli markers (Sox9 Wt1) increased, while those StAR 17β-HSD3, whose expressions abundant D-Gal treated adult Leydig cells, decreased. Additionally, the enzyme 3 β-HSD1, essential steroidogenesis was reduced compared that controls.D-gal Bad, Bax, cleaved caspase-3 -8. Via oxidative stress MTF. Overall, negatively regulates through pro-apoptotic mechanisms ROS.
Язык: Английский
Процитировано
0
Synthetic Communications, Год журнала: 2024, Номер unknown, С. 1 - 8
Опубликована: Дек. 15, 2024
An efficient method to synthesize functionalized allyl isothiocyanates in moderate yields has been demonstrated, based on the isomerization of Morita-Baylis-Hillman adduct-derived allylic thiocyanates. This methodology offers obvious advantages such as easy purification, atom economy, and a broad scope substrates.
Язык: Английский
Процитировано
0
Food Chemistry, Год журнала: 2024, Номер 469, С. 142558 - 142558
Опубликована: Дек. 19, 2024
Язык: Английский
Процитировано
0