Interdisciplinary cancer research, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Life Sciences, Год журнала: 2024, Номер 359, С. 123227 - 123227
Опубликована: Ноя. 12, 2024
Язык: Английский
Процитировано
0
medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Май 22, 2024
Summary We developed a technology for detecting and quantifying trace nucleic acids using bracketing protocol designed to yield copy number with approximately ± 20% accuracy across all concentrations. The microRNAs (miRNAs) let-7b, miR-15b, miR-21, miR-375 miR-141 were measured in serum urine samples from healthy subjects patients breast, prostate, or pancreatic cancer. Detection quantification amplification-free enabled osmium-tagged probes MinION, nanopore array detection device. Combined men (Sigma‒Aldrich #H6914) was used as reference. Total RNA isolated biospecimens commercial kits the miRNA source. unprecedented led conclusion that numbers must be normalized same content, which turn illustrates (i) independence age, sex, ethnicity, well (ii) equivalence between urine. copies cancers 1.8-fold overexpressed, exhibited zero overlap had p value of 1.6×10 -22 , tentatively validating each cancer biomarker. miR-15b confirmed cancer-independent, whereas let-7b appeared biomarker prostate breast cancer, but not
Язык: Английский
Процитировано
0
IntechOpen eBooks, Год журнала: 2024, Номер unknown
Опубликована: Июль 11, 2024
Since the development and evolution of COVID-19 immunization, use mRNA-based technologies has led to revolutionary changes due potential RNA-based therapies, which are believed be useful in treating many infectious diseases. Information on treatment hepatitis C virus (HCV) following this rule highlights therapeutic microRNAs (miRNAs). The advent direct-acting antivirals (DAAs) changed paradigm HCV treatment. However, challenges remain, particularly areas viral resistance, genetic diversity, chronic Among these, miRNAs a sensible approach complementing improving existing models. implementation new non-coding RNAs should investigated. This chapter discusses public awareness RNA (ncRNA) strategies against HCV. From modification discovery pathways focusing their applications, efficacy, As scientific community looks toward antiviral drugs, demonstrates that introduction drugs into health systems holds promise for addressing providing solutions such as drug persistence, more. New therapy not only expand scope but also define landscape increase flexibility adaptability face challenges.
Язык: Английский
Процитировано
0
Gene, Год журнала: 2024, Номер 933, С. 149011 - 149011
Опубликована: Окт. 18, 2024
Systemic lupus erythematosus (SLE) is a complex autoimmune disease marked by abnormal B-cell proliferation and increased autoantibodies. miRNAs play crucial role in regulating dysfunction SLE pathology. influence DNA methylation, activation, gene expression, contributing to pathogenesis. impact B cells through key processes like proliferation, differentiation, tolerance, apoptosis. also exacerbate inflammation immune responses modulating Interleukin 4 (IL-4), IL-6, interferon cytokines. Autophagy, degradation mechanism, regulated specific that This article explores the of multiple development, survival, responses, influencing miR-23a, miR-17 ∼ 92 family, miR-125b/miR-221 affect development transcription factors, signaling pathways, cell cycle genes. such as miR-181a-5p miR-23a-5p are differentially across developmental stages, emphasizing their regulatory roles biology. synthesizes miRNA-B interactions offer new strategies directions for diagnosis treatment.
Язык: Английский
Процитировано
0
Interdisciplinary cancer research, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0